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Applied and Environmental Microbiology, February 2003, p. 740-746, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.740-746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of a Saccharomyces cerevisiae Mutant with Improved Ability To Utilize Xylose Shows Enhanced Expression of Proteins Involved in Transport, Initial Xylose Metabolism, and the Pentose Phosphate Pathway

C. Fredrik Wahlbom,¹ Ricardo R. Cordero Otero,² Willem H. van Zyl,² Bärbel Hahn-Hägerdal,^1* and Leif J. Jönsson^1,

Department of Applied Microbiology, Lund University, Lund, Sweden,¹ Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa²

Received 15 April 2002/ Accepted 10 September 2002

Top Abstract Introduction Materials and Methods Results Discussion References

 
Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samples for analysis were withdrawn from chemostat cultures. The characteristics of S. cerevisiae TMB 3399 and TMB 3400 grown on glucose and on a mixture of glucose and xylose, as well as of S. cerevisiae TMB 3400 grown on only xylose, were investigated. The strains were cultivated under chemostat conditions at a dilution rate of 0.1 h^-1, with feeds consisting of a defined mineral medium supplemented with 10 g of glucose liter^-1, 10 g of glucose plus 10 g of xylose liter^-1 or, for S. cerevisiae TMB 3400, 20 g of xylose liter^-1. S. cerevisiae TMB 3400 consumed 31% more xylose of a feed containing both glucose and xylose than S. cerevisiae TMB 3399. The biomass yields for S. cerevisiae TMB 3400 were 0.46 g of biomass g of consumed carbohydrate^-1 on glucose and 0.43 g of biomass g of consumed carbohydrate^-1 on xylose. A K_s value of 33 mM for xylose was obtained for S. cerevisiae TMB 3400. In general, the percentage error was <20% between duplicate microarray experiments originating from independent fermentation experiments. Microarray analysis showed higher expression in S. cerevisiae TMB 3400 than in S. cerevisiae TMB 3399 for (i) HXT5, encoding a hexose transporter; (ii) XKS1, encoding xylulokinase, an enzyme involved in one of the initial steps of xylose utilization; and (iii) SOL3, GND1, TAL1, and TKL1, encoding enzymes in the pentose phosphate pathway. In addition, the transcriptional regulators encoded by YCR020C, YBR083W, and YPR199C were expressed differently in the two strains. Xylose utilization was, however, not affected in strains in which YCR020C was overexpressed or deleted. The higher expression of XKS1 in S. cerevisiae TMB 3400 than in TMB 3399 correlated with higher specific xylulokinase activity in the cell extracts. The specific activity of xylose reductase and xylitol dehydrogenase was also higher for S. cerevisiae TMB 3400 than for TMB 3399, both on glucose and on the mixture of glucose and xylose.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Xylose is one of the most abundant building blocks in hemicellulose (22) and thus represents a large fraction of all organic carbon. Saccharomyces cerevisiae, the preferred organism for industrial ethanol production, cannot utilize xylose. However, this trait is necessary to make the production of fuel ethanol from xylose-rich lignocellulose cost-effective (28).

Recently, a diploid strain of S. cerevisiae (26) was transformed with the genes coding for xylose reductase (XR) and xylitol dehydrogenase (XDH) from the xylose-utilizing yeast Pichia stipitis, as well as with the S. cerevisiae gene encoding xylulokinase (XK) (29a). The resulting transformant, S. cerevisiae TMB 3399, had a maximum specific growth rate of 0.025 h^-1 when cultivated in a defined mineral medium supplemented with xylose. Chemical mutagenesis using ethyl methanesulfonate generated mutants with improved growth rate and xylose utilization. The best mutant selected was designated S. cerevisiae TMB 3400 and showed a >5-fold increase in growth rate, 0.14 h^-1, when cultivated under the same conditions as S. cerevisiae TMB 3399. The mutant, S. cerevisiae TMB 3400, also showed lower xylitol production than S. cerevisiae TMB 3399 when the strains were cultivated on xylose under oxygen-limited and anaerobic conditions.

Microarray technology permits the quantification of genome-wide mRNA expression (31). The technique offers the possibility to characterize differences in transcription level as a function of strain difference or cultivation condition. In S. cerevisiae, microarray technology has, among other things, been used to compare the mRNA expression during growth under aerobic and anaerobic conditions (25) and during a shift from growth on a fermentable carbon source to growth on a nonfermentable carbon source (10).

To identify genes beneficial for growth on xylose, microarray technology was used to compare mRNA expression in S. cerevisiae TMB 3399 and TMB 3400, as well as the growth of these two strains on glucose and xylose. S. cerevisiae TMB 3399 was cultivated in batch and chemostat cultures at a dilution rate of 0.1 h^-1 in a defined mineral medium supplemented with either glucose or a mixture of glucose and xylose. S. cerevisiae TMB 3400 was cultivated under the same conditions, as well as by using a feed containing only xylose. At steady state, samples were withdrawn for microarray analysis; analysis of XR, XDH, and XK activities; and analysis of substrate consumption and product formation.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Strains, medium, and cultivation conditions.
The genes encoding XR and XDH from P. stipitis, as well as XK from S. cerevisiae, were integrated into the genome of S. cerevisiae TMB 3399 by using the plasmid YIpXR/XDH/XK (29a). YIpXR/XDH/XK carries the genes encoding XR, XDH, and XK, as well as the ß-lactamase gene and a HIS3 marker gene (6). In this plasmid, the gene encoding XR is under the control of the ADH1 promoter, whereas the genes encoding XDH and XK are under the control of the PGK1 promoter.

S. cerevisiae TMB 3399 and S. cerevisiae TMB 3400 were stored at -80°C and streaked on yeast extract-peptone-dextrose plates 1 day prior to the inoculation of the precultures. A defined mineral medium, including vitamins and trace elements, was used in all fermentation experiments (27). The carbon and energy source consisted of either 10 g of glucose, 10 g of glucose plus 10 g of xylose, or 20 g of xylose liter^-1. Antifoam (Dow Corning Antifoam RD Emulsion; BDH Laboratory Supplies, Poole, England) was added at a concentration of 0.5 ml per liter.

The precultures consisted of 100 ml of medium containing 10 g of glucose liter^-1 in 1,000-ml baffled shake flasks. They were incubated at 30°C and 140 rpm in an orbital incubator (INR-200; Gallenkamp, Leicester, United Kingdom). The precultures used for the cultivation of S. cerevisiae TMB 3400 on pure xylose were cultivated under the same conditions but with 20 g of xylose liter^-1 as the carbon source. The bioreactors were inoculated with 20 mg of cells harvested in the late exponential phase liter^-1.

Batch and chemostat fermentation was conducted at 30°C in 2-liter Biostat A bioreactors (B. Braun Biotech International, Melsungen, Germany). The working volume of the bioreactors was 1,200 ml, and 3 M NaOH was used to automatically maintain the pH at 5.5. The airflow rate of 1 liter min^-1 was controlled by mass flow meters (HI-TECH; Bronkhorst, Ruurlo, The Netherlands), and the dissolved oxygen tension was at least 30% of the maximum. Duplicate fermentation experiments were performed for each feed composition.

Sampling, preparation of cRNA, microarray analysis, and analysis of substrates and products.
Steady-state conditions were assumed when the carbon dioxide evolution, oxygen consumption, and OD₆₂₀ had remained constant for three consecutive volume changes. This occurred at least six volume changes after feed initialization or feed change. Samples were then withdrawn for microarray analysis (2 x 50 ml), enzymatic assays (50 ml), and for the analysis of dry weight, substrate consumption and product formation (50 ml). The biomass concentrations were between 5 and 8 mg ml^-1 (Table 1).

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TABLE 1. Feed concentrations, residual substrate concentrations, and specific productivities from chemostat cultivation of S. cerevisiae strains TMB 3399 and TMB 3400 as determined with glucose and xylose as the carbon sources

Cells for microarray analysis were kept on ice for 30 min. The cell samples were subsequently centrifuged for 5 min at 5,000 x g and 4°C. The cells were then washed twice with ice-cold AE buffer (50 mM sodium acetate, pH 5.2; 10 mM EDTA). The cell pellets were frozen in liquid nitrogen and stored at -80°C while awaiting further processing. Total RNA was isolated by using the hot-phenol method (21) and mRNA was purified by using the Oligotex mRNA Mini Kit (Qiagen, Hilden, Germany). The synthesis of cDNA was performed by using the Superscript choice system (Gibco-BRL/Life Technologies, Gaithersburg, Md.) and a T7-(dT)24 oligonucleotide primer (Genset, Paris, France). In vitro transcription was carried out by using the Enzo BioArray High-Yield RNA transcript labeling kit (Enzo Diagnostics, Farmingdale, N.Y.). The cRNA was fragmented, and the hybridization mixture was prepared according to the recommendations of the manufacturer of the microarrays (Affymetrix, Santa Clara, Calif.). Hybridization, washing, staining, and scanning of the microarrays (GeneChip yeast genome S98 arrays) were performed by using a Hybridization Oven 320, a Fluidics Station 400, and a GeneArray scanner (all from Affymetrix). Data were collected and processed by using the Microarray Suite software, version 4.0 (Affymetrix).

Cells for enzymatic activity measurements were washed once with distilled water and treated with yeast protein extraction solution (Y-PER; Pierce, Rockford, Ill.) for 20 min at room temperature. The mixture was centrifuged for 5 min at 4°C and 5,000 rpm, and the supernatant was used for enzymatic assays. XR, XDH, and XK were assayed as previously described (6), but triethanolamine buffer at pH 7.0 was used for the XDH assay instead of glycine buffer at pH 9.0, since the high pH of the glycine buffer caused precipitation of components in the extraction solution.

Glucose, xylose, xylitol, succinate, glycerol, acetate, and ethanol were analyzed by high-pressure liquid chromatography, as has been described previously (29). The composition of the outgoing gas was monitored continuously with a carbon dioxide and oxygen monitor type 1308 (Brüel & Kjaer, Copenhagen, Denmark) (4) by using photoacoustic and magnetoacoustic detection for CO₂ and O₂, respectively. The cell dry weight was determined by filtering a known volume of the culture broth through a 0.45-µm (pore-size) Supor membrane (Gelman Sciences, Ann Arbor, Mich.). The filter was weighed after being washed with 3 volumes of double-distilled water and dried in a microwave oven for 15 min at 350 W. The cell dry weight was determined in triplicate.

Construction of strains with open reading frame YCR020c overexpressed and deleted.
E. coli DH5 (Life Technologies, Rockville, Md.) was used for the cloning procedures. The p426ADH (ATCC 87377) multicopy vector (14) was used to overexpress the open reading frame YCR020c. This vector contains the ß-lactamase gene, the URA3 selection marker, and an ADH1 promoter and CYC1 terminator. The integrative plasmid YIpXR/XDH/XK (6) was used to introduce the genes encoding XR, XDH, and XK. The strains S. cerevisiae Y00000 (BY4741; MATa his3-1 leu20 met150) and S. cerevisiae Y03500 (BY4741; MATa his3-1 leu20 met150 YCR020c::kanMX4) were obtained from Euroscarf (Frankfurt, Germany).

Construction of xylose-utilizing strains with overexpression and deletion of YCR020c.
All enzymes used for cloning and restriction analysis were obtained from Fermentas (Vilnius, Lithuania) unless stated otherwise. Standard techniques were used for cloning, transformation, and analysis (1). Chromosomal DNA from S. cerevisiae TMB 3400 was prepared by using the Easy-DNA kit (Invitrogen, Carlsbad, Calif.) and was used as the template for PCR amplification of the open reading frame YCR020C. The PCR product was obtained by using the upstream primer 5'-GCA CTA GTA TGA GCT GTA CCA CTG ATA AGT TA-3' in combination with the downstream primer 5'-GCG AAT TCT TAG GCG TTG TAA CAA GAT TCA AAA-3'. The start codon is indicated in boldface, and the restriction sites for BcuI (SpeI) (upstream primer) and EcoRI (downstream primer) are underlined. The concentrations of nucleotides, Pwo DNA polymerase, primers, and Mg²⁺ ions were as recommended by the supplier (Roche, Mannheim, Germany). The PCR was performed in a GeneAmp PCR system 9700 (Perkin-Elmer Corp., Norwalk, Conn.), and the conditions employed were as follows: 94°C for 1 min, 55°C for 30 s, and 72°C for 1 min (10 cycles); 94°C for 1 min, 61°C for 30 s, and 72°C for 1.5 min (15 cycles); and 94°C for 1 min, 61°C for 30 s, and 72°C for 2.5 min (10 cycles). The PCR product was purified with the QiaQuick kit (Qiagen, Hilden, Germany) and, together with the vector p426 ADH, it was cleaved with BcuI and EcoRI and ligated with T4 DNA ligase to form the plasmid pFW5.

S. cerevisiae TMB 3151 was created by integrating the YIpXR/XDH/XK plasmid (6), linearized by digestion with PstI, into the HIS3 locus of the laboratory strain S. cerevisiae CEN.PK 113-11C (MATa his3-1 ura3-52). S. cerevisiae TMB 3151 was transformed with the plasmids p426ADH and pFW5, resulting in S. cerevisiae strains TMB 3152 and TMB 3155, respectively.

S. cerevisiae strains Y00000 and Y03500 were transformed with the YIpXR/XDH/XK plasmid in the same way as S. cerevisiae CEN.PK 113-11C and were named S. cerevisiae strains TMB 3159 and TMB 3160, respectively.

The recombinant strains, S. cerevisiae TMB 3152, TMB 3155, TMB 3159, and TMB 3160, were cultivated by using the same medium as described above. One-liter shake flasks containing 100 ml of medium supplemented with 20 g of glucose or 10 g of ethanol liter^-1, as well as 250-ml shake flasks containing 25 ml of medium supplemented with 10 g of xylulose or 20 g of xylose liter^-1 were incubated in a rotary shaker at 30°C. The growth rate was determined by measuring the optical density at 620 nm. Duplicate cultivation experiments were performed for each substrate and strain.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Chemostat cultivation of S. cerevisiae strains TMB 3399 and TMB 3400.
S. cerevisiae TMB 3399 and TMB 3400 were first cultivated in a batch, with the carbon source consisting of 10 g of glucose liter^-1, and from which the maximum specific growth rates were determined to be 0.44 ± 0.006 h^-1 for both strains. At the late exponential phase, chemostat cultivation was initiated by feeding with a defined mineral medium, first supplemented with glucose (10 g liter^-1) and, six volume changes later, with a mixture of 10 g of glucose plus 10 g of xylose liter^-1. Since only S. cerevisiae TMB 3400 and not S. cerevisiae TMB 3399 shows a maximum specific growth rate exceeding 0.1 h^-1 on xylose, which is required to prevent washout, only S. cerevisiae TMB 3400 was cultivated in chemostat with xylose as the sole carbon source. S. cerevisiae TMB 3400 was first cultivated in a batch supplemented with 20 g of xylose liter^-1, after which a feed of 20 g of xylose liter^-1 was added.

The biomass yield was ca. 0.47 g of biomass g of consumed carbohydrate^-1 for both strains during growth on glucose and glucose plus xylose, whereas it decreased to 0.43 g biomass g of consumed carbohydrate^-1 for S. cerevisiae TMB 3400 during growth on xylose only (Table 1). The specific glucose consumption was 247 and 231 mg g of biomass^-1 h^-1 for S. cerevisiae TMB 3399 and S. cerevisiae TMB 3400, respectively, when cultivated on glucose only. When grown on a mixture of glucose and xylose, S. cerevisiae TMB 3399 gave higher residual glucose and xylose concentrations, 0.09 and 4.6 g liter^-1, respectively, than S. cerevisiae TMB 3400, which gave no detectable glucose and only 2.9 g of xylose liter^-1. The specific glucose consumption was higher for S. cerevisiae TMB 3399: 160 mg g of biomass^-1 h^-1 compared to 142 mg g of biomass^-1 h^-1 for S. cerevisiae TMB 3400. The reverse was true for the specific xylose consumption: 85 mg g of biomass^-1 h^-1 for S. cerevisiae TMB 3399 and 97 mg g of biomass^-1 h^-1 for S. cerevisiae TMB 3400. A total of 2.5 mg g of biomass^-1 h^-1 xylitol was formed during the cultivation of S. cerevisiae TMB 3399. During growth on xylose only, S. cerevisiae TMB 3400 consumed 12.3 g liter^-1 of 20.8 g liter^-1 in the feed and the specific xylose uptake, 254 mg g of biomass^-1 h^-1, more than doubled in comparison to cultivation on the mixture of glucose and xylose. A K_s value of 4.9 g liter^-1, or 33 mM, was obtained by employing Monod kinetics (16), using D = 0.1 h^-1, D_crit = 0.14 h^-1, and s = 12.3 g liter^-1. S. cerevisiae TMB 3400 also produced a small amount of xylitol, 1 mg g of biomass^-1 h^-1. The carbon balances closed within 94 to 103% for all fermentation experiments.

mRNA expression levels.
The mRNA expression levels of the more than 6,000 genes in the genome of S. cerevisiae were monitored simultaneously by using DNA microarrays. In the present investigation, we chose to analyze the mRNA expression levels of the genes encoding sugar transporters and the enzymes involved in xylose metabolism, the pentose phosphate pathway, glycolysis, gluconeogenesis, and galactose metabolism (Table 2), as well as the genes involved in regulation (Table 3). All microarray experiments were performed in duplicate with cells from two independent fermentation experiments.

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TABLE 2. mRNA expression levels in S. cerevisiae strains TMB 3399 and TMB 3400 for genes coding for transporters, enzymes involved in xylose metabolism, the pentose phosphate pathway, glycolysis, glyoxylate shunt, gluconeogenesis, and galactose metabolism

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TABLE 3. mRNA levels of genes involved in regulation

There are 20 hexose transporters in S. cerevisiae (2), of which the mRNA expression levels for HXT4, HXT5, and HXT7, together with galactose permease, GAL2, changed during the different cultivation conditions and between the strains (Table 2). HXT5 was more highly expressed in S. cerevisiae TMB 3400 than in S. cerevisiae TMB 3399. The expression of GAL2 was about 100 times higher when xylose was the only sugar in the feed than when glucose was also present.

With regard to xylose metabolism, the heterologous genes encoding XR and XDH were not included in the analysis, since they were cloned from P. stipitis and therefore are not represented on the S. cerevisiae DNA microarrays. The expression of S. cerevisiae genes encoding enzymes with XR (YHR104w) (9) and XDH (YLR070c) (19) activities was analyzed. These two genes showed slightly increased expression during growth on only xylose. The XKS1 gene encoding XK was expressed at levels about twice as high in S. cerevisiae TMB 3400 as those in TMB 3399.

SOL3 and GND1, as well as TAL1 and TKL1, encoding enzymes in the oxidative and nonoxidative pentose phosphate pathway, respectively, were expressed at higher levels in S. cerevisiae TMB 3400 than in TMB 3399. Among the genes encoding glycolytic enzymes, HXK1, HXK2, ENO2 and, possibly, YDR516c showed lower expression levels when TMB 3400 was grown on only xylose. Genes encoding the gluconeogenetic enzyme phosphoenolpyruvate carboxykinase and the glyoxalate enzyme isocitrate lyase, as well as genes involved in galactose metabolism, were upregulated in S. cerevisiae TMB 3400 during growth on xylose only.

Among the genes involved in regulation, notably YBR083W and YPR199C were expressed at lower levels in S. cerevisiae TMB 3400 than in TMB 3399 (Table 3). The PET18 gene (YCR020C), which encodes a transcription regulator, was expressed at high levels in S. cerevisiae TMB 3400 during growth on the mixture of glucose and xylose. When S. cerevisiae TMB 3400 was grown on xylose only, the expression of MIG1 and MIG2 was lower, whereas the expression of CAT8 was higher.

Enzymatic activities.
The specific activities of enzymes involved in the initial xylose metabolism, i.e., XR, XDH, and XK, are given in Table 4. All three enzymes displayed higher activities in S. cerevisiae TMB 3400 than in S. cerevisiae TMB 3399 regardless of whether the feed contained only glucose or glucose plus xylose. The specific activity determined in the cell extracts from xylose-grown S. cerevisiae TMB 3400 was very low for all three enzymes.

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TABLE 4. Enzymatic activities of XR, XDH, and XK from chemostat cultivation of S. cerevisiae strains TMB 3399 and TMB 3400 on various feed concentrations of glucose and xylose

Characterization of PET18.
Among the genes encoding proteins involved in gene regulation that were differently expressed in S. cerevisiae TMB 3399 and its mutant S. cerevisiae TMB 3400 (Table 3), the effect of the transcription regulator encoded by PET18 on xylose utilization was further investigated. Strains with overexpression and deletion of PET18, as well as control strains, were developed and cultivated in shake flasks with a defined mineral medium supplemented with glucose, xylose, xylulose, and ethanol (Table 5). The effect of PET18 on glucose and ethanol growth was negligible. The growth on xylulose was not affected significantly when PET18 was overexpressed, but deletion of the gene slightly improved the growth rate. Xylose growth was hampered by overexpression, but the deletion did not change the performance of the strain.

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TABLE 5. Maximum specific growth rates of S. cerevisiae strains TMB 3152, TMB 3155, TMB 3159, and TMB 3160 grown in shake flasks in a defined mineral medium supplemented with glucose, ethanol, xylulose, and xylose

Top Abstract Introduction Materials and Methods Results Discussion References

 
S. cerevisiae TMB 3399, transformed with the genes encoding XR, XDH, and XK, showed a growth rate of 0.025 h^-1 (29a) and was subjected to chemical mutagenesis with ethyl methanesulfonate to further improve its ability to utilize xylose. The best-performing mutant, S. cerevisiae TMB 3400, showed a >5-fold increase in maximum specific growth on xylose (0.14 h^-1) (29a). Random mutagenesis combined with a good selection protocol generates strains that display the characteristics selected for, although the site(s) of the mutation(s) remains unknown. In the present investigation we report, for the first time, the use of microarray technology to characterize a recombinant strain of S. cerevisiae that is chemically mutated for better growth on a nonnatural substrate. All microarray experiments were performed in duplicate, starting with independent fermentation experiments, and the percentage error (difference from the mean as a fraction of the mean) generally was less than 20%. Thus, reproducible DNA microarray results were obtained for a diploid, industrial strain of S. cerevisiae.

In S. cerevisiae TMB 3400, the genes HXT5 (encoding a hexose transporter), XKS1 (encoding XK), and SOL3 and GND1 (coding for proteins in the oxidative pentose phosphate pathway), as well as, but to a lesser extent, TKL1 and TAL1 in the nonoxidative pentose phosphate pathway, were expressed at higher levels than in the parental strain S. cerevisiae TMB 3399. The higher expression of these genes could explain the improved growth rate of S. cerevisiae TMB 3400 on xylose, as well as its higher xylose uptake from a feed containing a mixture of xylose and glucose.

The HXT5 gene encodes a hexose transporter with moderate affinity for glucose (40 mM) (5). A previous investigation (5) reported abundant HXT5 expression during slow growth on glucose and during growth on nonfermentable carbon sources but, in contrast to our results, this was suggested to be glucose repressed.

When TMB 3400 and TMB 3399 were compared under the same conditions, either with glucose or with the glucose-xylose mixture, the higher XKS1 mRNA expression levels monitored for TMB 3400 (Table 2) agreed well with the higher XK activities measured in the cell extracts (Table 4). That the XK activity in S. cerevisiae TMB 3400 was higher than in TMB 3399 is also in agreement with a previous investigation of an S. cerevisiae strain mutated for improved xylose growth. The enzymatic activity of XK in the mutated S. cerevisiae IM2, with a maximum specific growth rate of 0.08 h^-1, was 60% higher than in its parent S. cerevisiae H that has a maximum specific growth rate of 0.03 h^-1 (24). Furthermore, XK activities were 5 to 10 times higher in mutants of the natural xylose-utilizing yeasts Pachysolen tannophilus (11) and Candida utilis (12), which were selected for improved utilization of xylose and xylitol, respectively.

The higher expression of HXT5, XKS1, SOL3, and GND1, as well as of TKL1 and TAL1, in S. cerevisiae TMB 3400 could be due to an altered expression of one or several transcription regulators acting on these genes. The transcription factors coded for by YCR020C, YBR083W, and YPR199C were differently expressed in S. cerevisiae TMB 3400 and TMB 3399. The open reading frame YCR020C encodes the transcription factor Pet18p, which was expressed at higher levels in S. cerevisiae TMB 3400 than in S. cerevisiae TMB 3399 during growth on the mixture of glucose and xylose. The decision to investigate the influence of PET18 on xylose utilization was based on its reported effect on carbon utilization. Mutants defective in PET18 have been reported to be unable to grow on nonfermentable carbon sources (30). However, the strain in which PET18 was deleted was capable of growth on ethanol. Furthermore, xylose growth seemed to be unaffected by deleting and by overexpressing PET18. The transcription factors encoded by YBR083W and YPR199C deserve further attention and their effect on xylose utilization in S. cerevisiae will be reported in a forthcoming study.

The development of the superior xylose-utilizing S. cerevisiae TMB 3400 allowed us to perform a physiological comparison, as well as a comparison of mRNA expression levels, between growth levels on xylose and glucose. The first chemostat cultivation of a recombinant S. cerevisiae strain on xylose as the sole carbon source is presented here. S. cerevisiae TMB 3400 consumed ca. 40% of the xylose in the feed. This permitted us to determine the K_s value for xylose (33 mM), which is ca. 60 times higher than the corresponding value reported for growth on glucose (0.55 mM) (27). The biomass yield was lower on xylose than on glucose; therefore, S. cerevisiae TMB 3400 apparently cannot obtain as much energy from xylose as from glucose, which points toward a difference in the metabolism of these substrates. The gluconeogenetic gene encoding phosphoenolpyruvate carboxykinase and the glyoxylate gene encoding isocitrate lyase were upregulated during growth on xylose. When carbon is channeled through the glyoxylate shunt, ATP (GTP) is lost due to a reduced flux between succinyl coenzyme A and succinate. A lower ATP yield may be responsible for the lower biomass yield of S. cerevisiae TMB 3400 on xylose than on glucose. In a previous investigation, batch cultivation of S. cerevisiae TMB 3399 and 3400 showed biomass yields of 0.39 and 0.41 g of biomass g of xylose^-1, respectively (29a). The fact that both the transformant S. cerevisiae TMB 3399 and the mutant S. cerevisiae TMB 3400 displayed similar biomass yields on xylose indicates that the lower biomass yield on xylose is not due to a mutation. The genes encoding phosphoenolpyruvate carboxykinase and isocitrate lyase are upregulated by CAT8 (3, 17, 18), which in turn is repressed by the glucose transcription regulator Mig1p (8). Our results show higher mRNA levels of CAT8 and lower expression of MIG1 during growth on xylose in comparison to growth on glucose.

MIG1 also regulates genes involved in galactose metabolism. The analysis of S. cerevisiae TMB 3400 showed that galactose permease, galactokinase, galactose 1-phosphate uridyl transferase and UDP-glucose 4-epimerase were expressed at higher levels during growth on only xylose in comparison to growth on glucose or on a combination of glucose and xylose. In the presence of glucose, MIG1 causes repression of the transcription of genes involved in galactose metabolism (15). However, galactose has been reported to be necessary for the full initiation of transcription (23, 32), and it remains to be elucidated whether the increased expression of these genes in S. cerevisiae TMB 3400 is mediated by xylose or is due to a mutation.

Some of the genes encoding glycolytic enzymes, notably HXK2, showed lower expression levels when TMB 3400 was grown on only xylose. It has been demonstrated that the induction of glycolytic genes requires increased concentrations of metabolites in the early stages of glycolysis (7, 13, 20). These concentrations might be insufficient for the full induction of glycolysis during xylose growth.

 
Energimyndigheten (The Swedish National Energy Administration) provided financial support for this study.

We thank Carl A. K. Borrebaeck for providing the facilities for DNA microarray analysis.

 
* Corresponding author. Mailing address: Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden. Phone: 46-46-222-8428. Fax: 46-46-222-4203. E-mail: Barbel.Hahn-Hagerdal{at}tmb.lth.se.

Present address: Biochemistry, Division for Chemistry, Karlstad University, Karlstad, Sweden.

Top Abstract Introduction Materials and Methods Results Discussion References

 

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Applied and Environmental Microbiology, February 2003, p. 740-746, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.740-746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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