Applied and Environmental Microbiology, August 2000, p. 3125-3133, Vol. 66, No. 8
Biology Department, Woods Hole Oceanographic
Institution, Woods Hole, Massachusetts 025431;
Institut für Chemie und Biologie des Meeres (ICBM),
Universität Oldenburg, 26111 Oldenburg,
Germany2; Netherlands Institute for Sea
Research (NIOZ), 1790 AB den Burg, The
Netherlands3; Department of Geosciences,
Princeton University, Princeton, New Jersey
085444; and FB 2 und UFT, Abt. Marine
Mikrobiologie, Universität Bremen, D-29359 Bremen,
Germany5
Received 9 February 2000/Accepted 24 April 2000
Species diversity, phylogenetic affiliations, and environmental
occurrence patterns of thiosulfate-oxidizing marine bacteria were
investigated by using new isolates from serially diluted continental
slope and deep-sea abyssal plain sediments collected off the coast of
New England and strains cultured previously from Galapagos hydrothermal
vent samples. The most frequently obtained new isolates, mostly from
103- and 104-fold dilutions of the continental
slope sediment, oxidized thiosulfate to sulfate and fell into a
distinct phylogenetic cluster of marine alpha-Proteobacteria. Phylogenetically and physiologically,
these sediment strains resembled the sulfate-producing thiosulfate
oxidizers from the Galapagos hydrothermal vents while showing
habitat-related differences in growth temperature, rate and extent of
thiosulfate utilization, and carbon substrate patterns. The abyssal
deep-sea sediments yielded predominantly base-producing
thiosulfate-oxidizing isolates related to Antarctic marine
Psychroflexus species and other cold-water marine strains
of the Cytophaga-Flavobacterium-Bacteroides phylum, in
addition to gamma-proteobacterial isolates of the genera Pseudoalteromonas and Halomonas-Deleya.
Bacterial thiosulfate oxidation is found in a wide phylogenetic
spectrum of Flavobacteria and Proteobacteria.
Recent microbial surveys of marine
offshore and deep-sea sediments have revealed considerable bacterial
biodiversity in this extreme marine environment; cultivated isolates
from deep-sea trench sediments include members of the actinomycetes, of
the low guanine-plus-cytosine gram-positive bacteria, and of alpha- and
gamma-Proteobacteria (66, 67). Heterotrophic,
aerobic, or denitrifying deep-sea isolates, mostly barophiles, from the Atlantic and Pacific Oceans belong to the gamma-proteobacterial genera
Colwellia, Moritella, and
Shewanella (14). Anoxic deep sediment layers
have yielded nitrate reducers, fermentative bacteria, and sulfate
reducers (46). Isolations from deep-sea hydrothermal vents
frequently focused on the microbial populations of the sulfur cycle.
Reduced sulfur species of hydrothermal origin favor abundant microbial
populations of mesophilic sulfur oxidizers, including autotrophic
Thiomicrospira and Thiobacillus species (17,
30, 54, 78) as well as heterotrophic sulfur-oxidizing strains (18, 54).
The bacterial communities of hydrothermal vent sites and of
nonhydrothermal water columns and sediments have at least one significant bacterial type in common, the loosely defined group of the
heterotrophic sulfur-oxidizing bacteria (18, 69). These heterotrophic bacteria can oxidize reduced sulfur compounds, but do not
depend on this reaction for growth. Two groups with different pH
responses can be distinguished, the acid-producing and the base-producing thiosulfate oxidizers (54, 69). In both
cases, the pH change is generally in the range of 0.5 to 1.5 pH units (54). Some base-producing strains studied in detail oxidized thiosulfate as an auxiliary electron donor to tetrathionate; this allowed them to use a greater portion of available organic carbon for
biosynthesis rather than for respiration (68, 71). No CO2 fixation could be demonstrated for base-producing
strains. The acid-producing hydrothermal vent strain TB66 showed
increased cell yields in thiosulfate-amended cultures (29).
The acid-producing strain NF18 showed thiosulfate-stimulated
CO2 assimilation during growth on mineral medium
(54); in this regard, it resembled the
chemolithotrophic genera Thiobacillus or
Thiomicrospira which oxidize thiosulfate completely to
sulfate (54).
Due to their ecophysiological flexibility, thiosulfate-oxidizing
heterotrophs are very widespread in the marine environment and have
been isolated from a wide range of marine habitats, i.e., coastal
waters of Vineyard Sound, the open North Atlantic, and the chemoclines
of the Black Sea and the Cariaco Basin (31, 60, 69, 70).
These isolations have rarely been linked to quantification of these
bacteria and comparisons of their population density in different
habitats. With the exception of incomplete sequences for three alpha-
and gamma-proteobacterial thiosulfate oxidizers (strains NF18, AG33,
and NF13) from the Galapagos hydrothermal vents (35),
phylogenetic identifications of cultured marine isolates are, for the
most part, missing.
Culture characteristics and phylogenetic affiliation of newly isolated
thiosulfate-oxidizing bacteria from two North Atlantic sediment
locations, and of previously isolated Galapagos hydrothermal vent
strains (29, 54), were investigated in order to identify possible habitat-related common denominators as well as recurring differences in physiology and phylogenetic identity of these bacteria.
Sampling and sediment characterization.
A 50- by 50-cm box
corer was used to retrieve sediment samples of red clay from the North
Atlantic abyssal plain at a depth of 4,500 m (37°29'N, 68°50'W) and
to retrieve olive-colored silty sediment from the continental slope off
New England at a depth of 1,500 m (39°50'N, 70°14'W). A box core
with undisturbed sediment surface was used to obtain subcores (15-cm
diameter, 40-cm depth) for further analysis. The cores were kept at
4°C, and sediment surfaces remained covered with ca. 0.5 cm of
overlying water. The extent of the oxic zone, which defined the
sampling scheme, was measured with oxygen electrodes on the ship and in
the shore laboratory after 36 h, with identical results
(52). Pore water was extracted within 48 h under
N2 by using a small-scale pore water squeezer
(53). Sulfide was measured with the methylene blue method
immediately after pore water extraction (10). Organic carbon
content and organic carbon (Corg)/total nitrogen
(Ntotal) ratios were determined from acid-dried sediment
with an EA 1108 Fisons Elemental Analyzer (Fisons Instruments, Inc.,
Beverly, Mass.).
Microbiological media.
Thiosulfate medium for
sulfur-oxidizing bacteria contained (per liter): 25 g of NaCl,
1.0 g of (NH4)2SO4, 1.5 g
of MgSO4 × 7H2O, 0.42 g of
KH2PO4, 0.3 g of KCl, 0.2 g of
NaHCO3, 0.29 g of CaCl2 × 2H2O, 1.58 g of
Na2S2O3, 1 ml of SL-8 trace element solution (5), 1 ml of vitamin solution (2 mg
liter
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Diversity of Thiosulfate-Oxidizing Bacteria from
Marine Sediments and Hydrothermal Vents
ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
1 each of biotin and folic acid; 5 mg
liter1 each of niacine, panthothenate, lipoic acid,
p-aminobenzoic acid, thiamine, riboflavin, pyridoxine, and
cobalamin) (3). The NaHCO3 addition to the salt
base imitates the natural carbonate-buffering system of seawater and
was instrumental in isolating new sulfate-reducing bacteria
(77) and sulfur-oxidizing bacteria (74). Two
milliliters of 0.5% phenol red solution was added per liter of medium
as a pH indicator. The pH was adjusted to 7.3, and the medium was
filter sterilized with 0.2-µm-pore-size filters. Liquid medium was
used for determining the most probable numbers (MPNs). Oxygen
limitation in the MPNs, which could prevent complete oxidation of
thiosulfate to sulfate (73), was ruled out by capping the
MPN vials with very loosely fitting plastic caps that allowed gas
exchange. Thiosulfate medium was used as liquid MPN medium or for 1.5%
agar plates. Sulfate-free thiosulfate medium was prepared with 0.4 g of NH4Cl and 1.24 g of MgCl2 × 6H2O instead of the corresponding sulfates; this medium was
used for quantification of sulfate produced by thiosulfate oxidation.
Negative control plates did not contain thiosulfate, but otherwise
contained the same salts, vitamins, trace elements, and pH indicator.
Quantifications and isolations. MPN counts of sulfate-reducing and thiosulfate-oxidizing bacteria were done in parallel; sulfate-reducing bacteria are a source of reduced sulfur compounds in the sediment. MPN dilutions were performed in triplicate as previously described (1) with defined media for sulfate-reducing and for thiosulfate-oxidizing bacteria; the series included six 10-fold dilution steps. MPN dilution series for both media were inoculated in parallel with 0.1 ml of oxic surface sediments from the 0- to 1-cm layer at both sampling sites and with 0.1 ml of sediment from the microoxic 1- to 2-cm layer of the slope sediment. This definition of the oxic sediment layers was based on oxygen profiles of the freshly harvested sediment cores, prior to core sectioning and MPN inoculation. The MPNs for sulfate-reducing bacteria were judged positive if sulfide production, checked by the CuSO4 test (77), coincided with visible cell growth.
The thiosulfate MPN had to be evaluated differently. Changes of pH were used as a preliminary indicator of thiosulfate-oxidizing activity. Bacterial growth never reached the level of visible turbidity; the indicators of microbial activity were pH changes and microscopically visible cells obtained by centrifuging 100- to 500-µl portions of the medium. Over the course of 2 months, different bacterial populations developed in the liquid samples that increased or lowered the pH. An interpretation of all pH changes as positive would have been simplistic and of no value to account for the obviously diverse bacterial populations in these samples. Therefore, pure cultures were isolated individually from the highest dilutions which showed distinct pH changes. This approach allows the identification and cultivation of numerically dominant, but slow-growing or fastidious, bacteria that would be overgrown in enrichment culture (32). In order to maximize the diversity of the isolates, fully aerobic and microoxic-nitrate-reducing incubation regimens were chosen. Two 100-µl samples of each MPN sample were streaked on two thiosulfate agar plates. The first thiosulfate plate of each pair was incubated under fully aerobic conditions at 15°C. The second thiosulfate plate contained, in addition, 0.2 g of KNO3 per liter. All nitrate plates were incubated at 15°C in a gas-tight incubation jar which had been flushed for 20 min with nitrogen gas, but without removing small amounts of residual oxygen (for example, the small amount dissolved in the agar plates) by reducing agents. Strains obtained in this way are marked with an asterisk (e.g., alpha-proteobacterial strains DI4*, EI1*, and DIII4*). Individual colonies of all strains were obtained by restreaking on agar plates and continued incubation. The plates were checked for pH changes and thiosulfate oxidation to sulfate. Instead of generalized MPN values, the dilution step from which each thiosulfate-oxidizing isolate was obtained is given subsequently. The characteristic pH changes of these isolates during growth on thiosulfate plates were not observed on parallel thiosulfate-free control plates.Origin of vent strains. The hydrothermal vent strains were isolated from different locations and sample materials at the Galapagos hydrothermal vents in 1979 (E. Ruby, personal communication) and were enriched on thiosulfate mineral media made with aged seawater and adjusted to pH 7.2 or 8 (54). Strain AG33 was obtained from the filtrate of a water sample at the Garden of Eden site characterized by abundant vent fauna. Strain TB66 was obtained from an unspecified Galapagos vent sample. Strain NF18 was isolated from Beggiatoa mat material and was enriched in thiosulfate medium where NH4Cl was omitted to select for nitrogen-fixing isolates (54). The aged seawater used for these thiosulfate mineral media contained small amounts of organic substrates, similar to the agar-solidified thiosulfate medium used for the North Atlantic sediment isolates.
Phenotypic testing. Heterotrophic growth on different carbon sources was tested on thiosulfate-free agar plates with 10 mM carbon source. Growth was compared to negative controls without additional carbon source. The plates were incubated for a month at 15°C and were checked at intervals of 1 to 4 days. Growth at different temperatures (4, 15, 24, 37, and 42°C) was tested on marine 2216 heterotrophic agar plates (Difco, Detroit, Mich.); the plates were checked for growth for 10 days, but the results did not change after 3 or 4 days. The ability to use nitrate as electron acceptor was checked by stab inoculation of marine 2216 soft agar tubes (0.3% wt/vol) containing 0.1% (wt/vol) KNO3. Nitrite production was checked with alpha-naphthylamine; denitrification was considered to have occurred when gas bubbles in the agar coincided with the disappearance of nitrate according to the zinc powder test (22).
Sulfate production from thiosulfate was measured by streaking strains on sulfate-free agar slants containing 10 mM thiosulfate and phenol red as pH indicator. This strategy became necessary since attempts to grow the strains in liquid mineral medium turned out to be extremely time-consuming. pH changes indicative of thiosulfate-oxidizing activity took several months, while they took 1 to 2 weeks on agar slants. Traces of organic carbon compounds in the agar facilitated growth and accelerated the thiosulfate oxidation process. Since organic supplements in high concentration sometimes inhibited thiosulfate oxidation (see Results), oligotrophic, unsupplemented agar slants were a reasonable working compromise. Agar slants also allowed observation of the developing bacterial lawn on the agar surface in order to verify growth, whereas liquid media did not show increased turbidity or accumulation of a visible bacterial pellet. Each strain was streaked out in triplicate. The pH decrease was monitored by following the color change of the pH indicator until no further pH change occurred. Agar portions of 1 ml were excised from directly underneath the culture surface and were mixed overnight with 2 ml of sterile deionized water; the resulting 1:3 dilution of the medium was centrifuged to spin down and remove the agar before ion chromatography. After the samples for sulfate determination had been excised, the final pH of the agar slants was checked with a pH electrode. The isolate DIII1c, which did not change the pH and did not produce sulfate from thiosulfate, was included as a negative control. Sulfate was detected by high-pressure liquid chromatography with indirect UV detection as described previously (51). A 50-µl volume of the culture medium was injected by using a Rheodyne valve (sample loop volume, 25 µl). Separation of anions was performed on a Polyspher IC AN-1 anion-exchange column (Merck). Indirect UV was measured at 254 nm in a Merck/Hitachi-L-4250-UV/VIS detector. An L-6210 intelligent pump connected to the UV/VIS detector was used. Flow rate for the isocratic eluent (1.5 mM phthalic acid, 1.38 mM Tris-hydroxymethyl-aminomethane, 300 mM boric acid, pH 4.0) was 1.3 ml min1. Calibrations were carried out several times
(n = 3) with dilutions of a MgSO4 standard
(312 µM SO42) in the range of 0 to 312 µM
sulfate. The retention time for sulfate under these conditions was
11.55 min. All samples were diluted 1:25 with double-distilled water
before injection into the high-pressure liquid chromatography. All
sulfate determinations were done in triplicate for each culture tested.
DGGE.
The isolates were divided into similarity groups by
phenotypic comparisons and by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments. DGGE separates double-stranded DNA fragments of identical length but different primary
sequence, based on different melting domain structure and denaturation
stability in a denaturing gradient. DGGE fragments of identical gel
position most likely share identical sequences, although different DGGE
fragments can match in their positions by chance. Pure cultures were
analyzed by DGGE of PCR-amplified 16S rRNA gene fragments, using PCR
primers GM5 (with guanosine-cytosine clamp) and 907R (41).
DGGE was performed with a Bio-Rad Protean II system. A 20 to 70%
denaturing gradient was used for all experiments. A value of 100%
corresponds to 7 M urea and 40% (vol/vol) formamide (41).
Electrophoresis was continued for 20 h at 100 V and 60°C. After
electrophoresis, the gels were stained in aqueous ethidium bromide
solution (0.5 µg liter1) and photographed on a UV (302 nm) transillumination table with a Cybertech CS1 digital camera
(Cybertech, Berlin, Germany).
Sequencing and phylogenetic analysis.
Representative strains
were phylogenetically identified by 16S rRNA sequencing. With pure
culture DNA as template, 16S rRNA genes were PCR-amplified as described
(42) and were sequenced directly by using the Taq
Dyedeoxy Terminator Cycle Sequencing Kit (Applied Biosystems, Foster
City, Calif.). The sequence reactions were electrophoresed on an
Applied Biosystems 373S DNA sequencer. Sequences were aligned by using
the alignment editor SeqPup (23). Jukes-Cantor distance
trees were calculated and bootstrapped with 200 resamplings by using
the PHYLIP software package 3.57c (19). For parsimony
bootstrap, 200 resamplings were performed with Paup 3.1.1 (65). The partially sequenced thiosulfate-oxidizing
hydrothermal vent isolates AG33 and NF18 (35) were
resequenced and included in the phylogenetic analysis. The
thiosulfate-oxidizing hydrothermal vent strain TB66 (29) was
newly sequenced and included in the phylogeny. Table
1 lists all isolates obtained in this
study and GenBank numbers for sequenced strains. Near-complete 16S rDNA sequences used for the tree were retrieved from GenBank for the following organisms: Roseobacter denitrificans, M59063;
Roseobacter litoralis, X78312; Sulfitobacter
pontiacus, Y13155; Sargasso Sea isolate S34, U87407;
Prionitis lanceolata gall symbiont, U37762;
Octadecabacter arcticus, U73725; Octadecabacter antarcticus, U14583; Ruegeria gelatinovora, D88523;
Marinosulfonomonas methylotropha, U62894; Roseovarius
tolerans Y11551; Roseobacter algicola, X78315;
Roseobacter gallaeciensis, Y13244; Sargasso Sea strain NFR,
L15345; Antarctobacter heliothermus, Y11552; Sagittula
stellata, U58356; Ruegeria atlantica, D88526; Silicibacter lacuscaerulensis, U77644; Paracoccus
aminophilus, D32239; Paracoccus denitrificans, Y17511;
Sargasso Sea clone SAR83, M63810; Pseudoalteromonas
tetraodonis, X82139; Pseudoalteromonas haloplanktis,
X67024; Alteromonas macleodii, X82145; Mariana trench
sequence no. 1, D87345; Halomonas meridiana, M93356; Deleya aquamarina, M93352; Halovibrio variabilis,
M93357; Deleya halophila, M93353; Halomonas
elongata, M93355; Psychroflexus torquis, AF001365;
Psychroflexus gondwanense, M92278; Flavobacterium salegens, M92279; Cytophaga latercula, D12665;
Cytophaga lytica, M62796; Cytophaga marinoflava,
D12668; Psychroserpens burtonensis, U62913;
Gelidibacter algens, U62914; and Flexibacter maritimus, D14023.
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RESULTS |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Acid-producing thiosulfate-oxidizing bacteria. The cultivation survey yielded different microbial populations from the continental slope and abyssal sediments; in general, heterotrophic, pH-increasing, and pH-decreasing thiosulfate-oxidizing bacterial isolates showed different occurrence patterns with respect to the sediment samples of their origin and the dilution from which they were isolated.
The most frequently and consistently found group of thiosulfate-oxidizing bacteria were heterotrophic isolates from 103- and 104-fold dilutions of the slope sediment which acidified the medium moderately by ca. 0.5 to 1.0 pH units (Table 2). The isolate AIII3 constitutes an exception; it was obtained from a 103-fold dilution of the abyssal plain sediment, and it acidified the medium by 1.7 to 1.8 U (Table 2). These acid-producing thiosulfate oxidizers showed phylogenetic and physiological similarities to the previously isolated hydrothermal vent thiosulfate oxidizers, and were therefore studied in more detail than the base-producing strains. Sulfate production from thiosulfate was a consistent feature of these isolates, although the extent of sulfate production varied considerably from strain to strain. Over 20 days of aerobic incubation at 15°C, they produced ca. 2 to 4.6 mM sulfate, corresponding to oxidation of approximately 10 to 23% of the original 10 mM thiosulfate in the medium to sulfate (Table 2). Parallel incubations on unsupplemented and 10 mM pyruvate-supplemented agar slants show different thiosulfate oxidation patterns for these sediment strains; in some cases, pyruvate inhibits thiosulfate oxidation and sulfate production, indicating that these strains prefer organic substrates over inorganic electron donors such as thiosulfate. On the other hand, the isolates DIII4* and EI1* show greatly increased thiosulfate oxidation after pyruvate supplementation, indicating that these organisms use thiosulfate and organic substrates in parallel (Table 2). The sediment isolates grew aerobically or by nitrate reduction. Strains DIII4* and EI1* reduced nitrate completely to nitrogen gas and grew much better in nitrate-reducing agar shake cultures than on plates incubated aerobically. The strains DII3 and DIII3 grew as aerobes, but could also reduce nitrate to nitrite (Table 2). All sediment strains grew at 15 or 24°C, but not at 37 or 42°C (Table 2). The substrate spectra of the sediment strains show common denominators (Table 3): organic acids and amino acids such as acetate, propionate, butyrate, glutamate, and proline and citric acid cycle intermediates can serve as sole carbon sources, but sugars generally cannot. The only exception observed so far is the C5-monomer of the common plant and bacterial cell wall component xylan, xylose, which supports growth of strains AIII3, DI4, DII3, and DIII3.
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Base-producing thiosulfate-oxidizing bacteria. Most base-producing strains were isolated from 103- and 104-fold dilutions in the abyssal sediment. In contrast to the alpha-proteobacterial strains from the slope sediment, they did not belong to a single phylogenetic branch, but were affiliated with three different groups: the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum, the gamma-proteobacterial genus Pseudoalteromonas, and the closely intertwined gamma-proteobacterial genera Deleya and Halomonas. After growth on sulfate-free thiosulfate agar slants for 20 days under the same conditions as the acid-producing thiosulfate oxidizers, sulfate production was found for representatives of the CFB group and of Deleya-Halomonas, but not for Pseudoalteromonas.
CFB phylum.
Five isolates of the CFB phylum were obtained.
Three strains came from 104 dilutions and one came from a
103 dilution of the surface 1 cm of the abyssal sediment
(AIII4, AIII4*, AII4*, and AII3, respectively); a single isolate was
obtained from a 101 dilution of the 1- to 2-cm layer of
the slope sediment (EI1*). These isolates were conspicuous by slightly
increasing the pH of thiosulfate plates by approximately 0.5 pH units.
Strain AII3 was tested for thiosulfate oxidation and produced 2.5 mM
sulfate. All isolates were strictly aerobic and did not reduce nitrate. They showed intense yellow coloration, in contrast to all other strains
in this study, which were unpigmented. With the exception of abyssal
strain AIII4, all isolates showed the same DGGE band (Fig. 1, lanes 12 to 16). The 16S rDNA of a representative of this common DGGE type,
strain AII3, and the deviating strain AIII4 were sequenced. The
isolates were closely related to each other (100% bootstrap) and
belonged to a well-supported clade (92% distance and 86% parsimony
bootstrap) of marine CFB species mostly from cold-water habitats,
including the halophilic, antarctic species F. salegens,
P. gondwanense, and P. torquis (4, 16)
(Fig. 2).
Pseudoalteromonas. Five Pseudoalteromonas isolates (AI4*, AIII3*, AI3*, AI3, and AII2) were obtained, in this order, from one 104-, three 103-, and one 102-fold dilution of the surface 1-cm layer of abyssal sediment. All Pseudoalteromonas isolates showed identical DGGE patterns (Fig. 1, lanes 17 to 21). The 16S rDNA sequence of isolate AIII3* was determined and was found to be nearly identical to the 16S rDNA sequence of P. tetraodonis (21) (Fig. 2). The Pseudoalteromonas isolates raised the pH of thiosulfate plates by 0.7 to 0.8 pH units. Strain AIII3* was tested for thiosulfate oxidation; sulfate was not found after growth on thiosulfate agar slants. The Pseudoalteromonas strains were strictly aerobic, did not reduce nitrate, and formed unpigmented colonies.
Deleya-Halomonas and others.
In contrast to the
CFB and Pseudoalteromonas isolates from high dilutions of
the abyssal sediment, the base-producing isolates (DII1a, DII1b, DIII1,
and DII2*) were obtained from 101 and 102
dilutions of the slope sediment surface layer. These isolates differed
from all others by rapidly increasing the pH of the agar plates from
ca. 7.5 to more than 8.5, leading to a deep-purple coloration of the
phenol red indicator, and by rapid growth and colony formation within 1 or 2 days on thiosulfate and heterotrophic plates. The isolates DII1a
and DII2* were tested for thiosulfate oxidation and formed ca. 1.8 to 2 mM sulfate. The 16S rRNA sequence of strain DII1a was nearly identical
to the sequences of D. aquamarina and of H. meridiana (Fig. 2), two closely related species of the phenotypically and phylogenetically intertwined genera
Deleya and Halomonas which could be combined into
a single genus (15). Variability was found with respect to
nitrate reduction and DGGE position (Fig. 1, lanes 22 to 25), the
strains DII1a and DII1b grew anaerobically and reduced nitrate to
nitrite, and strain DII2* did not reduce nitrate and showed a different
DGGE position.
Sediment characteristics and sulfate reduction.
The two sample
sediments differed substantially in organic carbon and C/N ratios. The
slope sediment showed an organic carbon content of 1.7 to 1.8% organic
C per dry weight of sediment and a Corg/Ntotal
ratio of 9 to 9.5 for the upper-5-centimeter layers; the abyssal
sediment had an organic carbon content of 0.58 to 0.62% and a
Corg/Ntotal ratio of 6.5 to 7.5. After
microbial consumption of easily degradable organic matter, clay-bound
residues with low Corg/Ntotal ratios are left
behind and not further degraded by bacteria (39). In
comparison to the slope sediment, the abyssal sediment was more
depleted of microbially utilizable substrates. The organic carbon and
C/N ratios of the samples were characteristic of continental slope and
abyssal sediments in the North Atlantic (12, 58) and
indicate that the samples were representative for these sediment
environments. The activity of the reductive sulfur cycle in these
sediments was evaluated by MPN counts of sulfate-reducing bacteria and
by determinations of pore water sulfate and sulfide concentrations.
Sulfate-reducing bacteria (SRB) were found in the surface layers of the
continental slope sediment samples that also yielded the majority of
the alpha-proteobacterial isolates. The surface and the 1- to 2-cm
sediment layers yielded approximately 100 cultivable SRBs
ml1 with lactate, and the 1- to 2-cm layer yielded, in
addition, approximately 20 cultivable SRB ml1 with
acetate. The MPN counts were performed in the surface layers, since
marine sediments often show the highest sulfate reducer densities
around the oxycline near the sediment surface (at ca. 1-cm depth in the
slope sediment). Here, high numbers of SRB coincide with rapid
oxidation, reduction, and disproportion of thiosulfate, sometimes
performed by the SRBs themselves (32). MPN counts in the
abyssal sediment were unsuccessful; the population density of
cultivable SRB was too low for MPN quantification. Free sulfide in the
pore water and dark-gray sediment layers indicative of sulfide
precipitates or pyrite were absent from both sediments. Pore water
sulfate remained at seawater concentration (ca. 28 mM) and showed no
sign of depletion in both cores, with bioturbation being absent. These
data indicate low sulfate reduction activity and a correspondingly low
supply of reduced sulfur species for thiosulfate-oxidizing bacteria.
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DISCUSSION |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Hydrothermal vent and sediment strains. The acid-producing alpha-proteobacterial thiosulfate oxidizers from hydrothermal vents and sediments are phylogenetically related and physiologically similar; their differences appear to be in degree rather than in principle and show the imprint of the two environments. Under low-nutrient conditions on unsupplemented agar slants as well as under high carbohydrate load, the hydrothermal vent strains oxidized more thiosulfate to sulfate than most of the sediment strains. Visible acidification by thiosulfate oxidation as well as heterotrophic growth and colony formation could be seen after 2 days for the hydrothermal vent strains, but took 1 to 2 weeks for the sediment strains under equal conditions. The vent strains showed a larger substrate spectrum than the sediment strains, which included easily degradable sugars. Thus, the heterotrophic thiosulfate oxidizers can make versatile and quick use of the reduced sulfur and carbon sources in the hydrothermal vent environment: thiosulfate is supplied by reduced vent fluids or by chemical oxidation of metal sulfides (56). Dissolved organic carbon and particulate organic carbon levels are several hundred times more concentrated in water of vent areas, compared to normal deep-sea bottom water (8, 11). The abundant bacterial biomass growing on chimneys and sediments is also a likely source of organic nutrients (3, 28). This metabolic menu differs from the diagenetically altered and nutrient-depleted organic matter that reaches deep-sea sediments. For example, sinking organic matter retained only ca. 1% of its original content of carbohydrates, amino acids, and lipids and consisted mostly of uncharacterized refractory compounds after passage through a 1,000-m water column (76). Therefore, the limited substrate range of the thiosulfate oxidizers in the slope sediment, compared to the hydrothermal vent strains, most likely reflects the lack of undegraded, fresh substrates within the sedimentary organic carbon pool.
The reduced thiosulfate oxidation capacity of the sediment alpha-proteobacterial strains is consistent with low sulfate reduction activity and a low supply of reduced sulfur species. The microbial degradation of organic carbon from primary production and terrestrial input during sedimentation through the deep-water column severely limits organic substrate availability and, subsequently, the activity and density of anaerobic sulfate-reducing bacteria in the deep sea (2, 9).Possible occurrence patterns. The alpha-proteobacterial thiosulfate oxidizer populations in slope and abyssal sediments could follow nonrandom occurrence patterns. Generally, cloning and sequencing of environmental 16S rRNA genes showed the ubiquitous presence of marine alpha-Proteobacteria in the water column of all oceans (20, 40, 64) and in marine snow (49). They are especially abundant in coastal seawater. In a cloning survey, they accounted for a fifth of all bacterial clones obtained from the continental shelf near Cape Hatteras (48); in estuaries with near-marine salinity, they contributed 13 to 28% of the total amount of bacterial DNA (25). An important ecological role of several members of the marine alpha group is the decomposition of organic sulfur compounds produced mostly by eukaryotic phytoplankton (38) and salt marsh plants (45). Marine alpha group bacteria oxidize dimethylsulfoniopropionate and dimethylsulfide (26, 36) or are capable of degradation of complex plant-produced polymers such as lignin or humic substances (24). The thiosulfate oxidizers of the slope sediment could be derived from these coastal populations, although their location in outer continental slope sediments rules out active participation in coastal or pelagic cycling of organosulfur compounds (36, 74).
Members of the CFB phylum occur ubiquitously in the marine environment and are generally associated with the degradation of complex organic substrates (50). They represent one of the most frequently isolated bacterial groups from marine sediments, due to the relative ease of their isolation and their occurrence in high numbers (37, 43). The phylogenetic affiliation of the deep-sea isolates with a cluster of isolates from cold saline and marine environments (7) suggests that several related CFB species have specifically adapted to the conditions of the cold oceans, including the polar seas and the deep sea. As a caveat, an autochthonous deep-sea origin of the abyssal CFB strains is not proven; 16S rDNA sequences of CFB members have been obtained from marine snow particles (13, 49) which could carry these natural inoculates to the bottom sediments (44). A similar pattern could apply to the Pseudoalteromonas isolates. Previous marine surveys have identified Pseudoalteromonas as a frequently isolated bacterial genus in Antarctic sea ice and seawater (6) and in coastal North Atlantic waters (43). Molecular surveys of marine water columns have recovered Pseudoalteromonas 16S rDNA sequences, although not as frequently as marine alpha-proteobacterial or CFB sequences (40). The Deleya-Halomonas isolates were obtained from low dilutions of the slope sediment only (101 and 102
cells ml1), indicating that their fast growth and easy
isolation from diverse seawater, saline lake water, salted food, and
marine biomass samples (34, 75) do not necessarily
correspond to high cell densities in situ.
Phylogenetic diversity. The 16S rRNA-based identification of cultured heterotrophic marine thiosulfate oxidizers puts a sharper focus on this group, with evolutionary and ecological implications. For example, the sediment isolates of the marine alpha-proteobacterial cluster, the most frequently isolated strains in this study, show an unexpected phylogenetic and physiological link with the microbial populations of hydrothermal vents. The hydrothermal vent thiosulfate oxidizers of the marine alpha-proteobacterial cluster, NF18, AG33, and TB66 (29, 54) are the evolutionary cousins of the widespread marine sediment or water column bacteria of the same group. This marine alpha-proteobacterial group is ecologically and physiologically highly diversified. In addition to the sediment and hydrothermal vent bacteria described here, this phylogenetic group also includes polar sea ice bacteria of the genus Octadecabacter (27); marine sediment-dwelling bacteria of the genus Ruegeria (55, 72); facultative phototrophs, i.e., R. litoralis and R. denitrificans (57); and organosulfur-oxidizing marine heterotrophs (24, 36). Without exception, all acid- and sulfate-producing thiosulfate oxidizers isolated in this study fall into this cluster. This strongly suggests a phylogenetic basis for the thiosulfate-oxidizing phenotype within this group.
The marine thiosulfate-oxidizing, base-producing species include members of the CFB phylum, and species of the gamma-proteobacterial genera Pseudoalteromonas and Halomonas- Deleya. A phenotypic study identified alkaliphilic, denitrifying, thiosulfate-oxidizing, tetrathionate-producing bacteria as members of the genus Deleya (61). In a phenotypic and 16S rDNA study, anaerobic tetrathionate-producing thiosulfate oxidizers were identified as members of Pseudomonas stutzeri genomovars (63). The default designation Pseudomonas (59, 68, 71) for these organisms does not reflect their actual diversity. In short, the ability to oxidize thiosulfate and probably also other reduced sulfur compounds is widespread among different genera of heterotrophic alpha- and gamma-Proteobacteria. The frequent isolation of thiosulfate oxidizers from chemoclines of stratified marine water columns such as the Black Sea (31, 60) and the Cariaco Basin (70) and the high thiosulfate-oxidizing activity recently reported from coastal organic-matter-rich Baltic Sea sediments (47) demonstrate that these bacteria are ubiquitous catalysts in the marine sulfur cycle at oxic-anoxic interfaces.| |
ACKNOWLEDGMENTS |
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We thank the crew of the RV Oceanus and George Hampson for their expert handling of the boxcorer and their determination to obtain good samples. We also thank all cruise participants, in particular Christian Knoblauch for moral and practical support, last but not least against encroaching seasickness.
Andreas Teske was supported by DFG postdoctoral fellowship 262-1/1 and a subsequent WHOI postdoctoral fellowship.
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FOOTNOTES |
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* Corresponding author. Mailing address: Woods Hole Oceanographic Institution, Biology Department/Redfield Laboratory, Woods Hole, MA 02543. Phone: (508) 289-2305. Fax: (508) 457-2134. E-mail: ateske{at}whoi.edu.
Contribution no. 10187 of the Woods Hole Oceanographic Institution.
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REFERENCES |
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Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Mikucki, J. A., Priscu, J. C.
(2007). Bacterial Diversity Associated with Blood Falls, a Subglacial Outflow from the Taylor Glacier, Antarctica. Appl. Environ. Microbiol.
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Perreault, N. N., Andersen, D. T., Pollard, W. H., Greer, C. W., Whyte, L. G.
(2007). Characterization of the Prokaryotic Diversity in Cold Saline Perennial Springs of the Canadian High Arctic. Appl. Environ. Microbiol.
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Sattley, W. M., Madigan, M. T.
(2006). Isolation, Characterization, and Ecology of Cold-Active, Chemolithotrophic, Sulfur-Oxidizing Bacteria from Perennially Ice-Covered Lake Fryxell, Antarctica. Appl. Environ. Microbiol.
72: 5562-5568
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Dai, X., Wang, B.-J., Yang, Q.-X., Jiao, N.-Z., Liu, S.-J.
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Penn, K., Wu, D., Eisen, J. A., Ward, N.
(2006). Characterization of Bacterial Communities Associated with Deep-Sea Corals on Gulf of Alaska Seamounts. Appl. Environ. Microbiol.
72: 1680-1683
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Cytryn, E., van Rijn, J., Schramm, A., Gieseke, A., de Beer, D., Minz, D.
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Knittel, K., Kuever, J., Meyerdierks, A., Meinke, R., Amann, R., Brinkhoff, T.
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Mills, H. J., Martinez, R. J., Story, S., Sobecky, P. A.
(2004). Identification of Members of the Metabolically Active Microbial Populations Associated with Beggiatoa Species Mat Communities from Gulf of Mexico Cold-Seep Sediments. Appl. Environ. Microbiol.
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Ogier, J.-C., Lafarge, V., Girard, V., Rault, A., Maladen, V., Gruss, A., Leveau, J.-Y., Delacroix-Buchet, A.
(2004). Molecular Fingerprinting of Dairy Microbial Ecosystems by Use of Temporal Temperature and Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol.
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Brinkhoff, T., Bach, G., Heidorn, T., Liang, L., Schlingloff, A., Simon, M.
(2004). Antibiotic Production by a Roseobacter Clade-Affiliated Species from the German Wadden Sea and Its Antagonistic Effects on Indigenous Isolates. Appl. Environ. Microbiol.
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Brettar, I., Christen, R., Hofle, M. G.
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Bruns, A., Cypionka, H., Overmann, J.
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68: 3978-3987
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Sass, A. M., Sass, H., Coolen, M. J. L., Cypionka, H., Overmann, J.
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