INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously present in the environment and have harmful effects on humans (3, 7). Recently, the potentials of P450 enzymes to transform PAHs have been evaluated to develop novel strategies in environmental bioremediation (6, 8, 21). Hydroxylation of PAHs by P450 enables the biodegradation of PAHs. Hydroxylated PAHs are more water-soluble and are the substrates for oxidases, such as peroxidase and laccase, that decompose phenolic compounds. The oxidation of PAHs by mammalian P450 enzymes has been extensively investigated (21, 22), but because of the low stability and activity of these P450s they are less promising from the perspective of the biodegradation of PAHs in the environment. Bacterial P450s are relatively stable and highly active and can often be prepared in large amounts using recombinant expression systems. Protein engineering of bacterial P450s with activities toward PAHs could be an alternative method for this purpose (15, 16, 18).

P450cam has been engineered for the degradation of PAHs with the highest turnover, 3.0 and 4.5/min, for phenanthrene and fluoranthene, respectively (6). Cytochrome P450 BM-3 from Bacillus megaterium is another one of the best-studied bacterial P450s whose crystal structure is available (1, 4, 9, 10, 17, 20). P450 BM-3 can be largely prepared by recombinant expression in Escherichia coli (11-13) and is a stable, highly active, and self-sufficient P450 consisting of a catalytic heme domain and a diflavin reductase domain in a single polypeptide of 119 kDa (17, 20). These properties are closer to application requirements than those of other P450s. However, this P450 has a well-defined substrate specificity and catalyzes the hydroxylation of long-chain fatty acids and the epoxidation of the double bonds of long-chain unsaturated fatty acids (1, 4, 10, 17, 20).

Protein engineering of P450 BM-3 to obtain activities towards substrates other than long-chain fatty acids, such as short-chain fatty acids or indole, was reported recently (11, 19). Just before we submitted this manuscript, Carmichael and Wong (2) reported the oxidation of pyrene, phenanthrene, and fluoranthene by mutants of P450 BM-3. However, we obtained another mutant showing higher activities for the oxidation of other PAHs such as naphthalene, fluorene, acenaphthene, acenaphthylene, and 9-methylanthracene. The triplet mutant (A74G/F87V/L188Q) obtained by us does not share any mutation with the mutants reported by Carmichael and Wong except that one of the mutated positions (Phe87) is shared with some of their mutants (2). A74 and L188 in our mutation essentially constitute the entrance of the substrate access channel and have hydrophobic interactions with substrates (9), while R47 and Y51 in the mutation of Carmichael and Wong act as anchors for the carboxylate group of the substrate (2). Here, we report our results.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Chemicals. 1,2-Dihydroxyacenaphthene was prepared by reduction of acenaphthenequinone with sodium hydrosulfite. Other chemicals were of analytical grade and are commercially available.

Randomization of specific codons of P450 BM-3. Three sites (Phe87, Leu188, and Ala74) were randomized by site-directed mutagenesis using a Stratagene QuikChange kit (La Jolla, Calif.) as described previously (11, 13). The following PCR primers were employed for the respective sites: for Phe87, 5'-GCAGGAGACGGGTTGNNNACAAGCTGGACG-3' and 5'-CGTCCAGCTTGTNNNCAACCCGTCTCCTGC-3'; for Leu188, 5'-GAAGCAATGAACAAGNNNCAGCGAGCAAATCCAG-3' and 5'-CTGGATTTGCTCGCTGNNNCTTGTTCATTGCTTC-3'; and for Ala74, 5'-GCTTTGATAAAAACTTAAGTCAANNNCTTAAATTTGTACG-3' and 5'-CGTACAAATTTAAGNNNTTGACTTAAGTTTTTATCAAAGC-3'.

Expression and purification of WT and mutant P450 BM-3. The wild-type (WT) and mutant P450 BM-3 genes were expressed under the control of the strong, temperature-inducible P_RP_L-promoter of pCYTEXP1 in E. coli strain UM2, a catalase-deficient strain (14), as described previously (12). Cell disruption, purification, and determination of the concentration of the purified enzyme were carried out as described elsewhere (13).

Isolation of mutants. Some mutants of P450 BM-3 produced pigments in the culture broth after induction of the expression of their genes in E. coli UM2. Structural analysis of the major pigment indicated that it might have a polycyclic aromatic structure. Thus, we used the pigments as indicators to screen mutants with higher activities towards PAHs. From the mutant created by randomized mutagenesis of the codon of the respective site, 100 colonies were isolated to ensure that most of the 19 possible kinds of amino acids were tested. Under these conditions, the probability of obtaining each amino acid is greater than 95%, except for tryptophan and methionine, for which the probability is 79% (12). The selected colonies were cultured in tubes containing 1 ml of Luria-Bertani medium at 30°C for 3 h, and then the temperature was shifted to 42°C for 10 h to induce the expression of P450 BM-3 mutant genes and to allow the production of pigments. After cells had been separated by centrifugation, the absorbance of the supernatants containing pigments was measured at 545 nm. The mutant that produced the largest amount of pigments among all the mutants at the same site was used as a template for the next site-specific randomized mutagenesis. All mutations described in this report have been confirmed by DNA sequencing.

Enzyme activity assay. To measure the NADPH consumption rate, 60 µl of a 2.5 µM P450 sample was mixed with 480 µl of 0.1 M Tris-HCl buffer (pH 7.4) containing 6 µl of substrate dimethyl sulfoxide (DMSO) stock solution. The concentrations of the substrate stock solutions were 200, 20, 30, 50, and 15 mM for naphthalene, fluorene, acenaphthene, acenaphthylene, and 9-methylanthracene, respectively. After 2 min of standing, 60 µl of 3.5 mM NADPH was added to start the reaction. NADPH consumption was monitored at 340 nm with a Shimadzu MultiSpec-1500 spectrophotometer (Kyoto, Japan) at 25°C for 20 s (or 150 s for WT, because of its low activity). The NADPH concentration was calculated using _M = 6,200 M¹ cm¹ (23).

GC and GC-MS analysis. GC analysis was performed with a Shimadzu GC-17A gas chromatograph equipped with a flame ionization detector and a split injection system and fitted with a capillary column (DB-17, 30 m by 0.554 mm internal diameter; J & W Scientific, Folsom, Calif.). The column temperature was initially 100°C, and then it was raised to 280°C at the rate of 5°C/min and maintained at that temperature for 20 min. The injector and detector were operated at 280°C. Helium was used as the carrier gas at 30 kPa/cm². GC-MS analysis was performed on a GC-MS QP5050 (Shimadzu) with a GC-17A gas chromatograph equipped with a capillary column (HR-1; 25 m by 0.25 mm internal diameter; Shinwa Kako, Kyoto, Japan) at 150°C. Helium was used as the carrier gas at 225 kPa/cm². MS was performed in the electron impact mode at 70 eV with a source temperature of 250°C. Split injection was employed with the injection port at 250°C.

HPLC analysis. HPLC analysis was performed to determine the amounts of 1-naphthol and 2-naphthol. The naphthalene reaction samples were extracted twice with chloroform, 6 ml in total, evaporated to dryness, and then dissolved in 5% DMSO for HPLC analysis. HPLC analysis was carried out on a C₁₈ reverse-phase column (Cosmosil 5C AR-II; 4.6 mm internal diameter by 250 mm; Nacalai Tesque, Kyoto, Japan) and eluted at 1.0 ml/min with a methanol-water gradient containing 1% acetic acid. The methanol gradient was as follows: 0 to 4 min, 0% (vol/vol); 4 to 10 min, 0 to 28%; 10 to 50 min, 28 to 80%; 50 to 60 min, 80%. The absorbance of the eluent was monitored at 275 nm.

Determination of binding spectra. The substrate-induced spectral shifts of P450 BM-3 enzymes were recorded with a Shimadzu MultiSpec-1500 spectrophotometer at 30°C. Naphthalene, fluorene, acenaphthene, and acenaphthylene dissolved in DMSO at concentrations of 300, 3, 15, and 15 mM, respectively, were freshly prepared for analysis. For each substrate, the substrate solution was added in small amounts to the enzyme solution containing 3.8 µM P450 in 0.1 M Tris-HCl (pH 7.4) in a cuvette, and the same amount of DMSO was added to the reference cuvette containing the enzyme solution. For each substrate for any enzyme, triplicate binding assays with 18 substrate concentrations were performed. The change in absorbance (A) was determined by subtracting A₄₂₅ (trough) from the A₃₉₀ (peak). The maximum heme spin-state shift of WT binding to palmitic acid was taken as a 100% heme spin-state shift. Data analysis and calculations were carried out according to methods described elsewhere (23).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Isolation of P450 BM-3 mutants for PAH oxidations. Mutants of P450 BM-3 for PAH oxidations were obtained by increasing the productivity of pigments with polycyclic aromatic structure in the culture broth through three steps of site-specific randomized mutagenesis. Phe87 was selected as the first site for mutation because several mutants which have a substitution at this site with Val, Ala, or Gly were initially found to produce the pigments. Leu188 and Ala74 were selected as the second and third sites for mutation according to the results of previous work on the hydroxylation of indole and shorter-chain fatty acids (11, 12). After the first mutation, 14 colonies produced pigments after induction and the 1 producing the highest amount of pigments was selected for DNA sequencing, which revealed the substitution of Phe87 by Val (F87V). The F87V mutant was used as the template DNA for the second round of site-specific randomized mutagenesis at Leu188. Seventy-two colonies produced pigments during the cultivation and the one exhibiting the highest activity was found to contain a new substitution of Leu188 by Gln besides Phe87Val (F87V/L188Q). The A₅₄₅ due to the pigment production of the culture of this double mutant was 2.3 times higher than that of F87V. This double mutant DNA was used as the template for the third round of site-specific randomized mutagenesis at Ala74. Seventy-seven colonies produced pigments and the one producing the highest amount of pigments was found to be a triple mutant containing a new substitution of Ala74 by Gly besides the Phe87Val and Leu188Gln mutations (A74G/F87V/L188Q). The A₅₄₅ of the culture of this triplet mutant was 1.2 times higher than that of F87V/L188Q.

PAH oxidation activity. The WT showed quite low NADPH consumption rates and coupling efficiencies for all of the PAHs (Tables 1 and 2). The first substitution of Phe87Val improved the NADPH consumption rates from several- to nearly 50-fold (Table 1) and the coupling efficiencies towards these PAHs several- to 200-fold (Table 2). In total, the first substitution improved the enzyme activities towards these PAHs by 2 to 3 orders of magnitude (Table 3). The second substitution of Leu188Gln also significantly improved the enzyme activities towards all the three-ring PAHs by several- to more than 30-fold. The third substitution of Ala74Gly again greatly enhanced the enzyme activities towards all the PAHs, mainly by increasing the NADPH consumption rates. At last, the activities of the triplet mutant towards all these PAHs were 2 to 4 orders of magnitude higher than those of the WT.

Analysis of products from PAHs. The products derived from PAHs by the WT and all mutants of P450 BM-3 are summarized in Fig. 1. The WT and all mutants catalyzed the oxidation of naphthalene to a mixture of 96% 1-naphthol (retention time [RT], 36.2 min) and 4% 2-naphthol (RT, 34.8 min), as confirmed by coelution with authentic samples on HPLC. In our experiments, to correctly evaluate the reaction selectivity, the further oxidation of 1-naphthol or 2-naphthol was limited to a negligible level by using NADPH at a low concentration (0.4 mM).

View larger version (27K): [in this window] [in a new window]   FIG. 1.   Proposed reactions catalyzed by the P450 BM-3 mutants.

Analysis of substrate binding. Only the mutations described here did not cause a heme spin-state shift, i.e., the hemes in these mutant enzymes without substrate binding are all in a low spin-state. Substrate binding to P450 usually triggers a heme spin-state shift from low to high, and it can be monitored as the changes of P450 absorbance spectra. The heme spin-state shift and dissociation constant assay results are given in Table 4. The binding of 9-methylanthracene was not examined because of its strong absorbance in the spectral range of analysis. The first substitution of Phe87Val significantly improved the heme spin-state shift for all four PAHs but had nearly no effect on the dissociation constant for all four substrates. The further two substitutions had no significant effect on either the heme spin-state shift or the dissociation constant for all four substrates.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Phe87 plays a critical role in the control of PAH binding to P450 BM-3. The aromatic ring of Phe87 extends into the heme pocket and is positioned above the porphyrin plane (9), which may prevent PAH binding close to the active site. Replacement of Phe87 with Val does not significantly affect the affinity of P450 BM-3 for these PAHs, but it largely increases the PAH-induced heme spin-state shift (Table 4) and coupling efficiencies of NADPH utilization (Table 2). These results suggest that the PAHs bind close to the heme with better positioning in the active site with respect to the activated oxygen intermediate that is generated during the catalytic cycle.

The PAH oxidation rates of the triplet mutant were 3 to 4 orders of magnitude faster than those of the WT. Compared with the activities of most mammalian P450s with turnover numbers of around 1.0/min (5), the activities of the triplet mutant towards these substrates are quite high. Coupling efficiency is an important factor for determining the substrate oxidation rate. The highest couplings obtained on engineering of P450cam towards phenanthrene and fluoranthene were only 1.3 and 3.1%, respectively (6). The coupling efficiencies of the triplet mutant towards these three-ring PAHs were improved 1 to 2 orders of magnitude from that of the WT, and the values themselves are also significantly high (Table 2).

The activities of P450 BM-3 towards pyrene, phenanthrene, and fluoranthene were also increased by the combination of the three mutations (R47L/Y51F/A264G) (2). However, there is no common mutation between this mutant and our triplet mutant (F87V/L188G/A74G). Therefore, it should be possible, perhaps with the help of X-ray structure analysis data, to further increase the activity towards PAHs by combining additional mutation(s). Such work and further study on application of the P450 BM-3 mutant for the biodegradation of PAHs by whole cells coexpressing an NADPH regeneration enzyme are in progress.