INTRODUCTION

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Sorption of nonpolar contaminants by natural organic matter (NOM) is often characterized by a rapid step involving association with labile domains near the particle surface, followed by a slower step controlled by diffusion through intraparticle pores to hydrophobic regions (nonlabile domains) within the solid phase matrix (3, 17, 27). Retarded intraparticle diffusion of sorbed molecules results in significant desorption hysteresis (3, 7, 27) and is thought to be the rate-limiting step for biodegradation in soils and sediments. Many studies performed during the last decade support the hypothesis that rates of desorption or mass transfer from sorbed domains control rates of contaminant biodegradation (3, 5, 17, 27). Several studies support the conclusion that contaminants partitioned in the NOM phase are not directly available for degradation by microorganisms (4-6, 8, 13, 33). However, this general conclusion should be tempered by recent evidence that there is significant diversity in the abilities of microorganisms to degrade contaminants partitioned in or sorbed by NOM (4, 11, 31).

For example, two naphthalene-degrading isolates have been shown to exhibit significant variation in rates of degradation of sorbed naphthalene (11). One bacterial isolate, Alcaligenes sp. strain NP-Alk, did not enhance desorption of bound naphthalene, whereas Pseudomonas putida ATCC 17484 promoted naphthalene desorption and may have utilized bound naphthalene. Additional studies using naphthalene sorbed to XAD-2 (cross-linked polystyrene), a modified 2:1 layer silicate clay, and Tenax (2,6-diphenyl-p-phenylene oxide) confirmed that the P. putida strain had a greater ability to mineralize sorbed naphthalene than Alcaligenes sp. strain NP-Alk (6, 11). The possible physiological explanations for such diversity include variations in attachment to NOM surfaces (12, 16, 24), production of biosurfactants (14, 25), motility, rates of membrane transport, and the metabolic pathways used to mineralize phenanthrene (30). Although the majority of studies showing variations in responses to sorbed substrates have been done with cultivated microorganisms, they suggest that microenvironmental gradients in contaminant bioavailability play a role in the selection of specialized contaminant-degrading microorganisms. Many phenotypically different polycyclic aromatic hydrocarbon (PAH)-degrading isolates have been cultured in the laboratory by using classical enrichment approaches (2, 9, 18, 30) where the contaminant was provided as the sole carbon source, often in crystalline form at concentrations greater than the aqueous solubility limit. However, given the importance of sorption in controlling PAH bioavailability, it is questionable whether microbial isolates cultivated by using this approach are important in soil and sediment environments. Enrichment strategies in which contaminants are sorbed to organic solid phases may provide a more relevant selection environment and may eventually lead to a better understanding of what microorganisms and microbial processes are involved in the degradation of contaminants present primarily as a sorbed phase.

Model organic phases have been used in several recent studies to evaluate the effects of sorption and mass transfer rates on the microbial degradation of organic contaminants. Scow and Alexander (28) showed that the degradation of phenol and glutamate by a Pseudomonas sp. was controlled by diffusion from interparticle pore spaces in clay and polyacrylamide gel exclusion beads. Harms and Zehnder (13) used porous Teflon beads to study the degradation of sorbed 3-chlorodibenzofuran by a Sphingomonas sp. and found that attached cells degraded the bound chemical more rapidly than could be accounted for by the rates of desorption into the aqueous phase. Calvillo and Alexander (4) used polyacrylic porous resins to study the microbial degradation of sorbed biphenyl and found that biphenyl sorbed on polyacrylic beads was mineralized at a rate higher than the rate of abiotic desorption. However, until the recent work by Tang et al. (31), model solid phases had not been employed to cultivate microorganisms specifically adapted to low-bioavailability environments. In the study by Tang et al., a bacterium isolated by enrichment on phenanthrene sorbed to polyacrylic porous resin degraded sorbed phenanthrene more rapidly than did an isolate cultivated by using the traditional approach of providing contaminants in soluble or crystalline form. Although that study did not focus on microbial community structure, the results demonstrate the importance of enrichment strategies designed to more closely simulate the conditions in soil and/or sediment environments.

In this paper, we describe the use of model solid phases in enrichment experiments designed to evaluate degradation of a representative PAH (phenanthrene) and microbial selection for a range of contaminant bioavailabilities. In this study, we (i) determined the sorption-desorption characteristics of model solid phases used in enrichment cultures, (ii) evaluated the effects of phenanthrene sorption to model solid phases on the degradation kinetics in enrichment cultures by using two contaminated soils as initial inocula, and (iii) found that bacterial isolates enriched under reduced phenanthrene bioavailability conditions exhibit higher rates of phenanthrene degradation in a sorptive environment than isolates enriched in the presence of solid phase phenanthrene. In the accompanying paper (6a), we present the results of molecular analyses in which we examined the nature of microbial communities and isolates enriched under these conditions in order to corroborate that sorption-limited bioavailability plays an important role in the selection of contaminant-degrading microorganisms.

	MATERIALS AND METHODS

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Soils. Two soils from geographically distant sites were used as inocula for batch enrichments. One soil was obtained from an abandoned coal gasification site in Dover, Ohio, and the other was obtained from a wood treatment facility in Libby, Mont. (Table 1). Both soils exhibited significant levels of total petroleum hydrocarbons and PAHs (determined by Environmental Protection Agency methods 8440 and 8275A, respectively [22, 29]), suggesting that prior enrichment of PAH-degrading microorganisms had occurred. The potential of these soils to mineralize phenanthrene was verified by performing serum bottle radiorespirometry as described by Knaebel and Vestal (20). The soils were wetted to approximate field capacity by using sterile deionized distilled water (dH₂O), and then 0.1-ml aliquots of [9-¹⁴C]phenanthrene (total [9-¹⁴C]phenanthrene added = 0.007 mg kg¹) dissolved in acetone were added, followed by brief mixing. The [9-¹⁴C]phenanthrene (>98% pure) was obtained from Sigma Chemical Co. (St. Louis, Mo.) and had a specific activity of 59.5 mCi mmol¹. Evolution of ¹⁴CO₂, which was trapped on paper wicks saturated with 0.5 M NaOH and quantified by liquid scintillation counting, showed that indigenous microbial populations were capable of degrading approximately 45% of the freshly added phenanthrene (Table 1). Consequently, the soils contained active phenanthrene-degrading microbial populations.

Model organic phases. Model solid phases (Table 2) with different polarities, C/O ratios, and surface functional groups were selected to provide a range of phenanthrene sorption in enrichment cultures. The geometric surface areas of the solids were calculated based on mean particle size and ranged from 0.01 to 0.05 m² g¹. The surface area of the nonporous polymethylmethacrylate (PMMA) beads determined by using BET-N₂(g) isotherms (15) was 10-fold greater than the geometric surface area, indicating moderate surface roughness. The other solids used (Amberlite IRC-50 [AMB], Biobeads SM7, and Biobeads SM2) are porous resins that have considerably larger total surface areas. The surface area of AMB determined by using BET-N₂(g) isotherms while the solid was in the dry state may not reflect contributions from the internal surface area. Regardless, the internal surfaces of AMB, SM7, and SM2 are not accessible to microorganisms because the pore diameters are <0.01 µm. The AMB weak cation-exchange phase with carboxylic acid functionality was chosen as a model phase to represent the importance of carboxylic acid functional groups in humic substances.

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Phenanthrene degradation in the presence of model solid phases. Initial degradation experiments were conducted by using several phenanthrene-degrading isolates obtained from previous work in our laboratories. The purpose of this initial screening was to verify reduction in phenanthrene bioavailability in the presence of model solid phases and to demonstrate potential differences in the ability of microbial isolates to degrade phenanthrene in different sorptive environments. Individual isolates were obtained from several contaminated soils by using enrichment techniques (9) and phenanthrene spray-over plates (19). The additional soils utilized for bacterial isolation are not described in detail here but were either from abandoned coal gasification sites (Dover, Ohio) or creosote-contaminated sites that had previous hydrocarbon contamination (Gulf Breeze, Fla.). Phenanthrene degradation by nearly 30 isolates was confirmed by serum bottle radiorespirometry (20). Each isolate was grown in liquid culture (SSE) for 1 to 2 weeks by using solid phase phenanthrene as the sole C source. All cultures were brought to a constant optical density at 500 nm of 0.1 absorbance unit, and then 10-ml portions were inoculated into serum bottles in the absence (control) and presence of AMB, SM7, or SM2 solid phase containing sorbed [¹⁴C]phenanthrene. The initial cell densities, based on correlation of optical densities with CFU, ranged from 2 × 10⁷ to 9 × 10⁷ CFU ml¹.

Enrichment culture using model sorptive phases. The primary objectives of enrichment experiments were to determine the effects of phenanthrene sorption on phenanthrene bioavailability, as shown by reduced degradation rates, and to select for stable microbial populations that were potentially adapted to low-bioavailability environments. Duplicate batch enrichment cultures were initiated by using 1 g of soil (from Dover, Ohio, or Libby, Mont.) as inocula in 250-ml culture flasks containing 100 ml of SSE either in the presence or in the absence of model sorptive phases; this was followed by incubation at 25 ± 2°C with continuous aeration on an oscillating shaker (130 rpm). The amount of sorptive phase added to each enrichment culture (2 g of AMB, 1 g of SM2, or 1 g of SM7) was determined based on a target equivalent mass of 5.0 mg of phenanthrene (4.7 mg of C) per flask containing 0.022 µCi of [¹⁴C]phenanthrene. Controls without sorptive phases also contained 5.0 mg of phenanthrene per flask, which was added as an acetone solution; when the acetone evaporated, the solid phase phenanthrene precipitated (water solubility of phenanthrene = 1.8 mg liter¹ [22]). Phenanthrene degradation was monitored by frequent analysis (every 2 days) of ¹⁴CO₂, expressed as the percentage of added [¹⁴C]phenanthrene evolved over time. A 10-ml aliquot of each enrichment suspension (containing both solid and aqueous phases) was transferred to an identical fresh enrichment vessel just as the rate of phenanthrene degradation approached zero. The enrichment cultures were maintained for 5 to 12 transfers to determine the consistency of phenanthrene degradation rates and the stabilities of microbial populations selected under such conditions and to reduce any confounding effects of NOM or DNA added with the initial soil inoculum. At each transfer time, subsamples were removed and used to isolate phenanthrene-degrading microorganisms. Additional subsamples were frozen at 70°C for use in molecular analyses of microbial communities (6a).

Isolation of phenanthrene-degrading bacteria. Samples from enrichment cultures were used to isolate pure cultures of microorganisms, which were characterized for their abilities to degrade phenanthrene. Samples were taken from all enrichment cultures that were actively degrading phenanthrene, 10-fold serial dilutions in sterile 0.85% NaCl were prepared, and 100-µl portions of each dilution were spread on SSE plates or on yeast extract-peptone-glucose (YEPG) medium (1, 9). In some cases, AMB and SM7 beads were harvested by centrifugation, washed with SSE, and then treated in a sterile tissue homogenizer with SSE at the maximum speed. Dilutions of the supernatants were spread on agar plates so that bacteria attached to the bead surfaces could potentially form colonies on the agar medium. Phenanthrene was sprayed onto the agar plate surfaces, resulting in an opaque layer (19). Morphologically distinct colonies that were obtained with higher dilutions and formed clearing zones were considered potential phenanthrene-mineralizing organisms and were purified further by passages on either YEPG or SSE agar plates. The purity of >40 strains was checked microscopically and by molecular analysis by using denaturing gradient gel electrophoresis (DGGE) (6a). Bacterial isolates growing either on SSE agar plates or on YEPG agar plates were examined for their ability to degrade [¹⁴C]phenanthrene by using serum bottle radiorespirometry as described above. The phenanthrene-degrading isolates were associated primarily with three DGGE bands that were the predominant bands produced by the different enrichment cultures (6a). Phenanthrene degradation rates in the presence and in the absence of sorptive phases were evaluated in greater detail with three isolates that produced the dominant DGGE bands observed with SM7 enrichment (isolate SM7.6.1), control (isolate C4.7), and sand enrichment (isolate S2.1) cultures. Each isolate was pregrown in SSE by using solid-phase phenanthrene as the sole C source for 1 to 2 weeks. The optical density at 500 nm of each culture was adjusted to a constant value of 0.12 absorbance unit, and then 10-ml portions were inoculated into 250-ml culture flasks containing 90 ml of fresh SSE and 5 mg of phenanthrene (0.022 µCi of [¹⁴C]phenanthrene) as described above for control and sand, AMB, and SM7 enrichment cultures. The initial cell densities, based on correlation of optical density values with numbers of CFU, ranged from 1 × 10⁷ to 2 × 10⁷ CFU ml¹. Triplicate flasks kept under constant aeration were monitored for evolution of ¹⁴CO₂, which was used as a measure of phenanthrene degradation over time.

	RESULTS

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Model solid-phase properties: sorption and desorption. Sorption isotherms describing the partitioning of phenanthrene for each model solid phase were highly linear over a range of equilibrium phenanthrene concentrations (Fig. 1) and were used to calculate sorption coefficients (log K_D), which ranged from 2.3 liters kg¹ for PMMA to 4.2 liters kg¹ for the nonpolar SM2 phase (Table 2). The log K_D values for soils vary depending on the organic C content but often range from 2.5 to 3.5 liters kg¹ (23). The sand used in these experiments showed no appreciable sorption of phenanthrene (data not shown) and provided a nonsorptive control surface for microbial attachment. The results of sorption experiments suggest that the model solid phases which we used were suitable for achieving a range of phenanthrene bioavailability in enrichment experiments designed to select for microorganisms specifically adapted to low-bioavailability conditions. Preliminary experiments using PMMA as a model sorptive phase showed little reduction in phenanthrene degradation rate relative to controls when solid-phase phenanthrene was used. These results were consistent with the fact that the nonporous PMMA solid used in these experiments exhibited a lower K_D value than other model sorptive phases and the fact that the majority of phenanthrene bound by PMMA was not subject to significant limitations in mass transfer to degrading microorganisms, perhaps due to the lack of microporosity. Consequently, for the purpose of focusing on sorptive phases exhibiting reduced phenanthrene bioavailability, PMMA was not used in any further experiments described here.

View larger version (20K): [in this window] [in a new window]   FIG. 1.   Isotherms showing the sorption of phenanthrene by model organic solids, including PMMA, AMB (a porous weak cation exchanger with COOH functionality), SM7 (porous acrylic ester beads), and SM2 (porous divinyl benzene beads). The isotherm slopes correspond to calculated log KD values of 2.3, 3.0, 3.5, and 4.2 liters kg1 for PMMA, AMB, SM7, and SM2, respectively. Error bars indicate standard deviations for both x and y axis variables; where error bars are absent, they fall within the symbols.

View larger version (25K): [in this window] [in a new window]   FIG. 2.   Cumulative desorption of phenanthrene from preloaded model solids in three different extracting solutions: SSE, 1% Tween 80, and dH2O-methanol (1:1, vol/vol). The value for each time point represents desorption into fresh extracting solution. Error bars represent standard deviations; where error bars are absent, they fall within the symbols.

Phenanthrene degradation: initial characterization of enrichment conditions. The phenanthrene degradation experiments conducted with known phenanthrene-degrading bacterial isolates produced two important results. First, the maximum phenanthrene degradation rates and extents of degradation for nearly all of the isolates decreased in the following order: control  sand > AMB > SM7 > SM2. The degradation rates were highest in control vessels containing no sorptive phase and were nearly zero in the presence of SM2 (Fig. 3). This order is consistent with the results of sorption and desorption experiments, in which phenanthrene sorption rates increased and desorption rates decreased as the nonpolarity of the sorptive phase increased.

View larger version (31K): [in this window] [in a new window]   FIG. 3.   Phenanthrene degradation in the absence (control) and presence of sorbing solid phases by two bacterial isolates (GB.35, DC.61) obtained from soils by using phenanthrene spray-over plates. Control, no sorbed-phase phenanthrene present; AMB, phenanthrene sorbed to AMB; SM7, phenanthrene sorbed to SM7; SM2, phenanthrene sorbed to SM2.

Phenanthrene degradation in enrichment cultures. We performed enrichment experiments to simultaneously evaluate phenanthrene degradation kinetics and microbial community structure for a range of phenanthrene bioavailabilities. Our primary goal was to identify microorganisms selected in the enrichment cultures by using both cultivation-dependent and cultivation-independent methods (6a). Consequently, repeated transfers to fresh media were made in an effort to (i) minimize carryover of DNA and NOM associated with the initial soil inoculum and (ii) develop steady-state microbial assemblages exhibiting consistent phenanthrene degradation kinetics over time. Phenanthrene degradation rates in initial enrichment cultures (1% Dover soil inoculum) followed trends similar to those observed in individual microbial isolate experiments, where rates of degradation were inversely correlated with the phenanthrene sorption coefficients of the solids used in the enrichment cultures (Fig. 4). After subsequent transfers into fresh enrichment media, the preparations continued to exhibit consistent trends in degradation rates as a function of phenanthrene bioavailability (i.e., the order of the degradation rates was as follows: control  sand > AMB > SM7). Lag phases of 2 to 10 days were observed in the initial enrichment cultures containing AMB and SM7 when the Dover soil was used, but lag phases were generally nonexistent after subsequent transfers (Fig. 4), suggesting that these enrichment cultures selected for stable phenanthrene-degrading microbial assemblages. In the SM2 enrichment cultures, less than 5% of the phenanthrene added was degraded during the initial enrichment period, and less than 1% was degraded after subsequent transfers. These enrichment culture experiments were not continued due to insufficient phenanthrene degradation and due to a lack of sufficient biomass to permit molecular analysis of microbial populations.

View larger version (26K): [in this window] [in a new window]   FIG. 4.   Degradation of phenanthrene obtained with a Dover soil inoculum in batch enrichment cultures grown in the absence (control) and in the presence of sand, AMB, SM7, and SM2 sorptive phases. Results are shown for selected transfers and indicate the development of consistent patterns for the phenanthrene degradation rate as a function of time and as a function of solid-phase polarity. Error bars represent one standard deviation.

View larger version (25K): [in this window] [in a new window]   FIG. 5.   Degradation of phenanthrene obtained with a Libby soil inoculum in batch enrichment cultures grown in the absence (control) and presence of different phenanthrene sorptive phases. Results are shown for selected transfers and indicate the development of consistent patterns for the phenanthrene degradation rate as a function of time and as a function of solid-phase polarity. Error bars represent one standard deviation.

Phenanthrene degradation by isolates obtained from enrichment cultures with different bioavailabilities. More than 40 phenanthrene-degrading bacterial isolates were cultivated from the model enrichment cultures in an effort to identify important species present in all treatments. A subset of these isolates representing two of the primary populations associated with control and SM7 enrichment cultures (6a) was used to test the hypothesis that organisms selected under sorptive conditions (such as SM7 cultures) may differ in the ability to degrade sorbed-phase phenanthrene from organisms isolated under nonsorptive conditions. A comparison of representative isolates obtained from control (strain C4.7), sand (strain S2.1), and SM7 (strain SM7.6.1) enrichment cultures (Fig. 6) showed that isolates obtained from nonsorptive enrichment cultures degraded phenanthrene more slowly in the presence of SM7 than did isolates obtained from SM7 enrichment cultures. The maximum phenanthrene degradation rates in control enrichment cultures containing solid-phase phenanthrene were 8.8 µmol day¹ for isolate C4.7, 3.2 µmol day¹ for isolate S2.1, and 2.4 µmol day¹ for isolate SM7.6.1 (Fig. 6). These results show that isolates SM7.6.1 and S2.1 exhibited slower growth kinetics in the absence of AMB or SM7 than isolate C4.7. In the presence of the SM7 sorptive phase, the maximum phenanthrene degradation rates declined for all isolates. However, the reductions in degradation rates caused by reduced phenanthrene bioavailability varied considerably among isolates. This was best illustrated by normalizing for each isolate the maximum phenanthrene degradation rate in the presence of SM7 to that in the control environment. When this was done, the comparison should have factored out simple differences in microbial growth rates among isolates when no constraints on phenanthrene bioavailability were present. Sorption of phenanthrene to SM7 reduced the degradation rate by a factor of 2 for isolate SM7.6.1. In contrast, the sorptive phase reduced the rates of phenanthrene degradation approximately 10- to 15-fold for isolates C4.7 and S2.1. These results indicate that sorption of phenanthrene by organic phases constitutes an important environmental constraint for certain microorganisms, providing a basis for possible selection of different species in heterogeneous soil and sediment microenvironments. Although we have not examined the underlying mechanisms responsible for the differences among the isolates, we hypothesize that isolates such as SM7.6.1 are better adapted to sorptive environments than isolates C4.7 and S2.1.

View larger version (29K): [in this window] [in a new window]   FIG. 6.   Phenanthrene degradation in the absence (control) and presence of sand, AMB, and SM7 solid phases by Dover soil isolates obtained from either SM7 (SM7.6.1), control (C4.7), or sand (S2.1) enrichment cultures. Error bars represent one standard deviation.

	DISCUSSION

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Past research focusing on the effects of sorption on degradation of organic contaminants has generally been conducted by using microorganisms isolated under nonsorptive enrichment conditions (4, 10, 11, 26, 30, 33). It has often been concluded that a sorbed contaminant is not directly available for microbial degradation and that desorption into the aqueous phase is necessary for microbial uptake and subsequent degradation. It is thought that in such situations the contaminant desorption rate is the rate-limiting step for microbial degradation. However, recent work suggests that microorganisms vary in their abilities to degrade substrates bound to NOM or nonpolar solid phases (4, 10, 31). Consequently, conclusions concerning rates of microbial utilization of a sorbed substrate are likely dependent on the specific microbial isolate. Moreover, enrichments using sorptive phases to select for microorganisms specifically adapted to low-bioavailability conditions may be useful for identifying species that are important in soil and sediment environments and for elucidating mechanisms important for accessing sorbed-phase contaminants.

In the present work, we have shown that phenanthrene-degrading microbial isolates obtained by using canonical enrichment approaches (i.e., phenanthrene spray-over plates [19]) vary in their abilities to degrade phenanthrene sorbed by organic solid phases intended to simulate NOM. Some isolates obtained by using nonsorbed phenanthrene as the sole carbon source did not degrade appreciable amounts of phenanthrene sorbed to AMB (a weak cation-exchange phase with carboxylic acid functionality) or SM7 (acrylic ester functionality). For isolates capable of degrading phenanthrene in the presence of sorbing phases, degradation rates were generally inversely related to phenanthrene sorption. These results are consistent with those reported by others (4, 11, 13, 33) showing that sorption of nonpolar compounds to model solid phases reduced the degradation rates. Recently, Tang et al. (31) utilized sorptive environments to select microorganisms with enhanced abilities to mineralize sorbed PAHs.

In this paper and the accompanying paper (6a) we describe enrichment experiments designed to evaluate the relationships among phenanthrene sorption, phenanthrene degradation, and microbial selection. Phenanthrene degradation rates were monitored under four different enrichment conditions for numerous transfers following initial inoculation with two hydrocarbon-contaminated soils. Microbial populations selected in these enrichment cultures exhibited consistent patterns of phenanthrene degradation kinetics after several transfers to fresh media. An inverse relationship between the phenanthrene degradation rate and phenanthrene sorption to model solid phases was observed in enrichment cultures prepared with both contaminated soils (Fig. 4 and 5). In contrast to the degradation rates in the presence of SM2, the degradation rates in the presence of AMB and SM7 were much higher than would have been predicted from the rates of phenanthrene desorption in the absence of microorganisms (compare the percentage desorbed as shown in Fig. 2 to the amount mineralized as shown in Fig. 4 and 5). Weissenfels et al. (33) observed no degradation of anthracene sorbed on a resin similar to SM2 (XAD-2) by an acclimated mixed bacterial culture known to degrade several PAHs. Potential mechanisms of microbially facilitated desorption in SM7 and AMB environments include solubilization of the contaminant via production of biosurfactants and development of steep concentration gradients between the solid phase and interfacial phenanthrene (10, 12, 13, 32). It has also been suggested that microorganisms capable of attachment exhibit higher rates of degradation of a sorbed substrate (10, 12, 13). An examination of selected solid phases by scanning electron microscopy confirmed the presence of attached microorganisms in both AMB and SM7 enrichment cultures, suggesting that at least one member of each enriched culture was present on bead surfaces. However, further work will be necessary to evaluate the mechanisms responsible for the different degradation behaviors of isolates obtained from enrichment cultures in the presence and in the absence of sorptive phases and to determine whether other factors, such as surface characteristics of the solid phases, may play a role in the selection of phenanthrene-degrading microorganisms.

A subset of microbial isolates obtained from the enrichment cultures was used in experiments to compare the rates of phenanthrene degradation by individual isolates for the same range of phenanthrene bioavailabilities. We observed that one of the primary isolate types (6a) that originated from the SM7 enrichment cultures (SM7.6.1) degraded phenanthrene more rapidly in the presence of SM7 than isolates obtained from control enrichment cultures containing no sorptive phase (C4.7, S2.1). Our results are consistent with those of Tang et al. (31) showing that sorption of phenanthrene has an important effect on selection of microbial species with greater adaptation to low-bioavailability environments. In addition, our work shows that the range of phenanthrene bioavailabilities obtained by using solid phases with different sorptive properties provides a useful approach for determining the effects of contaminant sorption on microbial selection and for cultivating and characterizing microorganisms that may have more relevance in natural environments. Furthermore, in the accompanying paper (6a) we describe molecular analyses that were used to corroborate the finding that different microbial assemblages were obtained from enrichment cultures with different degrees of phenanthrene sorption.