INTRODUCTION

Top Abstract Introduction Materials and Methods Results and Discussion References

The genus Yersinia contains 11 species, of which Yersinia enterocolitica, Y. pseudotuberculosis, and Y. pestis are known to be pathogenic for humans. The species Y. enterocolitica consists of enteropathogenic and nonpathogenic strains, the latter belonging mostly to biogroup 1A (11, 12). Biogroup 1A strains of Y. enterocolitica are ubiquitous and are most frequently isolated from the environment, e.g., water, soil, and plant surfaces (26, 38); from food (8, 18); or from pigs, which are an important reservoir for food-borne infections of humans with pathogenic Y. enterocolitica O:3 and O:9 strains (19, 20). All pathogenic strains of Y. enterocolitica as well as the pathogenic species Y. pestis and Y. pseudotuberculosis possess a conserved 70-kb plasmid carrying essential virulence genes, e.g., for Yersinia outer proteins (7). Plasmids similar in size to the virulence plasmid are also present in biogroup 1A strains of Y. enterocolitica (31, 39, 41). Only scant information regarding the properties of these plasmids is available. Hybridization studies revealed, in some cases, partial homologies to the virulence plasmid (25). However, the well-known plasmid-encoded virulence genes were not detected on the plasmids of biogroup 1A strains (17). Nevertheless, the occurrence of pathogenic strains and biogroup 1A strains in the same host, the isolation of biogroup 1A strains from clinical samples, and the detected plasmid homologies suggest the possibility of horizontal gene transfer between these strains.

Gene exchange by transduction with temperate phages might be widespread in Y. enterocolitica, as 70 to 85% of isolates of this species, on average, have been reported to be lysogenic (28, 30). In addition, phages lytic for Yersinia strains can be readily isolated from sewage (2, 6, 9). In spite of the narrow host range of many Y. enterocolitica phages, which is useful for the differentiation of Yersinia strains by phage typing (3), some phages have a wide host range and even lyse members of other genera, e.g., Enterobacter, Escherichia, and Klebsiella (37). Moreover, temperate phages of Y. frederiksenii, Y. intermedia, and Y. kristensenii have been reported to have a wider host range than those of Y. enterocolitica and are often able to infect pathogenic strains of Y. enterocolitica (10). However, only 9% of the strains of these nonpathogenic Yersinia species are considered lysogenic (40). Finally, until now, naturally occurring lysogeny has never been reported for Y. pseudotuberculosis and Y. pestis, although infection by phages isolated from the environment has been observed (3).

Apart from host range analysis and some electron microscopic studies (21, 22), only scant information and no molecular data about Yersinia phages are available. In addition, it is unknown what role Yersinia phages might play in gene transfer in natural environments. Therefore, we have isolated a temperate phage from a pathogenic Y. enterocolitica strain and a mixture of Yersinia phages from sewage. Transduction experiments revealed that the temperate phage as well as the Yersinia phages from sewage are able to transduce small plasmids isolated from Y. enterocolitica biogroup 1A strains.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results and Discussion References

Bacterial strains. All Yersinia strains used in this study were obtained from the strain collection of the Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, and the strain collection of the Robert Koch-Institut. The nonpathogenic Yersinia strains were originally isolated from environmental sources (freshwater and manure), food, animals (pigs, cattle, chickens, sheep, dogs, and wild animals), and humans. The pathogenic Yersinia strains were mainly isolated from diseased pigs and humans (25). The bacteria were routinely grown in Luria-Bertani (LB) medium at 28°C.

Isolation of bacteriophages. The temperate bacteriophage PY20 was isolated from biogroup 4 serogroup O:3 strain Y. enterocolitica 29820 by mitomycin C (Sigma Chemical Co., St. Louis, Mo.) induction. Briefly, bacteria were grown in 8 ml of LB medium in the dark. During the early logarithmic growth phase, mitomycin C (1.5 to 2.5 µg ml¹) was added to the culture, and the optical density at 588 nm was measured every hour and additionally after incubation for 24 h. Bacteria were sedimented (5,000 × g, 20 min, 4°C), and the supernatant was passed through a sterile filter (0.2-µm-pore size). The cell lysate was examined for plaque-forming activity by dropping 20 µl of the lysate onto LB soft agar containing growing Yersinia indicator strains. To isolate phages from raw sewage, water samples were obtained from two sewage treatment plants in Brandenburg, Germany. A 90-ml portion of each water sample was centrifuged (5,000 × g, 20 min, 4°C), and the supernatants were passed through 0.45-µm-pore-size filters. Phages were sedimented by ultracentrifugation (112,000 × g, 2 h, 10°C), resuspended in 3 ml of SM buffer (33), and passed through 0.2-µm-pore-size filters. The plaque-forming activity on Yersinia strains was investigated by the spot test described above. Phages were isolated from the areas of lysis on agar plates by removing some agar and resuspending it in 500 µl of SM buffer. Dilutions of these phage cocktails were plated on the respective indicator strains to isolate single plaques. Single phages and phage mixtures were propagated in order to obtain high-titer lysates for DNA extraction and transduction experiments (see below).

Phage propagation, purification, and isolation of phage DNA. Starting from a single plaque, phages were propagated by the soft-agar overlay method described by Sambrook et al. (33). In order to obtain high-titer lysates, soft agar from 20 agar plates with confluent lysis was harvested. The agar was resuspended in 200 ml of SM buffer by stirring for at least 2 h at room temperature. Bacteria and debris were removed by centrifugation (9,000 × g, 10 min, 4°C) and subsequent filtration (0.2 µm). The phages were concentrated by ultracentrifugation (112,000 × g, 90 min) and purified with cesium chloride step gradients (33). The CsCl was removed by filtration with Centricon 500 filtration units (Amicon, Witten, Germany). The phages were resuspended in 1 ml of SM buffer. In order to isolate phage DNA, phage lysates were mixed with an equal volume of phenol, vortexed for 30 s, incubated for 1 min at room temperature, and vortexed again for 30 s. The subsequent centrifugation and extraction steps with phenol-chloroform and chloroform and precipitation of the phage DNA were performed by standard procedures (33).

Analysis of phage DNA. Restriction analysis was performed with restriction endonucleases according to the manufacturer's (MBI Fermentas, St. Leon Roth, Germany) recommendations. DNA fragments were separated on 0.8% agarose gels in TBE buffer (33). Hybridization studies with fluorescein-labelled DNA probes were performed with a Renaissance Kit from NEN Dupont as outlined by the manufacturer.

Electron microscopy. CsCl-purified phage particles were adsorbed to carbon films and negatively stained with 1% uranyl acetate (pH 4.5) or 2% potassium phosphowolframate (pH 7.2) as described by Steven et al. (36). The samples were examined with a Philips CM 100 electron microscope. The droplet technique described by Lang and Mitani (24) was used for the determination of phage genome size. The 7,250-bp cloning vector m13mp18 (New England BioLabs) was used as an internal standard.

Preparation of plasmid and chromosomal DNAs. Plasmid DNA of Yersinia strains was isolated by a procedure based on the alkaline lysis method described by Birnboim and Doly (5). Chromosomal DNA was purified from bacterial cells with a MasterPure Genomic DNA Purification Kit (Epicentre Technologies, Madison, Wis.).

Generalized transduction. The transduction experiments were performed with the following plasmids: p13169neo (virulence plasmid of O:3 strain Y. enterocolitica 13169 [25]) and p29807neo and p49neo (small plasmids originally isolated from Y. enterocolitica biogroup 1A strains) (see Fig. 2). All plasmids contained a neomycin resistance gene inserted by use of the mini-Tn5 transposon derivative pUTKm described by De Lorenzo et al. (13) and Herrero et al. (16). In order to create suitable donor strains for the transduction experiment, both small plasmids were transformed by electroporation into O:3 strain Y. enterocolitica 13169/2, which was cured of its virulence plasmid (15). After infection of the donor strains, high-titer lysates were prepared (see above). Wild-type strain 13169 was used as a recipient for lysates of the donor strains containing the marked small plasmids. The cured derivative 13169/2 was used as a recipient for the lysate of the donor strain containing the marked virulence plasmid. The recipient strains were infected by the isolated phages at a multiplicity of infection of about 1 and plated on LB agar supplemented with 100 µg of neomycin per ml. As controls, the phage lysates as well as the recipient strains were plated on the same medium. Colonies which appeared on the agar plates were investigated by comparison of their plasmid patterns with those of the donor and recipient strains, by DNA hybridization with the respective plasmids and with phage PY20, and by phage induction (see above).

	RESULTS AND DISCUSSION

Top Abstract Introduction Materials and Methods Results and Discussion References

Characteristics of the temperate phage PY20. Phage PY20 was induced in a pathogenic Y. enterocolitica strain which had been isolated from a diseased pig. The phage showed typical morphological features of Myoviridae, a phage family exhibiting a long contractile tail with a core (Fig. 1). The morphology of Y. enterocolitica phages belonging to the Myoviridae family has been previously described (21). The average length of phage PY20 particles was 186 nm (n = 4). The average sizes of the elongated heads and tails were 71 by 59 nm and 106 by 19 nm, respectively, in good agreement with the published data (21). The genomes of tailed phages generally consist of double-stranded DNA (1). The genome size of phage PY20 was determined by restriction enzyme analysis and by electron microscopy. After digestion with HpaI, 13 DNA fragments ranging from 0.8 to 7.7 kb were obtained, and a molecular size of approximately 46 kb was estimated. By electron microscopy, a genome size of 51 ± 0.9 kb (n = 8) was determined. Only linear genome structures were detected, indicating that phage PY20 does not possess cohesive ends. This finding was confirmed by treatment of the phage DNA with T4 ligase prior to digestion or heating of the DNA after digestion. No change in the restriction pattern was observed in either case.

View larger version (131K): [in this window] [in a new window]   FIG. 1.   Electron micrograph of temperate phage PY20 isolated from Y. enterocolitica. Bar, 100 nm.

Characteristics of phages isolated from sewage. The lytic activity of the phage mixtures on growing Yersinia indicator strains was analyzed by a spot test. Altogether, 62 pathogenic and nonpathogenic Yersinia strains were investigated. The spot test revealed that the phage cocktails were lytic for a wide range of Yersinia strains (Table 1). In most cases, large clear areas of lysis were visible. After resuspension of agar from the areas of lysis and plating of dilutions on the respective indicator strain, many more clear plaques than turbid plaques were obtained. This finding indicated that the sewage samples contained mainly virulent phages. To elucidate if single phages in the sewage samples were able to infect pathogenic and nonpathogenic Yersinia strains, we isolated phages from 14 individual plaques and investigated their host range. Thirteen phages were isolated from plaques on pathogenic Y. enterocolitica strains (O:3 and O:5,27), and 1 phage was isolated from a plaque on a Y. pseudotuberculosis strain. Of the 14 isolated phages, 11 were not active on nonpathogenic Y. enterocolitica biogroup 1A strains. The three remaining phages, which were isolated from plaques on Y. enterocolitica O:5,27 strains, lysed the nonpathogenic biogroup 1A serogroup O:5 strains. One of these phages was also lytic for Y. enterocolitica O:3 strains.

Generalized transduction of Yersinia plasmids. In order to study the capabilities of phage PY20 and of phage mixtures from sewage to transduce Yersinia plasmids, a test system comprising donor and recipient strains as well as marked plasmids was established. In these experiments, we investigated the potential of the phages to transduce the virulence plasmid p13169neo (ca. 72 kb) and two small plasmids isolated from biogroup 1A strains of Y. enterocolitica (p29807neo [4.3 kb] and p49neo [5.8 kb]) (Fig. 2). Until now, we could not detect any phenotypic traits conferred by the small plasmids, although the total DNA sequence of p29807 was determined (accession no. AJ132618; EMBL, Cambridge, United Kingdom).

View larger version (14K): [in this window] [in a new window]   FIG. 2.   Restriction endonuclease cleavage maps of p29807neo and p49neo. nptII, neomycin resistance gene.

5

6

5

7

View larger version (48K): [in this window] [in a new window]   FIG. 3.   Plasmid profiles of the Y. enterocolitica donor strains and recipient strain used for generalized transduction and of two transductants. Lanes: 1, marker DNA (phage lambda DNA digested with HindIII); 2, Y. enterocolitica 13169 (recipient); 3, Y. enterocolitica 13169(p49neo) (donor); 4, transductant containing p49neo; 5, Y. enterocolitica 13169 (p29807neo) (donor); 6, transductant containing p29807neo.

View larger version (83K): [in this window] [in a new window]   FIG. 4.   Southern hybridization of phage PY20 DNA with total DNA from Yersinia strains. All DNAs were digested with HindIII and separated on an 0.8% agarose gel followed by Southern blotting. Lanes: 1, PY20; 2, empty; 3, Y. enterocolitica 29820 (original host strain of PY20); 4, Y. enterocolitica 13169 (recipient for the transduction experiments); 5 to 9, total DNA of five transductants.

5

7