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Applied and Environmental Microbiology, October 2000, p. 4555-4558, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid and Simple Determination of the Escherichia coli Phylogenetic Group

Olivier Clermont, Stéphane Bonacorsi, and Edouard Bingen*

Laboratoire d'études de génétique bactérienne dans les infections de l'enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France

Received 15 February 2000/Accepted 7 July 2000


    ABSTRACT
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Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.


    TEXT
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Escherichia coli is a normal inhabitant of the intestines of most animals, including humans. Some E. coli strains can cause a wide variety of intestinal and extra-intestinal diseases, such as diarrhea, urinary tract infections, septicemia, and neonatal meningitis (18). Phylogenetic analyses have shown that E. coli strains fall into four main phylogenetic groups (A, B1, B2, and D) (10, 21) and that virulent extra-intestinal strains belong mainly to group B2 and, to a lesser extent, to group D (4, 7, 12, 19), whereas most commensal strains belong to group A. These studies have also given us a better understanding of how pathogenic strains acquire virulence genes (4). Actually, phylogenetic grouping can be done by multilocus enzyme electrophoresis (10, 20) or ribotyping (2-4, 8), but both of these reference techniques are complex and time-consuming and also require a collection of typed strains.

Creation of a subtractive library for two E. coli strains belonging to different phylogenetic groups (6) and characterization of an anonymous 14.9-kb fragment strongly associated with neonatal meningitis strains (3) suggested that certain genes or DNA fragments might be specific phylogenetic group markers. Three candidate markers were studied further: (i) chuA, a gene required for heme transport in enterohemorrhagic O157:H7 E. coli (6, 16, 23, 24); (ii) yjaA, a gene initially identified in the recent complete genome sequence of E. coli K-12, the function of which is unknown (5); and (iii) an anonymous DNA fragment designated TSPE4.C2 from our subtractive library (6). Here we describe a rapid technique for determining the phylogenetic groups of E. coli strains based on PCR detection of the chuA and yjaA genes and DNA fragment TSPE4.C2. The method was evaluated by testing 230 strains that had already been grouped by using reference methods.

Bacterial strains and growth conditions. The 72 strains of the ECOR collection (17) were kindly provided by R. Selander (Department of Biology, University of Rochester, Rochester, N.Y.). These reference strains, isolated from a variety of hosts and geographic locations, are representative of the range of genotypic variation in the species. Sixty-eight of these strains belong to the four main phylogenetic groups (A, B1, B2, and D), and four are unclassified (10, 21). We also tested a set of 86 E. coli strains causing neonatal meningitis (ECNM strains) (4), 34 E. coli strains responsible for neonatal septicemia without meningitis, 30 E. coli strains isolated from feces of healthy neonates, the J96 uropathogenic E. coli strain (O4:K6) (kindly provided by J. Hacker, Institut für Molekulare Infektionsbiologie, Würzburg, Germany) (22), and 10 verotoxin-producing E. coli O157:H7 strains, including 1 strain obtained from A. D. O'Brien (Uniformed Services University of the Health Sciences, Bethesda, Md.) and 9 strains isolated from different locations in France (P. Mariani, Hôpital Robert-Debré, Paris, France). The phylogenetic group distribution of 69 of the 86 ECNM strains has been published previously (4). The other 17 ECNM strains and the 75 remaining clinical isolates were classified by ribotyping as previously described (4). The E. coli laboratory K-12 strain MG1655, which belongs to phylogenetic group A, was also studied (10). Bacteria were grown at 37°C in Luria-Bertani broth or on Luria-Bertani agar. When necessary, ampicillin (100 µg per ml) was used.

PCR amplification and Southern blotting. As a first step, PCR was performed with a standard protocol. Each reaction was carried out by using a 20-µl mixture containing 2 µl of 10× buffer (supplied with Taq polymerase), 20 pmol of each primer, each deoxynucleoside triphosphate at a concentration of 2 µM, 2.5 U of Taq polymerase (ATGC Biotechnologie, Noisy-le-Grand, France), and 200 ng of genomic DNA. The PCR was performed with a Perkin-Elmer GeneAmp 9600 thermal cycler with MicroAm tubes under the following conditions: denaturation for 5 min at 94°C; 30 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C; and a final extension step of 7 min at 72°C. The primer pairs used were ChuA.1 (5'-GACGAACCAACGGTCAGGAT-3') and ChuA.2 (5'-TGCCGCCAGTACCAAAGACA-3'), YjaA.1 (5'-TGAAGTGTCAGGAGACGCTG-3') and YjaA.2 (5'-ATGGAGAATGCGTTCCTCAAC-3'), and TspE4C2.1 (5'-GAGTAATGTCGGGGCATTCA-3') and TspE4C2.2 (5'-CGCGCCAACAAAGTATTACG-3'), which generate 279-, 211-, and 152-bp fragments, respectively. In a simplified protocol, a two-step triplex polymerase reaction based on previously described methods (1, 9) was assessed. The components of the reaction mixture were the same as those in the standard protocol, except that (i) DNA was directly provided by 3 µl of bacterial lysate or a piece of a colony, (ii) the six above-mentioned primers were mixed, and (iii) the PCR steps were as follows: denaturation for 4 min at 94°C, 30 cycles of 5 s at 94°C and 10 s at 59°C, and a final extension step of 5 min at 72°C.

Southern blotting was performed by capillary transfer to positively charged nylon membranes. Hybridization was performed at 65°C in 1% sodium dodecyl sulfate-1 M NaCl-50 mM Tris HCl (pH 7.5)-1% blocking reagent (Boehringer, Mannheim, Germany). The membranes were washed in 2× SSC for 15 min at room temperature, then in 2× SSC-0.1% sodium dodecyl sulfate for 30 min at 65°C, and finally in 0.1× SSC for 5 min at room temperature (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate). Chemiluminescence detection was performed according to the manufacturer's instructions (DIG Luminescent Detection Kit for nucleic acids; Boehringer). The probes were produced by PCR according to the manufacturer's instructions (PCR DIG Probe Synthesis Kit; Boehringer) by using the primers and amplification procedure described above for the standard protocol.

PCR grouping results. A total of 230 strains were analyzed. Their phylogenetic groups, as determined by reference methods, were as follows: 43 belonged to group A, 23 belonged to group B1, 51 belonged to group D, and 113 belonged to group B2. Table 1 shows the PCR results for the entire set of strains according to their phylogenetic groups. The chuA gene was present in all strains belonging to groups B2 and D and was absent from all strains belonging to groups A and B1. This allowed us to separate groups B2 and D from groups A and B1. In the same way, the yjaA gene allowed perfect discrimination between group B2 (100% of the strains were positive) and group D (100% of the strains were negative). Finally, clone TSPE4.C2 was present in all but two of the group B1 strains and absent from all group A strains. All the PCR results were confirmed by Southern hybridization (data not shown). The results of these three amplifications made it possible to establish a dichotomous decision tree (Fig. 1) for phylogenetic grouping. With this tree, 228 of the 230 strains tested (99%) were correctly grouped, while only 2 strains were wrongly classified (group B1 strains were identified as group A strains). Identical results were obtained with standard and simplified PCR protocols. Figure 2 shows the different profiles obtained by triplex PCR for the four phylogenetic groups.

                              
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TABLE 1.   PCR amplification of the chuA and yjaA genes and DNA fragment TSPE4.C2 in E. coli strains from various collections according to phylogenetic group



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FIG. 1.   Dichotomous decision tree to determine the phylogenetic group of an E. coli strain by using the results of PCR amplification of the chuA and yjaA genes and DNA fragment TSPE4.C2.


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FIG. 2.   Triplex PCR profiles specific for E. coli phylogenetic groups. Each combination of chuA and yjaA gene and DNA fragment TSPE4.C2 amplification allowed phylogenetic group determination of a strain. Lanes 1 and 2, group A; lane 3, group B1; lanes 4 and 5, group D; lanes 6 and 7, group B2. Lane M contained markers.

In this work, we developed a PCR method to rapidly determine the phylogenetic groups of E. coli strains and obtained an accuracy of more than 99% compared to the reference method. Phylogenetic characterization of E. coli strains on the basis of a very few phenotypic or genotypic features initially appeared to be very difficult. Such genotypic traits (presence or absence of a gene, for example) must meet different criteria for use in phylogenetic characterization. First, the gene must have been acquired or deleted when the group that it characterizes emerged. Second, the same gene must have been "stabilized," thereby ruling out its subsequent deletion or horizontal transfer among bacteria belonging to other phylogenetic groups. Finally, recombination events in the candidate gene must be very rare. In other words, the gene product must not be targeted by natural selection, which favors new genetic recombinations (24). Previous attempts to identify specific phylogenetic group characteristics based on phenotypic (21) or genotypic features (11) were not sufficiently discriminative. For the first time, we describe the use of two genes and an anonymous DNA fragment in a simple phylogenetic grouping method. Too little information is available on yjaA and the DNA fragment to speculate on their evolutionary history. In contrast, the study by Wyckoff et al. (25) of heme transport genes, together with our results, suggests that chuA was acquired by sister groups B2 and D (15) soon after their emergence rather than being present in a common ancestor and subsequently being lost by groups B1 and A.

However, two strains (ECOR 70 and an ECNM strain) belonging to phylogenetic group B1 were classified in group A by our method. This discrepancy may be explained by an intermediate genetic base between these two groups in these strains and by the fact that the region studied with our method (chuA, yjaA, and TSPE4.C2 are located at 78.7 min [25], 90.8 min [5], and approximately 87 min [6], respectively, in relation to the genome of E. coli K-12) may be more closely related to group A regions than to the regions studied with the reference methods. Indeed, it has been demonstrated that groups A and B1 are sister groups (15). Moreover, recent multiple chromosomal nucleotide sequence analysis has shown that ECOR 70 may be considered a "hybrid" strain, in which some housekeeping genes exhibit nucleotide sequences shared by group A ECOR strains and some other genes exhibit nucleotide sequences shared by group B1 ECOR strains (15; E. Denamur, personal communication). Thus, the phylogenetic group of ECOR 70 remains to be settled. In addition to the rapidity of our PCR-based method, no reference collection is required, meaning that the assay can easily be used in any laboratory. Furthermore, in contrast to other methods, group allocation is unequivocal. Indeed, the four unclassified strains in the ECOR collection, ECOR 31, ECOR 37, ECOR 42, and ECOR 43, were classified by our method; the first three strains belong to group D, and the fourth belongs to group A. It is noteworthy that all the sequences of the housekeeping genes studied in the latter strain were characteristic of those found in group A strains (Denamur, personal communication).

In conclusion, our simple and rapid phylogenetic grouping technique could have several practical applications. The first is in bioclinical practice, given the established link between phylogenetic group and virulence (4, 7, 12, 19). The second is as a biotechnological screening tool for eliminating potentially pathogenic strains when candidate strains for cloning are screened. Such screening tools have been developed, for example, to identify E. coli K-12 strains by PCR (13) or to detect E. coli strains with no known virulence genes by a reverse dot blot procedure described by Kuhnert et al. (14). Our method has the advantage of being capable of identifying nonpathogenic strains other than E. coli K-12 and suitable for large-scale strain screening. Thus, after all strains belonging to groups B2 and D, which are potentially pathogenic, are eliminated, the reverse dot blot technique (14) could be applied to group A or B1 strains to eliminate rare strains harboring virulence factors.


    ACKNOWLEDGMENTS

We thank Walter Nanni and Colin Tinsley for technical help and Xavier Nassif, Jacques Elion, and Erick Denamur for helpful discussions.

This work was supported in part by the Programme de Recherche Fondamentale en Microbiologie, Maladies Infectieuses et Parasitaires (Appel d'offre 1998), "Recherche de déterminants génétiques de pathogénicité chez E. coli K1 responsable de méningite néonatale," and by the Programme Hospitalier de Recherche Clinique (grant AOM 96069).


    FOOTNOTES

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Robert Debré, 48 blvd. Sérurier, 75395 Paris cedex 19, France. Phone: 33 1 40 03 23 40. Fax: 33 1 40 03 24 50. E-mail: edouard.bingen{at}rdb.ap-hop-paris.fr.


    REFERENCES
Top
Abstract
Text
References

1. Belgrader, P., W. Benett, D. Hadley, G. Long, R. Mariella, Jr., F. Milanovich, S. Nasarabadi, W. Nelson, J. Richards, and P. Stratton. 1998. Rapid pathogen detection using a microchip PCR array instrument. Clin. Chem. 44:2191-2194[Abstract/Free Full Text].
2. Bingen, E., E. Denamur, and J. Elion. 1994. Use of ribotyping in epidemiological surveillance of nosocomial outbreaks. Clin. Microbiol. Rev. 7:311-317[Abstract/Free Full Text].
3. Bingen, E., E. Denamur, N. Brahimi, and J. Elion. 1996. Genotyping may provide rapid identification of Escherichia coli K1 organisms that cause neonatal meningitis. Clin. Infect. Dis. 22:152-156[Medline].
4. Bingen, E., B. Picard, N. Brahimi, S. Mathy, P. Desjardins, J. Elion, and E. Denamur. 1998. Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. J. Infect. Dis. 177:642-650[Medline].
5. Blattner, F. R., G. I. Plunkett, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1461[Abstract/Free Full Text].
6. Bonacorsi, S. P. P., O. Clermont, C. Tinsley, I. Le Gall, J. C. Beaudoin, J. Elion, X. Nassif, and E. Bingen. 2000. Identification of regions of the Escherichia coli chromosome specific for neonatal meningitis-associated strains. Infect. Immun. 68:2096-2101[Abstract/Free Full Text].
7. Boyd, E. F., and D. L. Hartl. 1998. Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution. J. Bacteriol. 180:1159-1165[Abstract/Free Full Text].
8. Desjardins, P., B. Picard, B. Kaltenböck, J. Elion, and E. Denamur. 1995. Sex in Escherichia coli does not disrupt the clonal structure of the population: evidence from random amplified polymorphic DNA and the restriction fragment length polymorphism. J. Mol. Evol. 41:440-448[CrossRef][Medline].
9. Haedicke, W., H. Wolf, W. Ehret, and U. Reischl. 1996. Specific and sensitive two-step polymerase reaction assay for the detection of the Salmonella species. Eur. J. Clin. Microbiol. Infect. Dis. 15:603-607[CrossRef][Medline].
10. Herzer, P. J., S. Inouye, M. Inouye, and T. S. Whittam. 1990. Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli. J. Bacteriol. 172:6175-6181[Abstract/Free Full Text].
11. Hurtado, A., and F. Rodriguez-Valera. 1999. Accessory DNA in the genomes of representatives of the Escherichia coli reference collection. J. Bacteriol. 181:2548-2554[Abstract/Free Full Text].
12. Johnson, J. R., and A. L. Stell. 2000. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J. Infect. Dis. 181:261-272[CrossRef][Medline].
13. Kuhnert, P., J. Nicolet, and J. Frey. 1995. Rapid and accurate identification of Escherichia coli K-12 strains. Appl. Environ. Microbiol. 61:4135-4139[Abstract].
14. Kuhnert, P., J. Hacker, I. Mühldorfer, A. P. Burnens, J. Nicolet, and J. Frey. 1997. Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains. Appl. Environ. Microbiol. 63:703-709[Abstract].
15. Lecointre, G., L. Rachdi, P. Darlu, and E. Denamur. 1998. Escherichia coli molecular phylogeny using the incongruence length difference test. Mol. Biol. Evol. 15:1685-1695[Abstract].
16. Mills, M., and S. Payne. 1995. Genetics and regulation of haem iron transport in Shigella dysenteriae and detection of an analogous system in Escherichia coli O157:H7. J. Bacteriol. 177:3004-3009[Abstract/Free Full Text].
17. Ochman, H., and R. K. Selander. 1984. Standard reference strains of Escherichia coli from natural populations. J. Bacteriol. 157:690-692[Abstract/Free Full Text].
18. Orskov, F., and I. Orskov. 1992. Escherichia coli serotyping and disease in man and animals. Can. J. Microbiol. 38:699-704[Medline].
19. Picard, B., J. S. Garcia, S. Gouriou, P. Duriez, N. Brahimi, E. Bingen, J. Elion, and E. Denamur. 1999. The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect. Immun. 67:546-553[Abstract/Free Full Text].
20. Selander, R. K., D. A. Caugant, H. Ochman, J. M. Mussser, M. N. Gilmour, and T. S. Whittam. 1986. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51:873-884[Free Full Text].
21. Selander, R. K., D. A. Caugant, and T. S. Whittam. 1987. Genetic structure and variation in natural populations of Escherichia coli, p. 1625-1648. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
22. Swenson, D. L., N. O. Bukanov, D. E. Berg, and R. A. Welch. 1996. Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. Infect. Immun. 64:3736-3743[Abstract].
23. Torres, A., and S. Payne. 1997. Haem iron-transport system in enterohaemorrhagic Escherichia coli O157:H7. Mol. Microbiol. 23:825-833[CrossRef][Medline].
24. Whittam, T. 1996. Genetic variation and evolutionary processes in natural populations of Escherichia coli, p. 2708-2720. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
25. Wyckoff, E. E., D. Duncan, A. G. Torres, M. Mills, K. Maase, and S. M. Payne. 1998. Structure of the Shigella dysenteriae haem transport locus and its phylogenetic distribution in enteric bacteria. Mol. Microbiol. 28:1139-1152[CrossRef][Medline].


Applied and Environmental Microbiology, October 2000, p. 4555-4558, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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  • Bogaerts, P., Rodriguez-Villalobos, H., Laurent, C., Deplano, A., Struelens, M. J., Glupczynski, Y. (2009). Emergence of extended-spectrum-AmpC-expressing Escherichia coli isolates in Belgian hospitals. J Antimicrob Chemother 63: 1073-1075 [Full Text]  
  • Deschamps, C., Clermont, O., Hipeaux, M. C., Arlet, G., Denamur, E., Branger, C. (2009). Multiple acquisitions of CTX-M plasmids in the rare D2 genotype of Escherichia coli provide evidence for convergent evolution. Microbiology 155: 1656-1668 [Abstract] [Full Text]  
  • Christenson, J. K., Gordon, D. M. (2009). Evolution of colicin BM plasmids: the loss of the colicin B activity gene. Microbiology 155: 1645-1655 [Abstract] [Full Text]  
  • Zhao, L., Gao, S., Huan, H., Xu, X., Zhu, X., Yang, W., Gao, Q., Liu, X. (2009). Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model. Microbiology 155: 1634-1644 [Abstract] [Full Text]  
  • Moodley, A., Guardabassi, L. (2009). Transmission of IncN Plasmids Carrying blaCTX-M-1 between Commensal Escherichia coli in Pigs and Farm Workers. Antimicrob. Agents Chemother. 53: 1709-1711 [Abstract] [Full Text]  
  • Literacka, E., Bedenic, B., Baraniak, A., Fiett, J., Tonkic, M., Jajic-Bencic, I., Gniadkowski, M. (2009). blaCTX-M Genes in Escherichia coli Strains from Croatian Hospitals Are Located in New (blaCTX-M-3a) and Widely Spread (blaCTX-M-3a and blaCTX-M-15) Genetic Structures. Antimicrob. Agents Chemother. 53: 1630-1635 [Abstract] [Full Text]  
  • Lobos, O., Padilla, C. (2009). Phenotypic characterization and genomic DNA polymorphisms of Escherichia coli strains isolated as the sole micro-organism from vaginal infections. Microbiology 155: 825-830 [Abstract] [Full Text]  
  • Takahashi, A., Muratani, T., Yasuda, M., Takahashi, S., Monden, K., Ishikawa, K., Kiyota, H., Arakawa, S., Matsumoto, T., Shima, H., Kurazono, H., Yamamoto, S. (2009). Genetic Profiles of Fluoroquinolone-Resistant Escherichia coli Isolates Obtained from Patients with Cystitis: Phylogeny, Virulence Factors, PAIusp Subtypes, and Mutation Patterns. J. Clin. Microbiol. 47: 791-795 [Abstract] [Full Text]  
  • Schierack, P., Romer, A., Jores, J., Kaspar, H., Guenther, S., Filter, M., Eichberg, J., Wieler, L. H. (2009). Isolation and Characterization of Intestinal Escherichia coli Clones from Wild Boars in Germany. Appl. Environ. Microbiol. 75: 695-702 [Abstract] [Full Text]  
  • Bielaszewska, M., Prager, R., Vandivinit, L., Musken, A., Mellmann, A., Holt, N. J., Tarr, P. I., Karch, H., Zhang, W. (2009). Detection and Characterization of the Fimbrial sfp Cluster in Enterohemorrhagic Escherichia coli O165:H25/NM Isolates from Humans and Cattle. Appl. Environ. Microbiol. 75: 64-71 [Abstract] [Full Text]  
  • Ewers, C., Antao, E.-M., Diehl, I., Philipp, H.-C., Wieler, L. H. (2009). Intestine and Environment of the Chicken as Reservoirs for Extraintestinal Pathogenic Escherichia coli Strains with Zoonotic Potential. Appl. Environ. Microbiol. 75: 184-192 [Abstract] [Full Text]  
  • Mammeri, H., Galleni, M., Nordmann, P. (2009). Role of the Ser-287-Asn Replacement in the Hydrolysis Spectrum Extension of AmpC {beta}-Lactamases in Escherichia coli. Antimicrob. Agents Chemother. 53: 323-326 [Abstract] [Full Text]  
  • Pomba, C., da Fonseca, J. D., Baptista, B. C., Correia, J. D., Martinez-Martinez, L. (2009). Detection of the Pandemic O25-ST131 Human Virulent Escherichia coli CTX-M-15-Producing Clone Harboring the qnrB2 and aac(6')-Ib-cr Genes in a Dog. Antimicrob. Agents Chemother. 53: 327-328 [Full Text]  
  • Poeta, P., Radhouani, H., Igrejas, G., Goncalves, A., Carvalho, C., Rodrigues, J., Vinue, L., Somalo, S., Torres, C. (2008). Seagulls of the Berlengas Natural Reserve of Portugal as Carriers of Fecal Escherichia coli Harboring CTX-M and TEM Extended-Spectrum Beta-Lactamases. Appl. Environ. Microbiol. 74: 7439-7441 [Abstract] [Full Text]  
  • Leflon-Guibout, V., Blanco, J., Amaqdouf, K., Mora, A., Guize, L., Nicolas-Chanoine, M.-H. (2008). Absence of CTX-M Enzymes but High Prevalence of Clones, Including Clone ST131, among Fecal Escherichia coli Isolates from Healthy Subjects Living in the Area of Paris, France. J. Clin. Microbiol. 46: 3900-3905 [Abstract] [Full Text]  
  • Johnson, T. J., Wannemuehler, Y., Doetkott, C., Johnson, S. J., Rosenberger, S. C., Nolan, L. K. (2008). Identification of Minimal Predictors of Avian Pathogenic Escherichia coli Virulence for Use as a Rapid Diagnostic Tool. J. Clin. Microbiol. 46: 3987-3996 [Abstract] [Full Text]  
  • Johnson, J. R., Clabots, C., Kuskowski, M. A. (2008). Multiple-Host Sharing, Long-Term Persistence, and Virulence of Escherichia coli Clones from Human and Animal Household Members. J. Clin. Microbiol. 46: 4078-4082 [Abstract] [Full Text]  
  • Johnson, T. J., Wannemuehler, Y., Johnson, S. J., Stell, A. L., Doetkott, C., Johnson, J. R., Kim, K. S., Spanjaard, L., Nolan, L. K. (2008). Comparison of Extraintestinal Pathogenic Escherichia coli Strains from Human and Avian Sources Reveals a Mixed Subset Representing Potential Zoonotic Pathogens. Appl. Environ. Microbiol. 74: 7043-7050 [Abstract] [Full Text]  
  • Vinue, L., Saenz, Y., Somalo, S., Escudero, E., Moreno, M. A., Ruiz-Larrea, F., Torres, C. (2008). Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain. J Antimicrob Chemother 62: 934-937 [Abstract] [Full Text]  
  • Zong, Z., Partridge, S. R., Thomas, L., Iredell, J. R. (2008). Dominance of blaCTX-M within an Australian Extended-Spectrum {beta}-Lactamase Gene Pool. Antimicrob. Agents Chemother. 52: 4198-4202 [Abstract] [Full Text]  
  • Rijavec, M., Muller-Premru, M., Zakotnik, B., Zgur-Bertok, D. (2008). Virulence factors and biofilm production among Escherichia coli strains causing bacteraemia of urinary tract origin. J Med Microbiol 57: 1329-1334 [Abstract] [Full Text]  
  • Fricke, W. F., Wright, M. S., Lindell, A. H., Harkins, D. M., Baker-Austin, C., Ravel, J., Stepanauskas, R. (2008). Insights into the Environmental Resistance Gene Pool from the Genome Sequence of the Multidrug-Resistant Environmental Isolate Escherichia coli SMS-3-5. J. Bacteriol. 190: 6779-6794 [Abstract] [Full Text]  
  • Karami, N., Hannoun, C., Adlerberth, I., Wold, A. E. (2008). Colonization dynamics of ampicillin-resistant Escherichia coli in the infantile colonic microbiota. J Antimicrob Chemother 62: 703-708 [Abstract] [Full Text]  
  • Cavaco, L. M., Abatih, E., Aarestrup, F. M., Guardabassi, L. (2008). Selection and Persistence of CTX-M-Producing Escherichia coli in the Intestinal Flora of Pigs Treated with Amoxicillin, Ceftiofur, or Cefquinome. Antimicrob. Agents Chemother. 52: 3612-3616 [Abstract] [Full Text]  
  • Lopez-Cerero, L., De Cueto, M., Saenz, C., Navarro, D., Velasco, C., Rodriguez-Bano, J., Pascual, A. (2008). Neonatal sepsis caused by a CTX-M-32-producing Escherichia coli isolate. J Med Microbiol 57: 1303-1305 [Abstract] [Full Text]  
  • Machado, E., Coque, T. M., Canton, R., Sousa, J. C., Peixe, L. (2008). Antibiotic resistance integrons and extended-spectrum {beta}-lactamases among Enterobacteriaceae isolates recovered from chickens and swine in Portugal. J Antimicrob Chemother 62: 296-302 [Abstract] [Full Text]  
  • Moreno, E., Andreu, A., Pigrau, C., Kuskowski, M. A., Johnson, J. R., Prats, G. (2008). Relationship between Escherichia coli Strains Causing Acute Cystitis in Women and the Fecal E. coli Population of the Host. J. Clin. Microbiol. 46: 2529-2534 [Abstract] [Full Text]  
  • Valverde, A., Grill, F., Coque, T. M., Pintado, V., Baquero, F., Canton, R., Cobo, J. (2008). High Rate of Intestinal Colonization with Extended-Spectrum-{beta}-Lactamase-Producing Organisms in Household Contacts of Infected Community Patients. J. Clin. Microbiol. 46: 2796-2799 [Abstract] [Full Text]  
  • Afset, J. E., Anderssen, E., Bruant, G., Harel, J., Wieler, L., Bergh, K. (2008). Phylogenetic Backgrounds and Virulence Profiles of Atypical Enteropathogenic Escherichia coli Strains from a Case-Control Study Using Multilocus Sequence Typing and DNA Microarray Analysis. J. Clin. Microbiol. 46: 2280-2290 [Abstract] [Full Text]  
  • Lavilla, S., Gonzalez-Lopez, J. J., Miro, E., Dominguez, A., Llagostera, M., Bartolome, R. M., Mirelis, B., Navarro, F., Prats, G. (2008). Dissemination of extended-spectrum {beta}-lactamase-producing bacteria: the food-borne outbreak lesson. J Antimicrob Chemother 61: 1244-1251 [Abstract] [Full Text]  
  • Johnson, T. J., Wannemuehler, Y. M., Nolan, L. K. (2008). Evolution of the iss Gene in Escherichia coli. Appl. Environ. Microbiol. 74: 2360-2369 [Abstract] [Full Text]  
  • Pradel, N., Bertin, Y., Martin, C., Livrelli, V. (2008). Molecular Analysis of Shiga Toxin-Producing Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome Patients and Dairy Samples in France. Appl. Environ. Microbiol. 74: 2118-2128 [Abstract] [Full Text]  
  • Colgan, R., Johnson, J. R., Kuskowski, M., Gupta, K. (2008). Risk Factors for Trimethoprim-Sulfamethoxazole Resistance in Patients with Acute Uncomplicated Cystitis. Antimicrob. Agents Chemother. 52: 846-851 [Abstract] [Full Text]  
  • Mammeri, H., Eb, F., Berkani, A., Nordmann, P. (2008). Molecular characterization of AmpC-producing Escherichia coli clinical isolates recovered in a French hospital. J Antimicrob Chemother 61: 498-503 [Abstract] [Full Text]  
  • Soto, S. M., Bosch, J., Jimenez de Anta, M. T., Vila, J. (2008). Comparative Study of Virulence Traits of Escherichia coli Clinical Isolates Causing Early and Late Neonatal Sepsis. J. Clin. Microbiol. 46: 1123-1125 [Abstract] [Full Text]  
  • Ziebell, K., Konczy, P., Yong, I., Frost, S., Mascarenhas, M., Kropinski, A. M., Whittam, T. S., Read, S. C., Karmali, M. A. (2008). Applicability of Phylogenetic Methods for Characterizing the Public Health Significance of Verocytotoxin-Producing Escherichia coli Strains. Appl. Environ. Microbiol. 74: 1671-1675 [Abstract] [Full Text]  
  • Bronowski, C., Smith, S. L., Yokota, K., Corkill, J. E., Martin, H. M., Campbell, B. J., Rhodes, J. M., Hart, C. A., Winstanley, C. (2008). A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli. Microbiology 154: 571-583 [Abstract] [Full Text]  
  • Johnson, J. R., Johnston, B., Clabots, C. R., Kuskowski, M. A., Roberts, E., DebRoy, C. (2008). Virulence Genotypes and Phylogenetic Background of Escherichia coli Serogroup O6 Isolates from Humans, Dogs, and Cats. J. Clin. Microbiol. 46: 417-422 [Abstract] [Full Text]  
  • Sabri, M., Caza, M., Proulx, J., Lymberopoulos, M. H., Bree, A., Moulin-Schouleur, M., Curtiss, R. III, Dozois, C. M. (2008). Contribution of the SitABCD, MntH, and FeoB Metal Transporters to the Virulence of Avian Pathogenic Escherichia coli O78 Strain {chi}7122. Infect. Immun. 76: 601-611 [Abstract] [Full Text]  
  • Zdziarski, J., Svanborg, C., Wullt, B., Hacker, J., Dobrindt, U. (2008). Molecular Basis of Commensalism in the Urinary Tract: Low Virulence or Virulence Attenuation?. Infect. Immun. 76: 695-703 [Abstract] [Full Text]  
  • Piatti, G., Mannini, A., Balistreri, M., Schito, A. M. (2008). Virulence Factors in Urinary Escherichia coli Strains: Phylogenetic Background and Quinolone and Fluoroquinolone Resistance. J. Clin. Microbiol. 46: 480-487 [Abstract] [Full Text]  
  • Subramanian, S., Roberts, C. L., Hart, C. A., Martin, H. M., Edwards, S. W., Rhodes, J. M., Campbell, B. J. (2008). Replication of Colonic Crohn's Disease Mucosal Escherichia coli Isolates within Macrophages and Their Susceptibility to Antibiotics. Antimicrob. Agents Chemother. 52: 427-434 [Abstract] [Full Text]  
  • Nicolas-Chanoine, M.-H., Blanco, J., Leflon-Guibout, V., Demarty, R., Alonso, M. P., Canica, M. M., Park, Y.-J., Lavigne, J.-P., Pitout, J., Johnson, J. R. (2008). Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15. J Antimicrob Chemother 61: 273-281 [Abstract] [Full Text]  
  • Karisik, E., Ellington, M. J., Livermore, D. M., Woodford, N. (2008). Virulence factors in Escherichia coli with CTX-M-15 and other extended-spectrum -lactamases in the UK. J Antimicrob Chemother 61: 54-58 [Abstract] [Full Text]  
  • Loukiadis, E., Nobe, R., Herold, S., Tramuta, C., Ogura, Y., Ooka, T., Morabito, S., Kerouredan, M., Brugere, H., Schmidt, H., Hayashi, T., Oswald, E. (2008). Distribution, Functional Expression, and Genetic Organization of Cif, a Phage-Encoded Type III-Secreted Effector from Enteropathogenic and Enterohemorrhagic Escherichia coli. J. Bacteriol. 190: 275-285 [Abstract] [Full Text]  
  • Carattoli, A., Garcia-Fernandez, A., Varesi, P., Fortini, D., Gerardi, S., Penni, A., Mancini, C., Giordano, A. (2008). Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum -Lactamases Isolated in Rome, Italy. J. Clin. Microbiol. 46: 103-108 [Abstract] [Full Text]  
  • Majalija, S., Segal, H., Ejobi, F., Elisha, B. G. (2008). Shiga Toxin Gene-Containing Escherichia coli from Cattle and Diarrheic Children in the Pastoral Systems of Southwestern Uganda. J. Clin. Microbiol. 46: 352-354 [Abstract] [Full Text]  
  • Gomez-Duarte, O. G., Chattopadhyay, S., Weissman, S. J., Giron, J. A., Kaper, J. B., Sokurenko, E. V. (2007). Genetic Diversity of the Gene Cluster Encoding Longus, a Type IV Pilus of Enterotoxigenic Escherichia coli. J. Bacteriol. 189: 9145-9149 [Abstract] [Full Text]  
  • Schierack, P., Walk, N., Reiter, K., Weyrauch, K. D., Wieler, L. H. (2007). Composition of intestinal Enterobacteriaceae populations of healthy domestic pigs. Microbiology 153: 3830-3837 [Abstract] [Full Text]  
  • Karami, N., Martner, A., Enne, V. I., Swerkersson, S., Adlerberth, I., Wold, A. E. (2007). Transfer of an ampicillin resistance gene between two Escherichia coli strains in the bowel microbiota of an infant treated with antibiotics. J Antimicrob Chemother 60: 1142-1145 [Abstract] [Full Text]  
  • Le Gall, T., Clermont, O., Gouriou, S., Picard, B., Nassif, X., Denamur, E., Tenaillon, O. (2007). Extraintestinal Virulence Is a Coincidental By-Product of Commensalism in B2 Phylogenetic Group Escherichia coli Strains. Mol Biol Evol 24: 2373-2384 [Abstract] [Full Text]  
  • Corvec, S., Prodhomme, A., Giraudeau, C., Dauvergne, S., Reynaud, A., Caroff, N. (2007). Most Escherichia coli strains overproducing chromosomal AmpC {beta}-lactamase belong to phylogenetic group A. J Antimicrob Chemother 60: 872-876 [Abstract] [Full Text]  
  • Jeziorowski, A., Gordon, D. M. (2007). Evolution of Microcin V and Colicin Ia Plasmids in Escherichia coli. J. Bacteriol. 189: 7045-7052 [Abstract] [Full Text]  
  • Moulin-Schouleur, M., Reperant, M., Laurent, S., Bree, A., Mignon-Grasteau, S., Germon, P., Rasschaert, D., Schouler, C. (2007). Extraintestinal Pathogenic Escherichia coli Strains of Avian and Human Origin: Link between Phylogenetic Relationships and Common Virulence Patterns. J. Clin. Microbiol. 45: 3366-3376 [Abstract] [Full Text]  
  • Walk, S. T., Mladonicky, J. M., Middleton, J. A., Heidt, A. J., Cunningham, J. R., Bartlett, P., Sato, K., Whittam, T. S. (2007). Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms. Appl. Environ. Microbiol. 73: 5982-5989 [Abstract] [Full Text]  
  • Ishii, S., Meyer, K. P., Sadowsky, M. J. (2007). Relationship between Phylogenetic Groups, Genotypic Clusters, and Virulence Gene Profiles of Escherichia coli Strains from Diverse Human and Animal Sources. Appl. Environ. Microbiol. 73: 5703-5710 [Abstract] [Full Text]  
  • Mammeri, H., Poirel, L., Nordmann, P. (2007). Extension of the hydrolysis spectrum of AmpC {beta}-lactamase of Escherichia coli due to amino acid insertion in the H-10 helix. J Antimicrob Chemother 60: 490-494 [Abstract] [Full Text]  
  • Pallecchi, L., Bartoloni, A., Fiorelli, C., Mantella, A., Di Maggio, T., Gamboa, H., Gotuzzo, E., Kronvall, G., Paradisi, F., Rossolini, G. M. (2007). Rapid Dissemination and Diversity of CTX-M Extended-Spectrum {beta}-Lactamase Genes in Commensal Escherichia coli Isolates from Healthy Children from Low-Resource Settings in Latin America. Antimicrob. Agents Chemother. 51: 2720-2725 [Abstract] [Full Text]  
  • Ihssen, J., Grasselli, E., Bassin, C., Francois, P., Piffaretti, J.-C., Koster, W., Schrenzel, J., Egli, T. (2007). Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Microbiology 153: 2052-2066 [Abstract] [Full Text]  
  • Ho, P. L., Poon, W. W. N., Loke, S. L., Leung, M. S. T., Chow, K. H., Wong, R. C. W., Yip, K. S., Lai, E. L., Tsang, K. W. T., on behalf of the COMBAT study group, (2007). Community emergence of CTX-M type extended-spectrum {beta}-lactamases among urinary Escherichia coli from women. J Antimicrob Chemother 60: 140-144 [Abstract] [Full Text]  
  • Boczek, L. A., Rice, E. W., Johnston, B., Johnson, J. R. (2007). Occurrence of Antibiotic-Resistant Uropathogenic Escherichia coli Clonal Group A in Wastewater Effluents. Appl. Environ. Microbiol. 73: 4180-4184 [Abstract] [Full Text]  

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