INTRODUCTION

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The food-borne pathogen Listeria monocytogenes represents a problem for the food industry because it is found ubiquitously in nature, it can grow at refrigeration temperatures, and it is capable of growth over a wide range of osmotic pressures (8, 9). Like many organisms, L. monocytogenes responds to hyperosmotic stress by accumulating osmotically active compounds, which are not inhibitory to enzymatic processes within the cell; these compounds are the so-called compatible solutes. The accumulation of these solutes, termed osmoadaptation, serves to counteract the outward flow of water, thereby maintaining cell turgor (6, 10, 19). In L. monocytogenes, the compatible solutes betaine and carnitine are also thought to play a role in cold adaptation. The rate of betaine accumulation is found to be maximal at about 10°C, and growth is stimulated almost twofold at 4°C when betaine is included in the growth medium (20). Carnitine accumulates to higher levels (three- to fourfold) at 4°C than at 30°C (20) and is transported at significant rates at 7°C (31).

The compounds used by bacteria as compatible solutes include amino acids, such as proline and glutamate; quaternary amines, such as betaine and carnitine; sugars (e.g., trehalose); and small peptides (e.g., N-acetyl-glutaminyl-glutamine amide and prolyl-glycyl-glycine). From among these, L. monocytogenes is known to derive osmoprotective effects from betaine (N,N,N-trimethylglycine [20]), carnitine (-hydroxy--[trimethyloammonio]butyrate [4]), and small proline-containing peptides (2). The transport of betaine and carnitine by L. monocytogenes has been studied in some detail. The extent of betaine accumulation is directly dependent on the osmolarity of the growth medium (20, 27, 28). The intracellular concentration of betaine increases approximately 20-fold when the external osmolarity is raised by 1.5 M with NaCl (20). The transport rate is osmotically activated and, at least in part, sodium driven (11). The transport of carnitine in L. monocytogenes is ATP dependent and is mediated by a high-affinity uptake system, with a K_m of 10 µM and a V_max of 48 nmol min¹ mg of protein¹ (31). This system appears to be specific for L-carnitine and the related compounds acetylcarnitine and butyrobetaine; the presence of either proline or betaine in excess has little effect on carnitine uptake (31). The accumulation of carnitine is influenced by the osmolarity of the medium, and uptake is subject to negative regulation by preaccumulated solute (32).

For other gram-positive organisms, such as Bacillus subtilis, Corynebacterium glutamicum, and Lactococcus lactis, a number of compatible solute transporters have now been well characterized. B. subtilis has at least five solute transporters (each given the designation Opu, for osmoprotectant uptake). OpuE is a single-component proline transporter and a member of the sodium/solute symporter family (34). OpuD is a single-component glycine betaine transporter (15). OpuA, OpuB, and OpuC are all closely related transporters which belong to the ATP binding cassette (ABC) superfamily of transporters and which can transport proline betaine and glycine betaine (OpuA); choline (OpuB); and ectoine, crotonobetaine, -butryobetaine, carnitine, choline-O-sulfate, choline, proline betaine, and glycine betaine (OpuC) (19). In C. glutamicum, BetP has been identified as the main glycine betaine transporter. It belongs to same family of transporters as OpuD from B. subtilis and CaiT (carnitine transport) and BetT (choline transport) from Escherichia coli (29). In a recent study, a glycine betaine transporter was identified for L. lactis and designated BusA (betaine uptake system). It is an ABC transporter which is closely related to the OpuA system from B. subtilis (26).

Despite several studies on the physiology of compatible solute uptake in L. monocytogenes, not until recently have genetic approaches led to the identification of two betaine transporters in this organism. BetL is a high-affinity secondary betaine transporter that is required for optimal growth in high-osmolarity medium (30). It is a member of a small family of transport proteins including BetT and CaiT from E. coli, BetP from C. glutamicum (29), and OpuD from B. subtilis (19). A second transporter, designated Gbu, was identified recently by screening of a mutant library for mutants defective in betaine-mediated osmoprotection (21). This transporter is encoded by three genes (gbuABC) which are closely related to the opuAA, opuAB, and opuAC genes of B. subtilis (18, 21). Recently, a membrane vesicle system was used to demonstrate that the Gbu transporter can be activated either by an osmotic gradient or by low temperatures (12). Gbu belongs to the ABC superfamily of transporters, which includes the ProU betaine transporter from E. coli. It is not yet clear whether the BetL and Gbu transporters represent the only betaine transporters in L. monocytogenes or whether one or more others remain unidentified.

No carnitine transporter has yet been identified at the genetic level for L. monocytogenes. Here we report the identification and characterization of an operon from L. monocytogenes encoding a new member of the ABC superfamily. We show that this operon is required for efficient growth under conditions of elevated osmolarity and demonstrate that a mutation in this operon leads to a defect in the ability to transport the solute L-carnitine.

	MATERIALS AND METHODS

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Bacterial strains and growth conditions. The wild-type L. monocytogenes serotype 1/2a strain EGD was used throughout. It was grown in brain heart infusion (BHI) broth (Difco), defined medium (DM) (2), or DM supplemented with 0.5% (wt/vol) type I peptone (Sigma) (DMP). Cultures were grown in 25 ml of medium (in 125-ml flasks) at 30°C with aeration. Cell growth was monitored spectrophotometrically (Ultrospec 4050; LKB, Biochrom) by measuring the optical density at 600 nm (OD₆₀₀) of 1-ml samples taken at suitable intervals during growth. The opuC::pAULA mutant was maintained on BHI agar plates with 2 µg of erythromycin ml¹. In liquid medium, erythromycin selection was not used, as it was found to reduce the growth rate. PCR was used to confirm that the opuC::pAULA integration was stable even in the absence of selection. The E. coli strain DH5 was used routinely for cloning experiments. It was cultured in Luria broth at 37°C. Strains were maintained long term at 80°C in 1-ml aliquots after the addition of 7% (vol/vol) dimethyl sulfoxide (Sigma) to overnight cultures.

Mutant construction. The opuCA gene was insertionally inactivated using the temperature-sensitive suicide vector pAULA. This 9.2-kb plasmid carries an erythromycin resistance marker, the pUC19 multicloning site, and a temperature-sensitive replication origin which enables chromosomal integration events to be selected at a nonpermissive temperature (7). Oligonucleotide primers directed against the opuCA gene were used to amplify a 400-bp region internal to the gene by PCR. The PCR primers (P121 [5'-TGACGGATCCACATCATGGCGGAAGATC-3'] and P117 [5'-TGACGGATCCGCTTCATCCATATCATGG-3']) used included at their 5' ends BamHI recognition sites (underlined) which facilitated the cloning of the PCR product into BamHI-digested pAULA. The resulting clone, designated pCOB26, was isolated from E. coli DH5 and used to electrotransform L. monocytogenes EGD. Chromosomal integration of pCOB26 was selected by repeated plating at 42°C with selection for erythromycin (2 µg ml¹) resistance as described previously (7). The integration of pCOB26 into the opuC locus in EGD was confirmed by PCR, and the resulting strain was designated EGD opuC::pAULA. The vector-specific For (position 20) and Rev primers (5'-TGTAAAACGACGGCCAGT-3' and 5'-CAGGAAACAGCTATGAC-3', respectively) were used to confirm the absence of independently replicating pCOB26 in the integrant strain. The PCR primers P125 and P114 (5'-CACACGTGCCACAAGTACG-3' and 5'-CACGAGTAACAATTCCGACAAG-3', respectively), which amplify the entire wild-type opuCA gene, were used to confirm the insertion of pAULA in the integrant strain (no PCR product was detected from the integrant strain). The primers P125 and For were used for further confirmation of this integration (a PCR product was detected only from the integrant strain).

Cloning and sequencing. A 4.3-kb HindIII fragment from L. monocytogenes ScottA (which was being investigated because a Tn917 insertion conferring acid sensitivity mapped to this region) was sequenced and found to carry two full open reading frames (ORFs) and a partial ORF spanning 2.3 kb at the 3' end of the fragment. These ORFs showed high levels of sequence similarity to the opuC and opuB operons of B. subtilis. The sequence obtained from the 4.3-kb clone was used to design PCR primers for the amplification of this region from L. monocytogenes EGD. The remainder of the operon was amplified by inverse PCR. Southern analysis of the opuC region in L. monocytogenes EGD indicated that the 3' end of the operon was carried on a 4.2-kb ClaI fragment (data not shown). Chromosomal DNA isolated from EGD was digested with ClaI, purified on a Wizzard column (Promega), and ligated with T4 DNA ligase (Roche). The ligated chromosomal DNA was then used as a template for PCR with oligonucleotide primers directed against the known opuC sequences. The 2.5-kb inverse PCR product obtained was sequenced by primer walking. The sequence was obtained from both strands using a BigDye Terminator cycle sequencing kit (PE Applied Biosystems). The DNA sequence was determined using a Perkin-Elmer Applied Biosystems 377 automated sequencer. The DNA sequence obtained was analyzed using DNASTAR Inc. software on a Power Macintosh computer.

Betaine and carnitine transport assays. The uptake of betaine and carnitine was measured using the method of Verheul et al. (31), modified as follows. Cells were grown overnight in DMP supplemented with 0.04% (wt/vol) glucose. Glucose (0.4% [wt/vol]) was added to the overnight culture, and growth was continued to an OD₆₀₀ of 0.4. This culture was then used to inoculate 100 ml of DMP (0.5% [vol/vol] inoculum). Cells were grown at 30°C with shaking to mid-exponential phase (OD₆₀₀ = 0.4). Cells were harvested by centrifugation for 10 min at 12,000 rpm and 4°C. The supernatant was removed, and cells were washed twice with ice-cold assay buffer (50 mM potassium phosphate [pH 6.9], 5 mM magnesium sulfate) containing chloramphenicol (50 µg ml¹). For studying uptake under high-osmolarity conditions, 0.5 M NaCl was added to the assay buffer. After being washed, cells were resuspended in the assay buffer to an OD₆₀₀ of 1. The transport assay was performed with 5 ml of cell suspension in the presence of 0.4% (wt/vol) glucose at 30°C with stirring. Radiolabeled L-[¹⁴C]betaine (ICN) or L-[³H]carnitine hydrochloride (Amersham) was added to a final concentration of 20 µM. Samples of 100 µl were removed, immediately filtered through glass microfiber filters (Whatman International Ltd.), and then washed twice with 2 ml of ice-cold buffer. Filters were dried, and the radioactivity was determined by scintillation counting. Samples (100 µl) were removed and transferred to filters, without filtering, and dried as standards for determination of betaine or carnitine specific activity. The total cell protein values used to calculate solute pools were 170 µg ml¹ per OD₆₀₀ unit at low osmolarity and 130 µg ml¹ per OD₆₀₀ unit at high osmolarity, as previously described (2).

Measurement of steady-state solute pools. Steady-state carnitine or betaine pools were measured using the method of Koo and Booth (22). Cells were grown in DMP as described above for the uptake assays. Cultures (25 ml) in DMP (0.4% [wt/vol] glucose) containing 200 µM betaine, carnitine, or choline were grown to an OD₆₀₀ of 0.2. A 2.5-ml aliquot was transferred to a sterile 25-ml test tube containing 10 nCi of L-[¹⁴C]betaine, 30 nCi of L-[³H]carnitine, or 600 nCi of [¹⁴C]choline (Amersham, Life Sciences). Incubation was continued at 30°C with shaking. When growth in the control flask reached an OD₆₀₀ of 0.4, the test tube was removed from the incubator. Three 0.5-ml samples were taken from the test tube, filtered through glass microfiber filters, and washed with 3 ml of DMP (lacking betaine, carnitine, or choline). The filters were dried, and the radioactivity was determined by scintillation counting. The remaining 1 ml of culture in the test tube was transferred to a cuvette, and the OD₆₀₀ was measured. Then, 50-µl samples were removed from the cuvette and transferred to filters, without filtering, and dried as standards for determination of betaine, carnitine, or choline specific activity. Final solute pool values were corrected for background radioactivity (i.e., radioactivity which was associated with the cells and which was not removed during the washing step) as follows. Cells were grown to an OD₆₀₀ of 0.4 as described above, and then 2.5 ml was permeabilized by the addition of 5% (vol/vol) butanol and incubation at 30°C for 30 min. Radiolabeled solute was added to the cells, and incubated was continued for a further 30 min. Samples were removed, filtered, and washed as described above for nonpermeabilized cells. Background radioactivity was measured by scintillation counting, and the values were used to correct the final solute pool calculations. The total cell protein values used to calculate uptake rates were 170 µg ml¹ per OD₆₀₀ unit at low osmolarity and 130 µg ml¹ per OD₆₀₀ unit at high osmolarity, as previously described (2).

Nucleotide sequence accession number. The GenBank accession number for the sequence described in this paper is AF249729.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Identification of the L. monocytogenes opuC operon. A newly identified ORF was being investigated in L. monocytogenes ScottA. This ORF was cloned on a 4.3-kb HindIII fragment, and the entire region was sequenced (C. P. O'Byrne, unpublished data). Downstream of the locus being investigated, two full ORFs and a partial ORF with high levels of sequence homology to the opuC and opuB operons of B. subtilis were identified. These ORFs were further investigated, as they represented ORFs for a newly identified putative solute transporter in L. monocytogenes. The opuC and opuB operons of B. subtilis encode ABC transporters capable of transporting a variety of compatible solutes into the cell in response to increased osmolarity (15, 16, 17, 19, 24). The full sequence of the corresponding operon in L. monocytogenes EGD was obtained by a combination of PCR, inverse PCR, and primer walking as described in Materials and Methods. EGD was the strain of choice for this analysis, as the genome sequence for this strain is scheduled for public release by September 2000 (see http://www.pasteur.fr/recherche/unites/gmp/Gmp_projects.html#lm). The ScottA and EGD sequences were 94.5% identical over the first two ORFs, although this figure may be an underestimate of their relatedness, as the ScottA sequence was obtained from one strand only in this region and therefore may have contained sequencing errors.

Sequence analysis. A 3,761-bp region of the EGD chromosome was sequenced (GenBank accession number AF249729), and four ORFs were identified (Fig. 1). ORF1, hereafter designated opuCA, is predicted to encode a hydrophilic protein of 397 amino acid residues (Fig. 2C) and with close sequence similarity (71% identical, 85% similar) to OpuCA from B. subtilis. In B. subtilis, OpuCA encodes an ATPase subunit associated with the OpuC ABC solute transporter. The Walker A and B motifs (35) and the linker peptide (5) are well conserved (Fig. 2A). The initiation codon of opuCA was identified as GTG, based on homology to the opuCA gene from B. subtilis and because this codon is preceded by a strong candidate Shine-Dalgarno sequence (16 to 10; 5'-ATGGAGG-3'). A potential SigB promoter was identified 58 bp upstream from the initiation codon of ORF1 (5'-GTTTAA-N14-GGGAAA-3'), based on a comparison with the promoter sequences of genes known to be SigB regulated in B. subtilis and L. monocytogenes. The 3' end of opuCA was found to be separated from the initiation codon of ORF2 by 6 bp, including the TAA stop codon.

View larger version (21K): [in this window] [in a new window]   FIG. 1.   Organization of the opuC operon in L. monocytogenes EGD depicted schematically. ORFs are shown as solid arrows. The numbers in parentheses above the ORFs indicate predicted sizes (in amino acid residues) of the protein products. A potential transcriptional terminator is indicated (TT). The potential SigB-dependent promoter is indicated by an angled arrow. The predicted amino acid sequence for each ORF was used to perform BLASTP searches (1) against the nonredundant databases (performed at http://www.ncbi.nlm.nih.gov/BLAST/). The eight proteins with the highest levels of sequence similarity (lowest E value [Expect value]) are listed beneath the corresponding ORF, followed in parentheses by the organism abbreviation and the percent identity. The organism abbreviations are as follows: Bs, B. subtilis; Mt, Mycobacterium tuberculosis; Ec, E. coli; Sp, Streptococcus pneumoniae; Dr, Deinococcus radiodurans; Af, Archaeoglobus fulgidus; Hp, Helicobacter pylori; Sc, Streptomyces coelicolor. Xxx, no assigned name.

View larger version (49K): [in this window] [in a new window]   FIG. 2.   Features of the OpuC protein subunits. (A) The first 200 amino acid residues of OpuCA from L. monocytogenes (Lm) were aligned by consensus with the corresponding N-terminal regions of OpuCa and OpuBA from B. subtilis (Bs), and identical residues are indicated by shading. Alignments were performed with a Clustal algorithm (14) using MegAlign software (DNASTAR Inc). The conserved "linker peptide" is overlined, and both Walker motifs (35) are overlined with hatched boxes. (B) The first 40 residues of the substrate binding protein (OpuCC) from L. monocytogenes (Lm) were aligned with the corresponding regions of OpuCC and OpuBC from B. subtilis (Bs). The predicted processing site for the pro-OpuCC lipoprotein is indicated with an arrowhead, and the conserved N-terminal cysteine residue is marked with an asterisk. (C) Kyte-Doolittle (23) hydrophobicity profiles for each of the four protein subunits of OpuC. The amino acid residue number is indicated above the plots. The profiles were obtained with a window of nine residues.

Growth characteristics of an opuC mutant. To test whether the opuC operon in L. monocytogenes played any role in osmoregulation, it was mutated by insertional inactivation of the opuCA gene as described in Materials and Methods. The mutant was found to grow normally in BHI broth (Fig. 3a). Supplementation of the medium with 4% NaCl led to a decrease in the growth rates of the wild type and the mutant. However, in the presence of added NaCl, the mutant was found to grow with a specific growth rate approximately half that of the wild type (0.41 and 0.90 h¹, respectively; Fig. 3a). In addition, the mutant formed very small colonies on BHI agar plates supplemented with 4% NaCl, whereas growth appeared normal on BHI agar plates without added NaCl (data not shown). Thus, inactivation of the opuC operon in L. monocytogenes led to reduced osmotolerance when cells were grown on BHI medium.

View larger version (17K): [in this window] [in a new window]   FIG. 3.   Growth of EGD (open symbols) and EGD opuCA::pAULA (solid symbols). (a) Cultures were grown in BHI in either the presence (squares) or the absence (circles) of 4% (wt/vol; 0.684 M) added NaCl. (b) Cultures were grown in DM without supplementation (triangles), with 0.5% (wt/vol) peptone (circles), or with 0.1% (wt/vol) Casamino Acids (squares).

Carnitine transport is abolished in an opuC mutant. The close sequence similarity between OpuC from B. subtilis and OpuC from L. monocytogenes prompted us to investigate whether the L. monocytogenes transporter was capable of transporting carnitine (in B. subtilis, OpuC is the sole uptake route for this compatible solute). Previous studies had indicated that carnitine transport was ATP dependent, suggesting the involvement of an ABC transporter. The kinetics of carnitine transport were studied using cells grown in DMP and assayed for solute uptake in potassium phosphate buffer, with or without added NaCl (0.5 M), in the presence of the protein synthesis inhibitor chloramphenicol (50 µg ml¹). Under these conditions, the parent strain was found to transport L-carnitine at a rate of approximately 10 nmol min¹ mg of cell protein¹, and this rate increased to approximately 50 nmol min¹ mg of cell protein¹ when uptake was assayed in the presence of added NaCl (Fig. 4a). In contrast, the opuC mutant strain showed no detectable carnitine uptake over the same time course, either with or without added NaCl. These data indicate that carnitine transport is stimulated by hyperosmotic shock and that both basal transport and osmotically stimulated transport are abolished in a strain lacking the OpuC transporter.

View larger version (18K): [in this window] [in a new window]   FIG. 4.   Compatible solute uptake in EGD (circles) or in the opuCA::pAULA mutant derivative (squares). Cultures were grown in DMP prior to the assay. The assay was performed with potassium phosphate buffer with (solid symbols) or without (open symbols) 0.5 M added NaCl. The assay was performed in the presence of the protein synthesis inhibitor chloramphenicol (50 µg ml1) and with 0.4% (wt/vol) added glucose. L-[3H]carnitine hydrochloride (a) or L-[14C]betaine (b) was added to a final concentration of 20 µM.

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Steady-state solute pools in an opuC mutant. Steady-state measurements of carnitine, betaine, and choline cytoplasmic pools were also obtained with cultures grown in DMP. Cultures were grown to mid-exponential phase in the presence or absence of 0.5 M NaCl, and the steady-state accumulation of radiolabeled solutes was measured by removing samples for filtration and scintillation counting as described in Materials and Methods. When carnitine accumulation was examined, the mutant was found to accumulate only a fraction of the levels accumulated by the parent strain (Fig. 5a). Wild-type cells grown in DMP without the addition of 0.5 M NaCl accumulated approximately four times as much carnitine as opuC mutant cells. The addition of NaCl (0.5 M) stimulated carnitine accumulation to approximately 400 nmol mg of cell protein¹ in the wild type, whereas the level increased only to 80 nmol mg of cell protein¹ in the mutant (Fig. 5a). Almost identical data were obtained when KCl was used as the osmolyte. Together, these data indicate that OpuC is required for the accumulation of high levels of carnitine under conditions of osmotic stress. The data also suggest the presence of an alternative route for carnitine uptake.

View larger version (29K): [in this window] [in a new window]   FIG. 5.   Compatible solute pools measured at steady state. Intracellular pools of carnitine (a), betaine (b), and choline (c) were measured as described in Materials and Methods. Cells of EGD (WT) or the opuCA::pAULA mutant (opuC) were grown in DMP either with (+) or without () 0.5 M NaCl or 0.5 M KCl. The error bars represent the standard deviation from the mean (n = 3).

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	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Physiological studies of L. monocytogenes have previously shown that the uptake of carnitine is ATP dependent, and transport was therefore predicted to occur via an ABC transporter (31). Here we have confirmed that an ABC transporter (designated OpuC, based on its close homology to the corresponding transporter in B. subtilis) belonging to the binding protein-dependent subgroup is the major carnitine uptake system in L. monocytogenes EGD. The ATP binding domains (which include Walker motifs [35]), which are the most characteristic feature of the ABC transporters (5, 13), are well conserved in the OpuCA protein (Fig. 2A). OpuC appears not to play a role in the accumulation of betaine or choline, compatible solutes that are chemically related to carnitine. This notion is consistent with the finding that betaine and choline have little inhibitory effect on this transporter, even when present at a 100-fold excess (31). It is interesting that in B. subtilis, OpuC does have a role, albeit a minor one, in betaine accumulation (16). The small degree of sequence divergence between the three related transporters (L. monocytogenes OpuC, B. subtilis OpuC, and B. subtilis OpuB) is therefore enough to account for considerable diversity in the substrate specificity. The determinant of substrate specificity in binding protein-dependent ABC transporters remains unclear, but the extracellular substrate binding subunit as well as the permease subunits are likely to play a role (13). In this respect, it is perhaps significant that among the four transporter subunits, the greatest divergence is seen in the substrate binding protein (Fig. 1).

The rate of carnitine transport in L. monocytogenes EGD is shown here to be osmotically stimulated (Fig. 4a). This stimulation is not dependent on increased levels of OpuC expression, as the uptake assays were performed in the presence of chloramphenicol. The presence of an osmotic gradient is therefore sufficient to stimulate the activity of the carnitine uptake system in the cell. This finding is in contrast to that of an earlier study showing that the initial rate of uptake of carnitine or betaine was independent of the assay medium osmolarity for cells cultured in DM (32). In that study, the authors also showed that osmotic stimulation was observed only when the cells were allowed to preaccumulate either betaine or carnitine, suggesting that the accumulation of either compatible solute could inhibit the activity of the transporter(s) and that this inhibition could be overcome by hyperosmotic shock. In the present study, cells were grown in DMP prior to the assay of carnitine uptake. It is possible that the preaccumulation of peptides (supplied in peptone), known to have an osmoprotective effect on L. monocytogenes (2), leads to inhibition of the carnitine transporter(s) and that this inhibition is reversed by hyperosmotic shock. In order to test this idea, we examined rates of carnitine transport by wild-type cells cultured in DM. The initial uptake rate was approximately 110 nmol mg¹ min¹ (10-fold faster than in DMP), and this rate was found to be independent of the assay medium osmolarity (data not shown). It seems likely, therefore, that the activity of the carnitine transporter(s) is inhibited by preaccumulated peptides or amino acids from the DMP growth medium and that this inhibition can be overcome, at least partially, by osmotic stimulation. The mechanisms underlying this regulation remain to be elucidated.

The identification of a putative SigB-dependent promoter upstream from the opuCA gene may indicate a role for this stress-inducible (3) sigma factor in the regulation of opuC expression. The sequence identified (at position 58) both closely resembles the consensus 10 and 35 boxes identified for other SigB-dependent promoters (3, 34) and shows a good match to the conserved spacing (14 ± 1 bp) between these elements (Fig. 6). Sleator et al. (30) have also identified a potential SigB-dependent promoter upstream of the betL gene in L. monocytogenes. It is interesting that two genes in B. subtilis that encode compatible solute transporters are also preceded by potential SigB-dependent promoters: opuD, which encodes a betaine transporter, and opuE, which encodes a proline transporter (Fig. 6) (34). It is noteworthy that in L. monocytogenes, a sigB mutant is impaired in its ability to use both betaine and carnitine as osmoprotectants. In addition, betaine transport is defective in a sigB mutant (3), although the transport of carnitine has not been studied directly with a strain lacking this sigma factor. Becker et al. (3) have also shown that the transcription of sigB is strongly induced by elevated osmolarity. Taken together, these observations suggest that opuC may be regulated at the transcriptional level by SigB. We are currently investigating this hypothesis.

View larger version (20K): [in this window] [in a new window]   FIG. 6.   Alignment of the putative opuC SigB promoter with known SigB promoters from L. monocytogenes and B. subtilis. The sequence identified upstream from opuCA in L. monocytogenes is shown aligned with other known or predicted SigB promoter sequences. Genes indicated with an asterisk have a potential SigB promoter which has not yet been confirmed experimentally. Position is given as the distance of the final base shown in the 10 box from the initiation codon. The alignment of promoters was adapted and updated from the literature (3, 34).

Here we have identified the opuC operon initially on the basis of sequence homology with opuC in B. subtilis. A recent study has also identified the opuC operon in L. monocytogenes by screening a bank of insertion mutants to isolate a carnitine uptake mutant (C. Hill, personal communication). In that study, a mutant was selected that was unable to grow on high-osmolarity medium supplemented with carnitine. The chromosomal region surrounding the plasmid insertion in this strains was sequenced and found to be almost identical to the sequences described here for the opuCA and opuCB genes. It is interesting to note that in that study, the mutant strain was also found to have defective growth in the DM used (Hill, personal communication). The reason for this growth defect remains unclear. It is possible that the OpuC transporter is also capable of transporting some component of DM (e.g., an amino acid) and that the inactivation of OpuC therefore leads to some auxotrophy. Provision of this component in an alternative form in the added peptone (e.g., a short peptide) may be sufficient to overcome this auxotrophy. Further studies are under way to address this possibility.

Given the complexity of solute transport in the related gram-positive organism B. subtilis, where at least five solute transporters are known to be involved in osmoregulation, it seems possible that other compatible solute transporters remain to be identified in L. monocytogenes. Recently, another solute-transporting ABC transporter was identified in L. monocytogenes: the Gbu betaine transporter, which is encoded by only three genes and which is related to the OpuA transporter in B. subtilis (21). Evidence also exists to indicate that peptide transport in L. monocytogenes, which is known to relieve hyperosmotic stress (2), is also dependent on an ATP-dependent transporter (33). There is substantial sequence similarity between OpuC and Gbu as well as between OpuC and OpuA, and it is possible that these transporters have evolved from a common ancestor, as seems likely for OpuC and OpuB in B. subtilis (17). It is possible that other ABC solute transporters in L. monocytogenes are responsible for transporting other solutes or accumulating solutes under other growth conditions. In this respect, it is interesting to note that even in the opuC mutant, there is still a detectable carnitine pool (approximately 20 nmol mg of cell protein¹ compared to approximately 70 nmol mg of cell protein¹ in the parent). Furthermore, this residual level of accumulation can be stimulated by the addition of NaCl (approximately three- to fourfold) (Fig. 5a). These data suggest that in L. monocytogenes, an alternative route for carnitine accumulation must exist. The availability of the L. monocytogenes genome sequence, which is due to be released shortly (see http://www.pasteur.fr/recherche/unites/gmp/Gmp_projects.html#lm), should allow other potential solute transporters to be identified and classified for this important food-borne pathogen.