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Applied and Environmental Microbiology, June 2003, p. 3492-3499, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3492-3499.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay

Yang Hong,1 Mark E. Berrang,2 Tongrui Liu,1 Charles L. Hofacre,1 Susan Sanchez,3 Lihua Wang,4 and John J. Maurer1,5*

Department of Avian Medicine,1 Athens Diagnostic Laboratory, College of Veterinary Medicine,3 Department of Statistics, School of Arts and Sciences, The University of Georgia, Athens, Georgia 30602,4 Agricultural Research Service, U.S. Department of Agriculture, Russell Research Center, Athens, Georgia 30604,2 Center for Food Safety and Quality Enhancement, College of Agriculture and Environmental Sciences, The University of Georgia, Griffin, Georgia 302235

Received 30 October 2002/ Accepted 10 March 2003


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ABSTRACT
 
Contamination of retail poultry by Campylobacter spp. and Salmonella enterica is a significant source of human diarrheal disease. Isolation and identification of these microorganisms require a series of biochemical and serological tests. In this study, Campylobacter ceuE and Salmonella invA genes were used to design probes in PCR-enzyme-linked immunosorbent assay (ELISA), as an alternative to conventional bacteriological methodology, for the rapid detection of Campylobacter jejuni, Campylobacter coli, and S. enterica from poultry samples. With PCR-ELISA (40 cycles), the detection limits for Salmonella and Campylobacter were 2 x 102 and 4 x 101 CFU/ml, respectively. ELISA increased the sensitivity of the conventional PCR method by 100- to 1,000-fold. DNA was extracted from carcass rinses and tetrathionate enrichments and used in PCR-ELISA for the detection of Campylobacter and S. enterica, respectively. With PCR-ELISA, Salmonella was detected in 20 of 120 (17%) chicken carcass rinses examined, without the inclusion of an enrichment step. Significant correlation was observed between PCR-ELISA and cultural methods (kappa = 0.83; chi-square test, P < 0.001) with only one false negative (1.67%) and four false positives (6.67%) when PCR-ELISA was used to screen 60 tetrathionate enrichment cultures for Salmonella. With PCR-ELISA, we observed a positive correlation between the ELISA absorbance (optical density at 405 nm) and the campylobacter cell number in carcass rinse, as determined by standard culture methods. Overall, PCR-ELISA is a rapid and cost-effective approach for the detection and enumeration of Salmonella and Campylobacter bacteria on poultry.


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INTRODUCTION
 
An estimated 76 million cases of food-borne illnesses occur annually in the United States, of which 5,200 are fatal (37). Campylobacter and Salmonella bacteria account for 2.4 million and 1.4 million of these cases, respectively. Poultry and poultry products have been implicated as a major source of Campylobacter and Salmonella infection in humans (5, 15). Salmonella bacteria generally cause a self-limiting gastroenteritis in healthy adults and occasionally cause a sometimes-fatal bacteremia in the very young or the elderly (62). For Campylobacter species, most human infections are caused by C. jejuni and C. coli (3). Although rarely fatal, C. jejuni infection can sometimes cause a debilitating neurological disorder, Guillain-Barré syndrome (11). A recent study revealed that 70.7% of the poultry carcasses and 91% of the retail chicken products examined were contaminated with Campylobacter (63). Based on U.S. Department of Agriculture (USDA)-Food Safety and Inspection Service surveillance, the prevalence of Salmonella contamination of freshly processed poultry carcasses was reported to be 11.4% in 1999 and 9.1% in 2000 (http://www.usda.gov). Hazard analysis and critical control point systems for poultry are being implemented currently in the United States and other countries (39). Beginning in 1996, large poultry processors have been required to meet performance standards for reducing the frequency of Salmonella contamination (2). These events and the short shelf life of meat products have stimulated the development and implementation of rapid and specific detection methods for those pathogens in poultry.

Conventional cultural methods for detecting Salmonella and Campylobacter spp. involve enrichment in selective broth, followed by isolation on selective differential agar. Campylobacter spp. have demanding growth requirements because they need to be incubated under microaerobic conditions (5% O2, 10% CO2, and 85% N2), which makes the task of isolation laborious and costly (20). Both a primary and a secondary enrichment culture are necessary for isolating Salmonella enterica from foods. Isolation can therefore be labor-intensive and expensive when large numbers of samples must be processed (34). Hence, there is need for a sensitive noncultural detection method for these food-borne pathogens.

Molecular techniques such as PCR have proven to be specific and sensitive methods for detecting infectious pathogens (7, 29). PCR can detect as few as 100 bacteria per milliliter (18). Direct identification of organisms without prior isolation and purification from samples such as urine, sputum, poultry carcass rinses, and fecal material can be done (30, 50, 57, 58). The PCR-enzyme-linked immunosorbent assay (ELISA) has also been used to detect Campylobacter and S. enterica in environmental water, stools, and poultry samples and is more sensitive than conventional gel-based PCR. PCR-ELISA involves incorporation of chemically tagged nucleotides into the PCR amplicon and subsequent detection of the PCR product with antibody-enzyme conjugate that recognizes the unique chemical label present in the incorporated nucleotides (33). Under most circumstances, samples require an enrichment step or must be concentrated to improve the likelihood of detecting the target organisms by PCR (24, 35, 46, 48). In this study, we designed, validated, and implemented a specific, multiplex PCR primer set and probes based on the Salmonella virulence gene invA (12) and the Campylobacter ceuE gene, which encodes a lipoprotein involved in siderophore transport (42), for PCR-ELISA to screen poultry carcasses for these two important food-borne pathogens.


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MATERIALS AND METHODS
 
Isolation of Salmonella and Campylobacter from poultry and the poultry environment.
For the Campylobacter study, 32 chicken carcasses were removed from shackles in a commercial processing plant. Sixteen were collected prior to the inside-outside washer, and 16 were collected immediately following the inside-outside washer. Each carcass was individually placed into a sterile plastic bag, sealed, covered with ice, and transported to the laboratory. Sterile distilled water (100 ml) was used to rinse the carcass, and the rinse was cultured for Campylobacter (19). The carcass rinse was serially diluted (1:10) in phosphate-buffered saline (PBS), and Campylobacter bacteria were enumerated by plating the mixture in duplicate onto Campy-Cefex agar (54). One-tenth milliliter was spread onto each plate with a sterile plastic inoculating loop, and plates were subsequently incubated at 42°C for 36 to 48 h in a microaerobic environment (5% O2, 10% CO2, and 85% N2). Colonies characteristic of Campylobacter were counted. Each colony type counted as Campylobacter from each sample was confirmed as a member of the genus by microscopic examination for typical helical cellular morphology and detection of darting motility. Each colony was identified as C. jejuni or C. coli with a Campylobacter species-specific latex agglutination test kit (Integrated Diagnostics Inc., Baltimore, Md.) (8).

For detection of S. enterica, commercial broiler houses were sampled by using drag swabs, which were gauze pads soaked with double-strength skim milk (13). Each drag swab was then placed in 100 ml of tetrathionate brilliant green broth (TBG) (Becton Dickinson and Co., Sparks, Md.) and incubated at 41.5°C for 18 h (10). Chicken carcasses collected at the processing plant prior to and immediately after chilling were rinsed with 250 ml of buffered peptone water (59). Ten milliliters of the carcass rinse was used to inoculate 90 ml of TBG enrichment broth, and the enrichment broth was incubated at 41°C for 18 h (10). A loopful of the enrichment broth was streaked onto an XLT4-BGN biplate (Becton Dickinson and Co.) followed by overnight incubation at 37°C (25). H2S-producing colonies on XLT4 plates were identified as Salmonella by using poly(O) Salmonella-specific antiserum (Becton Dickinson and Co.) in a whole-cell agglutination assay.

PCR-ELISA for detecting C. jejuni, C. coli, and S. enterica.
DNA was extracted from TBG enrichment broth of the drag swabs as described by Liu et al. (34). The Mo Bio DNA purification and isolation kit (Mo Bio Laboratories Inc., Solana Beach, Calif.) was used to extract DNA from Campylobacter and Salmonella in chicken carcass rinses. Carcass rinse aliquots (50 ml) were held at 4°C for 30 min, and fat was then separated by centrifugation at 820 x g for 15 min. The supernatant containing bacteria was transferred to a second tube, and cells were sedimented by centrifugation at 19,000 x g for 15 min. The resulting bacterial pellet was resuspended in 1 ml of PBS (pH 7.0) and transferred to 2 ml of Bead Solution tubes. DNA was isolated and purified according to the protocol described by the manufacturer and was then used directly in PCR. The final volume of DNA eluted from the DNA-affinity column was 30 µl. PCR was done to evaluate the quality of the templates by using universal 16S rRNA primers and confirming the expected size amplicon (995 bp) on an agarose gel (43). DNA was separated on a 1.6% agarose-1x Tris-acetate-EDTA (pH 8.0) gel with ethidium bromide (0.2 µg/ml) at 100 V (49). A 100-bp ladder (Roche Molecular Biochemicals, Indianapolis, Ind.) was used as a molecular size standard for determining the size of PCR products.

Campylobacter ceuE, a gene encoding a 34.5- to 36.2-kDa lipoprotein component of a binding-protein-dependent transport system for the siderophore enterochelin, was analyzed with GeneRunner (Hastings Software, Hastings, N.Y.) DNA analysis software to search for PCR primers specific for C. jejuni (GenBank accession no. NC 002163) and C. coli (GenBank accession no. X88849) (42) (Table 1). Salmonella virulence gene invA was used to design PCR primers specific for S. enterica (GenBank accession no. M90846) (12) (Table 1). The capture probe was also designed to anneal to the central region of the PCR amplicon. A biotin molecule was added to the 3' end of the probe to prevent it from serving as a primer in the PCR, and this oligonucleotide was used to bind the PCR amplicon to the bottom of the ELISA plate coated with streptavidin. Primers and probes were synthesized at the Molecular Genetics Instrumentation Facility at the University of Georgia with the ABI Model 394 oligonucleotide synthesizer. PCR was done with a Rapidcycler hot-air thermocycler (Idaho Technologies, Idaho Falls, Idaho) with 10-µl-capacity capillary tubes (61). The 10-µl PCR mix consisted of 3.0 mM MgCl2, 50 mM Tris (pH 8.3), 0.25 mg of bovine serum albumin/ml, 1.0 µM (each) primer, 2.0 pM biotin-labeled Campylobacter or Salmonella probe, 0.2 mM digoxigenin (DIG)-labeled deoxynucleotides, 1.0 U of Taq DNA polymerase, and 1 µl of DNA template. The PCR program parameter consisted of a hold at 94°C for 1 min; then 94°C for 0 s, 55°C for 0 s, and 72°C for 20 s with a ramping rate of 2.0°C/s between the annealing and extension steps for 25, 30, or 40 cycles; and a final extension at 72°C for 4 min. An additional cycle was added to the final stage of the PCR amplification to anneal the detection probe to PCR amplicons. For Campylobacter, this additional cycle step consisted of 94°C for 1 min and 42°C for 16 min with a ramping rate of 2.0°C/s between the annealing and extension steps, whereas the PCR program for Salmonella had a final step of 94°C for 1 min and 50°C for 16 min with a ramping rate of 2.0°C/s between the annealing and extension steps. PCR amplicons were detected with a DIG detection ELISA kit (ABTS [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)]; Roche Molecular Biochemicals, Mannheim, Germany). PCR product was placed in streptavidin-coated wells containing conjugate buffer (PBS [pH 7.0] plus 0.1% blocking reagent) and incubated at 37°C for 1 h. The wells were then washed five times with wash buffer (PBS plus 0.1% Tween 20), with the buffer being left in wells for 2 min with each wash. Anti-DIG antibody-peroxidase conjugate (at the dilution specified by the manufacturer) was added to each well and incubated at 37°C for 1 h before the wells were washed five times with the wash buffer. Finally, the peroxidase substrate was added to wells and optical density (OD) values were recorded with an ELISA plate reader (Molecular Devices Corp., Sunnyvale, Calif.) ({lambda} = 405 nm) after 1 h of incubation at room temperature.


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  		TABLE 1. PCR primers and ELISA capture probes for S. enterica, C. coli, and C. jejuni

To determine the detection limit for Campylobacter, genomic DNA was extracted with the Puregene DNA isolation kit (Gentra Systems, Inc., Minneapolis, Minn.) from C. jejuni. DNA was quantified with the GeneQuant II RNA-DNA calculator (Amersham Biosciences Corp., Piscataway, N.J.). Tenfold serial dilutions were made from the extracted DNA (34.6 µg/µl) and tested with PCR-ELISA. Based on the amount of DNA in each bacterial cell (8.8 x 10-15 g), the detection limit was converted from the unit micrograms per microliter to CFU per milliliter (27). To determine the detection limit for S. enterica, we first cultured the bacteria overnight in Luria-Bertani medium and DNA was extracted according to the cetyltrimethylammonium bromide protocol of Ausubel et al. (4). Tenfold serial dilutions were made from extracted DNA. PCR-ELISA was then performed as described above. Specificity was determined for PCR-ELISA for S. enterica serotypes Typhimurium (n = 4), Enteritidis (n = 3), Hadar (n = 2), Heidelberg (n = 2), Kentucky (n = 2), Agona (n = 1), Anatum (n = 1), Bredney (n = 1), Chester (n = 1), Choleraesuis (n = 1), Infantis (n = 1), Indiana (n = 1), Give (n = 1), London (n = 1), Montevideo (n = 1), Mbandaka (n = 1), Muenchen (n = 1), Ohio (n = 1), Schwarzengrund (n = 1), and St. Paul (n = 1) and for other Enterobacteriaceae including Citrobacter freundii (n = 1), Klebsiella pneumoniae (n = 1), Serratia fonticola (n = 1), Enterobacter cloacae (n = 1), and Escherichia coli (n = 10). Specificity for Campylobacter was determined with C. jejuni (n = 12), C. coli (n = 6), C. lari (n = 2), C. upsaliensis (n = 1), and C. fetus ATCC 25936 (n = 1), and the other {varepsilon}-proteobacteria including Helicobacter hepaticus (n = 1), Arcobacter cryaerophilus ATCC 49942 (n = 1), and Arcobacter butzleri ATCC 49616 (n = 1). C. jejuni and C. coli strains were isolated from poultry litter samples. C. lari and C. upsaliensis strains were isolated from canine feces.

C. lari, C. upsaliensis, C. fetus, H. hepaticus, A. cryaerophilus, and A. butzleri were cultured on blood agar in a microaerobic atmosphere containing approximately 5% O2, 10% CO2, and 85% N2, generated with the Pack-Campylo Anaeropack system (Mitsubishi Gas Chemical Company, Inc., New York, N.Y.), at 37°C for 36 h. Bacterial colonies were scraped off the plates, and the DNA template for PCR was prepared by the boiling method (36).

Statistics.
The kappa test and chi-square test were performed to evaluate the correlation between cultural and PCR-ELISA methods for detecting S. enterica, C. jejuni, and C. coli. Sensitivity and specificity were also calculated from the contingency table generated for the kappa and chi-square tests. StatXact-3 version 3.0 (Cytel Software Corporation, Cambridge, Mass.) was used to perform statistical analysis.


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RESULTS
 
PCR-ELISA to detect Campylobacter sp., C. jejuni, and C. coli on chicken carcasses.
We determined the specificity of the PCR-ELISA for C. jejuni (n = 12), C. coli (n = 6), C. lari (n = 2), C. upsaliensis (n = 1), C. fetus (n = 1), E. coli (n = 6), Salmonella (n = 5), H. hepaticus (n = 1), A. cryaerophilus (n = 1), A. butzleri (n = 1), C. freundii (n = 1), K. pneumoniae (n = 1), S. fonticola (n = 1), and E. cloacae (n = 1). The PCR-ELISA values for OD at 405 nm (OD405) for the negative control strains (non-C. jejuni-C. coli) (OD405 range, 0.162 to 0.235) were recorded and served as the cutoff point for identifying positives. They were the mean plus 2 (cutoff point, 0.22) or 4 (cutoff point, 0.26) standard deviations. Any reaction with its OD405 between 0.22 and 0.26 was judged as weakly positive while those with OD405s greater than 0.26 were considered strongly positive. The OD405 range for C. jejuni (n = 12) and C. coli (n = 6) was between 0.26 and 4.0.

In developing a PCR-ELISA, we envisioned a test that could detect the target pathogen directly from the sample, without a preenrichment step. It was therefore necessary to optimize the PCR to detect the fewest number of cells possible per reaction. One PCR parameter that greatly influences sensitivity is cycle number; increasing the number of cycles increases the sensitivity of the PCR (45). PCR-ELISA was conducted on 10-fold serial dilutions of DNA template with a PCR program of 25, 30, and 40 cycles. Studies were done in triplicate. OD405s were recorded, averaged, and plotted against cell number (Fig. 1). For 40 PCR cycles, the reflection point (OD versus cell density) was 4.0 x 102 CFU/ml, while with 30 and 25 cycles of PCR amplification, the reflection points were 4.0 x 106 and 4.0 x 107 CFU/ml, respectively. The detection limit of PCR-ELISA on Campylobacter sp. was determined to be as low as 346 fg, or the equivalent of 40 CFU/ml for 40 PCR cycles. There was a linear correlation (R = 0.987; P < 0.001) between OD405 and cell density for 40 PCR cycles with the minimum limit of detection at 40 CFU/ml. For cell densities of >106 CFU/ml, it was necessary to dilute the sample 10-fold in order for the signal to fall within the linear detection range of PCR-ELISA and to estimate campylobacter cell numbers by this method.



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  		FIG. 1. Correlation between Campylobacter PCR-ELISA OD405s and Campylobacter cell count and the sensitivity of PCR in detecting C. coli or C. jejuni as a function of the number of PCR cycles. Arrows point to the reflection points (log CFU per milliliter) where the log-linear phase of OD readings begins for different cycles of PCR amplification.

Next, we applied our PCR-ELISA toward the detection and enumeration of C. coli and C. jejuni directly from carcass rinse. Thirty-two chicken carcass rinses were cultured under microaerobic conditions for detection of Campylobacter spp. DNA was also extracted from the same carcass rinses for PCR-ELISA. All the samples were Campylobacter positive based on the culturing method, with the highest Campylobacter cell count at 3.16 x 104 CFU/ml and the lowest Campylobacter cell count at 5 CFU/ml. With PCR-ELISA, 31 of 32 samples were PCR positive for Campylobacter. Among them, there were 4 weak positives (OD405, 0.22 to 0.255) and 27 strong positives (OD405, 0.28 to 2.2). There was one false negative (3.1%). There was a linear correlation between PCR-ELISA OD and colony counts (R = 0.792; P < 0.001) (Fig. 2A).



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  		FIG. 2. Linear correlation between PCR-ELISA OD405s and bacterial cell density as determined by traditional plate count. (A) PCR-ELISA OD405s versus C. coli or C. jejuni cell concentration in chicken carcass rinses (Pearson correlation, P < 0.001). (B) PCR-ELISA OD405s versus Salmonella cell concentration in samples (Pearson correlation, P < 0.001).

PCR-ELISA to detect S. enterica present in poultry farm drag swabs and chicken carcass rinses.
To determine the specificity of the probes, we performed PCR-ELISA on S. enterica serotypes Typhimurium (n = 4), Enteritidis (n = 3), Hadar (n = 2), Heidelberg (n = 2), Kentucky (n = 2), Agona (n = 1), Anatum (n = 1), Bredney (n = 1), Chester (n = 1), Choleraesuis (n = 1), Infantis (n = 1), Indiana (n = 1), Give (n = 1), London (n = 1), Montevideo (n = 1), Mbandaka (n = 1), Muenchen (n = 1), Ohio (n = 1), Schwarzengrund (n = 1), and St-Paul (n = 1) and on other members of the Enterobacteriaceae, including Citrobacter freundii (n = 1), Klebsiella pneumoniae (n = 1), Serratia fonticola (n = 1), Enterobacter cloacae (n = 1), and Escherichia coli (n = 10). PCR-ELISA OD405s for negative control strains (non-Salmonella) were recorded (OD405 range, 0.205 to 0.418) and served as the cutoff point for identifying positives. They were the mean plus 2 (cutoff point, 0.48) or 4 (cutoff point, 0.67) standard deviations. Any reaction with an OD405 above 0.48 was considered weakly positive, and any with an OD405 above 0.67 was recorded as strongly positive. Of the 20 different S. enterica serotypes examined, all were positive by PCR-ELISA with OD values of >=0.67. The OD405 range for Salmonella (n = 20) was 0.67 to 4.0. The detection limit of PCR-ELISA was found to be 2 x 102 CFU/ml for 40 PCR cycles. There was also a linear correlation (R = 0.924; P < 0.001) between OD405 and cell density (Fig. 2B).

This molecular biology-based method was first applied as a screen for Salmonella in overnight TBG enrichments (n = 60) of drag swabs. Thirty samples were culture positive for Salmonella, while PCR-ELISA identified 33 samples positive for Salmonella (OD405 range, 0.528 to 3.983) (Table 2). Of the samples positive for Salmonella by only one method, one was culture positive but PCR-ELISA negative and four were PCR-ELISA positive but culture negative (relative sensitivity, 0.97; relative specificity, 0.87). There was excellent correlation between the two methods as evidenced by the kappa test ({kappa} = 0.83) and the chi-square test (P < 0.001).


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  		TABLE 2. Comparison of PCR-ELISA with culture method for detecting S. enterica, C. coli, and C. jejuni on poultry or in the poultry production environment

We then directly screened 120 chicken carcass rinses for Salmonella by PCR-ELISA, of which 30 samples were culture positive. The PCR-ELISA identified 26 positive samples directly from carcass rinses, without an overnight preenrichment step. Seventeen of these samples yielded strongly positive reactions, with their OD405 readings being >0.67. However, some discrepancies were found between the culture and PCR-ELISA results. Ten samples were culture positive but ELISA negative. Another six samples were PCR-ELISA positive but culture negative. The PCR-ELISA for Salmonella yielded 5.0% false positives and 8.3% false negatives based on the assumption that the cultural method is the standard for detection (relative sensitivity, 0.67; relative specificity, 0.93) (Table 2). The chi-square test indicated a significant correlation between PCR-ELISA and culture methods for detecting Salmonella (P < 0.001), while there was good agreement according to the kappa test (0.63). Based on OD405s (range, 0.489 to 2.657), the estimated level of Salmonella contamination was between 2 x 102 and 2 x 104 CFU/ml for the carcass rinse.


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DISCUSSION
 
Conventional cultural methods for isolating Campylobacter and Salmonella are time-consuming and labor-intensive, especially when a large number of samples are tested. This study evaluated PCR-ELISA as an alternative to a cultural detection method for C. jejuni, C. coli, and S. enterica. The lipoprotein-encoding gene ceuE had been successfully used as the target for specific identification of certain Campylobacter species (23). However, the relative sensitivity and specificity for the identification of C. jejuni are 0.88 and 0.98, respectively, by use of these ceu-based PCR primers (40). We therefore designed our PCR primers and capture probes to a region of ceuE conserved between Campylobacter species C. coli and C. jejuni in order to reduce the chance of false negatives due to sequence divergence in ceuE among C. coli and C. jejuni isolates. The PCR-ELISA was specific to C. coli and C. jejuni. With our primers and capture probe, PCR-ELISA was also sensitive enough to detect campylobacters C. coli and C. jejuni in carcass rinse at levels as low as 40 CFU/ml. Salis et al. indicated that their PCR-ELISA could detect one Campylobacter cell per reaction but only with purified DNA template (46). PCR-ELISA has been applied toward the detection of Campylobacter species from poultry (41), clinical samples (32), and environmental waters (48), but few studies have evaluated the utility of PCR-ELISA for detecting Campylobacter directly from samples. Lawson et al. reported using PCR-ELISA to detect Campylobacter directly from fecal samples (32). Though their probes could identify other Campylobacter species, they obtained 12.9% false positives and 9.6% false negatives for C. jejuni and C. coli. The one false negative reported in this study was due to campylobacter cell density, at 5 CFU/ml, being below the minimum detection limit of the PCR-ELISA.

In this study, we were also able to detect Salmonella present in chicken carcass rinses and broth enrichments by PCR-ELISA. ELISA has been reported previously to increase both the sensitivity and the specificity of PCR over those of gel electrophoresis-based PCR assays (32, 41). PCR-ELISA avoids possible subjective interpretations in PCR due to "nonspecific products" or "bands of unknown origin" (60). To our knowledge, the only study to evaluate the utility of this technique in detecting Salmonella was conducted by Luk et al. (35). They developed PCR-ELISA to detect the amplified lipopolysaccharide rfbS gene as a means for rapid screening of serogroup D Salmonella in stool specimens. The test is specific for group D Salmonella with a detection limit of 10 bacteria per reaction when pure bacterial cultures are used. PCR-ELISA in our study increased sensitivity by 1,000-fold (40 CFU/ml) for Campylobacter and 100-fold (2 x 102 CFU/ml) for Salmonella over that of gel-based PCR. This is in agreement with the studies of Lawson et al. and O'Sullivan et al. (32, 41).

The false negatives associated with Salmonella-specific PCR-ELISA were not due to divergence among the target sequence, invA, PCR primers, and ELISA capture probe since Salmonella bacteria cultured from those samples were PCR-ELISA positive. The false negatives may be due to differences in sensitivity of the two methods, PCR-ELISA (>=102 CFU/ml) and culture (1 CFU/ml) (34). This difference in the sensitivity between PCR and culture may be due in part to volume constraints, since the sample volumes that can be processed by culture methods are larger than those for PCR (milliliters versus microliters) (32, 34, 48). However, an enrichment step could circumvent this problem by bringing salmonella cell numbers into the detection range of PCR-ELISA (34). In fact, we observed a lower percentage of false positives for samples following the enrichment step in TBG (8.3 versus 1.7%). Other possible explanations for the failure of PCR-ELISA include the degradation of Salmonella DNA before template was extracted or the presence of PCR inhibitors, despite the efforts that had been made to remove these inhibitors with the DNA isolation and purification kit (32, 56). The false positives that we observed for the Salmonella-specific PCR-ELISA may reflect the existence of sublethally damaged Salmonella cells in the samples, which may be nonculturable on the medium or under the culture conditions used in this study, or the presence of salmonellae in the sample at levels below PCR-ELISA detection limit (52). A preenrichment step may be required to allow damaged cells time to recover (34). The discrepancies between PCR and culture results may also be attributed to differences in enrichment, differential-selective media, and culture conditions in isolating certain S. enterica serotypes (52) as well as the failure to recognize atypical Salmonella colony types on differential-selective agars (21, 52).

Unlike Salmonella, we were unable to apply the statistical comparison of culture with Campylobacter-specific PCR-ELISA due to the organism's prevalence in the samples tested. One can sometimes find a flock of commercial birds in house that are campylobacter negative by culture (53). However, these flocks are hard to find, especially at certain times of year. When these flocks are caught caged and transported in soiled coops, they can become exposed to Campylobacter (51). They can also become exposed at the plant during hanging and picking (9). It would be difficult, if not impossible, to be sure that a flock that tested negative on the commercial farm remains negative during processing. With growing evidence that Campylobacter is transmitted vertically from the breeder parents to broiler progeny, it will be equally difficult to experimentally raise campylobacter-free chickens for the purposes of validating this PCR-ELISA (16, 28, 44). We were therefore unable to determine if PCR-ELISA was comparable to culture due to the absence of campylobacter-free samples. In the future, statistical validation of this PCR-ELISA will require testing of samples in which there is an expectation that a certain number of the sample(s) will be Campylobacter free (31, 38).

The PCR-ELISA detection scheme in our study could be a valuable tool in screening large numbers of samples for important food-borne pathogens like Salmonella and Campylobacter. It is rapid and cost-effective, taking 7 h for performance of the PCR-ELISA with a cost of about $3/sample, starting from the DNA extraction. When Campylobacter and Salmonella were screened for, few false negatives were found by PCR-ELISA, and that therefore allows us to focus culture on PCR-positive samples. Another advantage of PCR-ELISA is its 96-well microplate format, which allows larger sample sizes to be analyzed at the same time and makes automation possible.

PCR-ELISA has been recently used to quantify specific pathogens from clinical samples (1, 6, 17). This method is easier than competitive PCR methods developed for detection and quantification of specific DNA targets since it allows rapid detection of amplicons without the need of gel electrophoresis (21, 35). With competitive PCR, the quantity of target template is achieved by initially determining a standard curve in which various known amounts of target DNA are coamplified with a fixed amount of internal standard, and the ratio of targets and insertion sequence amplicons are plotted against the input amount of target DNA. Specific target DNA in clinical samples can be quantified through interpolation from the standard curve following coamplification with the same amount of internal standard as that used to construct the standard curve. However, the reliance on gel electrophoresis and cumbersome quantification systems has impeded quantitative PCR as a diagnostic assay (6). In this study, we constructed a standard curve by plotting OD values against Campylobacter cell count for different PCR cycles. The log-linear phase of the curve can be used in a semiquantification of bacterial cell concentration (CFU per milliliter) in a sample by knowing its relevant OD in PCR-ELISA (22). This PCR-ELISA detection system can be further extended to perform quantitative analysis of bacterial contamination by including a competitive internal standard in the PCR. Serial control DNA dilutions can be coamplified with a fixed number of internal standards in separate experiments, and amplicons can be detected by PCR-ELISA. The standard curve can be constructed by plotting the mean OD ratios of control DNA to internal standard amplicons against the number of copies of serially diluted control DNA (6). Quantitative PCR-ELISA can be a potentially rapid detection method for the poultry industry. It may help in monitoring the contamination levels in processing plants and evaluating the performance of the hazard analysis and critical control point system. Quantitative data also facilitate setting up dose-response models in microbial risk assessment by which we can evaluate the impact of food-borne pathogens on human health (14, 26). However, the limitation of this PCR test as well as any PCR-based assay is its inherent inability to distinguish live from dead bacterial cells. Due to the short-half life of mRNA in the bacterial cell, RNA may serve as a better target template for PCR in development of rapid methods for detection and enumeration of live salmonellae or campylobacters on food products by PCR-ELISA (47, 55).


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ACKNOWLEDGMENTS
 
USDA NRICGP grant 99-35212-8680, USDA Formula Funds, and a State of Georgia veterinary medicine agricultural research grant supported the work of J.J.M. USDA NRICGP grant 01-35212-10877 supported the work of C.L.H.


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FOOTNOTES
 
* Corresponding author. Mailing address: Department of Avian Medicine, College of Veterinary Medicine, The University of Georgia, Athens, GA 30603. Phone: (706) 542-5071. Fax: (706) 542-5630. E-mail: jmaurer{at}vet.uga.edu. Back


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REFERENCES
 
    1
  1. Allen, R. D., P. E. Pellett, J. A. Stewart, and M. Koopmans. 1995. Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA. J. Clin. Microbiol. 33:725-728.[Abstract]
    2. 2
  2. Anonymous. 1996. Pathogen reduction: hazard analysis and critical control point (HACCP) system. Final rule. Fed. Regist. 61:38806-38989.
    3. 3
  3. Anonymous. 1993. Advisory Committee on the Microbiological Safety of Food. Interim report on Campylobacter. Her Majesty's Stationery Office, London, United Kingdom.
    4. 4
  4. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Short protocols in molecular biology, 2nd ed. Current protocols. John Wiley & Sons, Inc., New York, N.Y.
    5. 5
  5. Bailey, J. S. 1998. Detection of Salmonella cells within 24 to 26 hours in poultry samples with the polymerase chain reaction BAX system. J. Food Prot. 61:792-795.[Medline]
    6. 6
  6. Barber, L., J. J. Egan, A. J. Turner, M. Guiver, A. A. Woodcock, N. Yonan, A. K. Deiraniya, and A. J. Fox. 1999. The development of a quantitative PCR ELISA to determine HCMV DNAaemia levels in heart, heart/lung and lung transplant recipients. J. Virol. Methods 82:85-97.[CrossRef][Medline]
    7. 7
  7. Bayardelle, P., and M. Zafarullah. 2002. Development of oligonucleotide primers for the specific PCR-based detection of the most frequent Enterobacteriaceae species DNA using wec gene templates. Can. J. Microbiol. 48:113-122.[CrossRef][Medline]
    8. 8
  8. Berrang, M. E., R. J. Buhr, and J. A. Cason. 2000. Campylobacter recovery from external and internal organs of commercial broiler carcass prior to scalding. Poult. Sci. 79:286-290.[Abstract/Free Full Text]
    9. 9
  9. Berrang, M. E., R. J. Buhr, J. A. Cason, and J. A. Dickens. 2001. Broiler carcass contamination with Campylobacter from feces during defeathering. J. Food Prot. 64:2063-2066.[Medline]
    10. 10
  10. Blankenship, L. C., J. S. Bailey, N. A. Cox, N. J. Stern, R. Brewer, and O. Williams. 1993. Two-step mucosal competitive exclusion flora treatment to diminish salmonellae in commercial broiler chickens. Poult. Sci. 72:1667-1672.[Medline]
    11. 11
  11. Blaser, M. J. 1997. Epidemiological and clinical features of Campylobacter jejuni infection. J. Infect. Dis. 176(Suppl. 2):S103-S105.
    12. 12
  12. Boyd, E. F., J. Li, H. Ochman, and R. K. Selander. 1997. Comparative genetics of the inv-spa invasion gene complex of Salmonella enterica. J. Bacteriol. 179:1985-1991.[Abstract/Free Full Text]
    13. 13
  13. Byrd, J. A., D. E. Corrier, J. R. Deloach, and D. J. Nisbet. 1997. Comparison of drag-swab environmental protocols for the isolation of Salmonella in poultry houses. Avian Dis. 41:709-713.[CrossRef][Medline]
    14. 14
  14. Coleman, M., and H. Marks. 1998. Topics in dose-response modeling. J. Food Prot. 61:1550-1559.[Medline]
    15. 15
  15. Corry, J. E., and H. I. Atabay. 2001. Poultry as a source of Campylobacter and related organisms. Symp. Ser. Soc. Appl. Microbiol. 30:96S-114S.
    16. 16
  16. Cox, N. A., N. J. Stern, K. L. Hiett, and M. E. Berrang. 2002. Identification of a new source of Campylobacter contamination in poultry: transmission from breeder hens to broiler chickens. Avian Dis. 46:535-541.[CrossRef][Medline]
    17. 17
  17. Davison, E., R. Borrow, M. Guiver, E. B. Kaczmarski, and A. J. Fox. 1996. The adaptation of the IS1106 PCR to a PCR ELISA format for the diagnosis of meningococcal infection. Serodiagn. Immunother. Infect. Dis. 8:51-56.
    18. 18
  18. Day, W. A., I. L. Pepper, and L. A. Jones. 1997. Use of an arbitrarily primed PCR product in the development of a Campylobacter jejuni-specific PCR. Appl. Environ. Microbiol. 63:1019-1023.[Abstract]
    19. 19
  19. Dickens, J. A., N. A. Cox, J. S. Bailey, and J. E. Thomson. 1985. Automated microbiological sampling of broiler carcasses. Poult. Sci. 64:1116-1120.
    20. 20
  20. Endtz, H. P., G. J. Ruijs, A. H. Zwinderman, T. van der Reijden, M. Biever, and R. P. Mouton. 1991. Comparison of six media, including a semisolid agar, for the isolation of various Campylobacter species from stool specimens. J. Clin. Microbiol. 29:1007-1010.[Abstract/Free Full Text]
    21. 21
  21. Feder, I., J. C. Nietfeld, J. Galland, T. Yearly, J. M. Sargeant, R. Oberst, M. L. Tamplin, and J. B. Luchansky. 2001. Comparison of cultivation and PCR-hybridization for detection of Salmonella in porcine fecal and water samples. J. Clin. Microbiol. 39:2477-2484.[Abstract/Free Full Text]
    22. 22
  22. Ferre, F. 1992. Quantitative or semi-quantitative PCR: reality versus myth. PCR Methods Appl. 2:1-9.[Medline]
    23. 23
  23. Gonzalez, I., K. A. Grant, P. T. Richardson, S. F. Park, and M. D. Collins. 1997. Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant. J. Clin. Microbiol. 35:759-763.[Abstract]
    24. 24
  24. Grennan, B., N. A. O'Sullivan, R. Fallon, C. Carroll, T. Smith, M. Glennon, and M. Maher. 2001. PCR-ELISA for the detection of Campylobacter jejuni and Campylobacter coli in poultry samples. BioTechniques 30:602-606, 608-610.[Medline]
    25. 25
  25. Hajna, A. A., and S. R. Damon. 1956. New enrichment and plating media for the isolation of Salmonella and Shigella organisms. Appl. Microbiol. 4:341-345.
    26. 26
  26. Holcomb, D. L., M. A. Smith, G. O. Ware, Y. C. Hung, R. E. Brackett, and M. P. Doyle. 1999. Comparison of six dose-response models for use with foodborne pathogens. Risk Anal. 19:1091-1100.[Medline]
    27. 27
  27. Ingraham, J. L., O. Maaløe, and F. C. Neidhardt. 1983. Growth of the bacterial cell. Sinauer Associates, Sunderland, Mass.
    28. 28
  28. Jacobs-Reitsma, W. F. 1995. Campylobacter bacteria in breeder flocks. Avian Dis. 39:355-359.[CrossRef][Medline]
    29. 29
  29. Koch, W. H., W. L. Payne, B. A. Wentz, and T. A. Cebula. 1993. Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods. Appl. Environ. Microbiol. 59:556-560.[Abstract/Free Full Text]
    30. 30
  30. Konkel, M. E., S. A. Gray, B. J. Kim, S. G. Gravis, and J. Yoon. 1999. Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J. Clin. Microbiol. 37:510-517.[Abstract/Free Full Text]
    31. 31
  31. Kulkarni, S. P., S. Lever, J. M. Logan, A. J. Lawson, J. Stanley, and M. S. Shafi. 2002. Detection of Campylobacter species: a comparison of culture and polymerase chain reaction based methods. J. Clin. Pathol. 55:749-753.[Abstract/Free Full Text]
    32. 32
  32. Lawson, A. J., J. M. J. Logan, G. L. O'Neill, M. Desal, and J. Stanley. 1999. Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay. J. Clin. Microbiol. 37:3860-3864.[Abstract/Free Full Text]
    33. 33
  33. Lazar, J. G. 1995. Immunological detection of PCR products, p. 177-202. In C. W. Dieffenbach and G. S. Dveksler (ed.), PCR primer: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
    34. 34
  34. Liu, T., K. Liljebjelke, E. Bartlett, C. Hofacre, S. Sanchez, and J. J. Maurer. 2002. Application of nested polymerase chain reaction to detection of Salmonella in poultry environment. J. Food Prot. 65:1227-1232.[Medline]
    35. 35
  35. Luk, J. M., U. Kongmuang, R. S. W. Tsang, and A. A. Lindberg. 1997. An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D salmonellae: a rapid screening prototype. J. Clin. Microbiol. 35:714-718.[Abstract]
    36. 36
  36. Martin, C. S., L. Butler, and I. Bronstein. 1995. Quantitation of PCR products with chemiluminescence. BioTechniques 18:908-913.[Medline]
    37. 37
  37. Mead, P. S., L. Slutsker, V. Dietz, L. F. McCaig, J. S. Bresee, C. Shapiro, P. M. Griffin, and R. V. Tauxe. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5:607-625.[Medline]
    38. 38
  38. Moreno, Y., S. Botella, J. L. Alonso, M. A. Ferrus, M. Hernandez, and J. Hernandez. 2003. Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization. Appl. Environ. Microbiol. 69:1181-1186.[Abstract/Free Full Text]
    39. 39
  39. Notermans, S., M. H. Zwietering, and G. C. Mead. 1994. The HACCP concept: identification of potentially hazardous microorganisms. Food Microbiol. 11:203-214.
    40. 40
  40. On, S. L., and P. J. Jordan. 2003. Evaluation of 11 PCR assays for species-level identification of Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 41:330-336.[Abstract/Free Full Text]
    41. 41
  41. O'Sullivan, N. A., R. Fallon, C. Carroll, T. Smith, and M. Maher. 2000. Detection and differentiation of Campylobacter jejuni and Campylobacter coli in broiler chicken samples using a PCR/DNA probe membrane based colorimetric detection assay. Mol. Cell. Probes 14:7-16.[CrossRef][Medline]
    42. 42
  42. Park, S. F., and P. T. Richardson. 1995. Molecular characterization of a Campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins. J. Bacteriol. 177:2259-2264.[Abstract/Free Full Text]
    43. 43
  43. Patel, J. B. 2001. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol. Diagn. 6:313-321.[CrossRef][Medline]
    44. 44
  44. Pearson, A. D., M. H. Greenwood, R. K. Feltham, T. D. Healing, J. Donaldson, D. M. Jones, and R. R. Colwell. 1996. Microbial ecology of Campylobacter jejuni in a United Kingdom chicken supply chain: intermittent common source, vertical transmission, and amplification by flock propagation. Appl. Environ. Microbiol. 62:4614-4620.[Abstract]
    45. 45
  45. Rameckers, J., S. Hummel, and B. Herrmann. 1997. How many cycles does a PCR need? Determinations of cycle numbers depending on the number of targets and the reaction efficiency factor. Naturwissenschaften 84:259-262.[CrossRef][Medline]
    46. 46
  46. Salis, A. D., A. J. Fox, F. J. Bolton, D. R. A. Wareing, D. L. A. Greenway, and R. Borrow. 2001. Development of a PCR ELISA assay for the identification of Campylobacter jejuni and Campylobacter coli. Mol. Cell. Probes 15:291-300.[CrossRef][Medline]
    47. 47
  47. Salis, A. D., F. J. Bolton, A. J. Fox, D. R. A. Wareing, and D. L. A. Greenway. 1998. A reverse transcriptase polymerase chain reaction assay for the detection of thermophilic Campylobacter spp. Mol. Cell. Probes 12:317-322.[CrossRef][Medline]
    48. 48
  48. Salis, A. D., F. J. Bolton, A. J. Fox, D. R. A. Wareing, and D. L. A. Greenway. 2002. Detection of Campylobacter jejuni and Campylobacter coli in environmental waters by PCR enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 68:1319-1324.[Abstract/Free Full Text]
    49. 49
  49. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
    50. 50
  50. Savic, B., U. Sjobring, S. Alugupalli, L. Larson, and H. Miorner. 1992. Evaluation of the polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum. J. Infect. Dis. 166:1177-1180.[Medline]
    51. 51
  51. Slader, J., G. Domingue, F. Jorgensen, K. McAlpine, R. J. Owen, F. J. Bolton, and T. J. Humphrey. 2002. Impact of transport crate reuse and of catching and processing on Campylobacter and Salmonella contamination of broiler chickens. Appl. Environ. Microbiol. 68:713-719.[Abstract/Free Full Text]
    52. 52
  52. Soumet, C., G. Ermel, V. Rose, N. Rose, P. Drouin, G. Salvat, and P. Colin. 1999. Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses. Lett. Appl. Microbiol. 29:1-6.[CrossRef][Medline]
    53. 53
  53. Stern, N. J., P. Fedorka-Cray, J. S. Bailey, N. A. Cox, S. E. Craven, K. L. Hiett, M. T. Musgrove, S. Ladely, D. Cosby, and G. C. Mead. 2001. Distribution of Campylobacter spp. in selected U.S. poultry production and processing operations. J. Food Prot. 64:1705-1710.[Medline]
    54. 54
  54. Stern, N. J., K. Wojton, and B. Kwiatek. 1992. A differential selective medium and dry ice generated atmosphere for recovery of Campylobacter jejuni. J. Food Prot. 55:514-517.
    55. 55
  55. Szabo, E. A., and B. M. Mackey. 1999. Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR). Int. J. Food Microbiol. 51:113-122.[CrossRef][Medline]
    56. 56
  56. Tietjen, M., and D. Y. C. Fung. 1995. Salmonella and food safety. Crit. Rev. Microbiol. 21:53-83.[Medline]
    57. 57
  57. van Eys, G. J. J. M., C. Gravekamp, M. J. Gerritsen, W. Quint, M. T. E. Cornelissen, J. Ter Schegget, and W. J. Terpstra. 1989. Detection of leptospires in urine by polymerase chain reaction. J. Clin. Microbiol. 27:2258-2262.[Abstract/Free Full Text]
    58. 58
  58. van Zwet, A. A., J. C. Thijis, A. M. Kooistra-Smid, J. Schirm, and J. A. Snijder. 1994. Use of PCR with feces for detection of Helicobacter pylori infections in patients. J. Clin. Microbiol. 32:1346-1348.[Abstract/Free Full Text]
    59. 59
  59. Waltman, W. D., A. Horne, C. Pirkle, and T. Dickson. 1991. Use of delayed secondary enrichment for the isolation of Salmonella in poultry and poultry environments. Avian Dis. 35:88-92.[CrossRef][Medline]
    60. 60
  60. Winters, D. K., and M. F. Slavik. 1995. Evaluation of a PCR based assay for the specific detection of Campylobacter jejuni in chicken washes. Mol. Cell. Probes 9:307-310.[CrossRef][Medline]
    61. 61
  61. Wittwer, C. T., G. C. Fillmore, and D. R. Hillyard. 1989. Automated polymerase chain reaction capillary tubes with hot air. Nucleic Acids Res. 17:4353-4357.[Abstract/Free Full Text]
    62. 62
  62. Yang, Y. J., M. C. Huang, S. M. Wang, J. J. Wu, C. P. Cheng, and C. C. Liu. 2002. Analysis of risk factors for bacteremia in children with nontyphoidal Salmonella gastroenteritis. Eur. J. Clin. Microbiol. Infect. Dis. 21:290-293.[CrossRef][Medline]
    63. 63
  63. Zhao, C., B. Ge, J. De Villena, R. Sudler, E. Yeh, S. Zhao, D. G. White, D. Wagner, and J. Meng. 2001. Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the greater Washington, D.C., area. Appl. Environ. Microbiol. 67:5431-5436.[Abstract/Free Full Text]

Applied and Environmental Microbiology, June 2003, p. 3492-3499, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3492-3499.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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