Erratum for Bhavsar et al., Appl. Environ. Microbiol. 67 (1) 403-410.
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Applied and Environmental Microbiology, November 2001, p. 5349-5349, Vol. 67, No. 11
0099-2240/01   DOI:
Copyright © 2001, American Society for Microbiology. All rights reserved.

ERRATUM

Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes in Bacillus subtilis: Conditional Complementation of a Teichoic Acid Mutant

Amit P. Bhavsar, Xumei Zhao, and Eric D. Brown

Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5

Volume 67, no. 1, p. 403-410, 2001. Page 405, Fig. 1: the plasmid map depicted in Fig. 1a is not correct; the orientation of the plasmid backbone is the reverse of that shown in the published article. Figure 1 should read as shown below.


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  		FIG. 1.   Map of plasmid pSWEET-bgaB. (a) Significant features of the xylose-based expression system. Plasmid pSWEET-bgaB is a derivative of pDG364 (3), which allows integration into the B. subtilis chromosome at amyE via double recombination and selection with CHL (10 µg/ml). The plasmid also has an E. coli origin of replication and ampicillin resistance cassette (50 µg/ml) for routine cloning steps. On the outside of the plasmid map restriction sites of interest are highlighted, including two PstI sites for convenient plasmid linearization, a PacI site (eight-base recognization sequence TTAATTAA) upstream of bgaB, and a polylinker downstream of bgaB (HindIII is not unique). (b) Close-up of key elements of the xylose expression system (not to scale). Shown are xylR, encoding the xylose repressor; the xyl intergenic region, including promoters for xylR (PxylR), xylA (PxylA), and xyl operator sequences (xylO); translationally truncated xylA (first 58 nucleotides followed by an in-frame TAA), including an optimized CRE in xylA (see Materials and Methods and reference 18); PacI 5' cloning site; ribosome binding site (SD) native to B. subtilis tagD; and gene bgaB, encoding a thermostable beta -galactosidase from B. stearothermophilis.

Applied and Environmental Microbiology, November 2001, p. 5349-5349, Vol. 67, No. 11
0099-2240/01   DOI:
Copyright © 2001, American Society for Microbiology. All rights reserved.



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