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Previous Article | Next Article 
Applied and Environmental Microbiology, April 2005, p. 2183-2185, Vol. 71, No. 4
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.4.2183-2185.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
| SHORT REPORT |
k Prokop,2
Yukari Sato,1
Petr Jerabek,2
Ashwani Kumar,3
Yoshiyuki Ohtsubo,1
Masataka Tsuda,1 and
Ji
í Damborsk
2
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan,1 National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic,2 Industrial Toxicology Research Center, Lucknow, India3
Received 23 August 2004/ Accepted 22 October 2004
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ABSTRACT |
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-, ß-, 
-, and 
-isomers of HCH and causes serious environmental pollution problems. We demonstrate here that the haloalkane dehalogenase LinB, reported earlier to mediate the second step in the degradation of -HCH in Sphingomonas paucimobilis UT26, metabolizes ß-HCH to produce 2,3,4,5,6-pentachlorocyclohexanol. |
INTRODUCTION |
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-, ß-, -, and -isomers has been used worldwide. Many countries, however, have now prohibited its use because of its toxicity and persistence in upland soil, but several contaminated sites remain throughout the world. Among these isomers, ß-HCH is the most recalcitrant in the environment due to its chemical stability (1). Bacterial strains that degrade ß-HCH have been reported (6, 20), but the enzyme involved in the degradation process remains unknown.
Sphingomonas paucimobilis UT26 utilizes -HCH as a sole source of carbon and energy under aerobic conditions (3). The degradation pathway of -HCH in this bacterium includes two steps of dehydrochlorination, two steps of hydrolytic dehalogenation, and one dehydrogenation step, catalyzed by LinA, LinB, and LinC, respectively, leading to the formation of 2,5-dichlorohydroquinone, which undergoes further degradation (16). The enzyme -HCH dehydrochlorinase LinA mediates the metabolism of -, -, and -HCH but not that of the ß-isomer (11). We demonstrate here that UT26 is able to transform ß-HCH and that the activity is derived from the haloalkane dehalogenase LinB.
The activity of S. paucimobilis UT26 and its spontaneous linA deletion mutant YO5 (15) was assayed for the degradation of t-HCH (India Pesticides, Lucknow, India), which consists of (67.4%), ß (6.8%), (17.3%), and (7.4%)-isomers of HCH. Briefly, a small amount of a bacterial colony, grown on 1/3 Luria broth-agar medium (7) at 30°C, was picked and suspended (about 20 mg of cells/ml, final concentration) in the assay solution, 17 µM t-HCH in W medium (3). After incubation at 30°C for 12 h, the assay solution was extracted with an equal volume of ethyl acetate and analyzed by a Shimadzu GC-17A gas chromatograph (GC) equipped with an electron capture 63Ni detector (Shimadzu, Kyoto, Japan) and an Rtx-1 capillary column (30 m by 0.25 mm by 0.25 µm; Restek). The column temperature was increased from 100 to 260°C at a rate of 20°C/min, and the gas flow rate was 30 ml/min. During the period of incubation, UT26 completely degraded all four HCH isomers, while YO5 degraded only ß-HCH (data not shown). One unit of ß-HCH degradation activity was defined as the activity required for the transformation of 1 µmol of ß-HCH per min. The activity was calculated using linear range in the first several hours of reaction. When W medium containing 17 µM ß-HCH was used as the assay medium, UT26 and YO5 cells degraded ß-HCH linearly during the first 6 h at a rate of 1.5 x 10–6 and 1.6 x 10–6 U/mg of cells, respectively. No apparent difference in activity levels between these two strains suggested that an enzyme other than LinA is responsible for the metabolism of ß-HCH. This observation is consistent with LinA's recognition of the trans and diaxial pairs of chlorine and hydrogen (9, 23), which are not present in ß-HCH. In an earlier report, no degradation of ß-HCH by UT26 was observed (6). We, however, could detect the very low activity in this study because a high concentration of cells was used for the reaction.
In our search for a gene responsible for the degradation of ß-HCH, Pseudomonas putida PpY101 strains harboring cosmids pKSR1 (4), pKSR401 (10), pKSR501 (12), and pKSM1920 (8), containing the linA, linB, linC, and linRED operons, respectively, were assayed for their potential to degrade ß-HCH. To our surprise, the strain P. putida PpY101(pKSR401), containing the linB gene, showed ß-HCH degradation activity (3.1 x 10–7 U/mg of cells in the first 3 h). The activity for ß-HCH degradation was further assayed in two more strains. The strain Escherichia coli DH5(pULBH6) expressing the His-tagged LinB protein (14) showed ß-HCH degradation activity (1.1 x 10–6 U/mg of cells in the first 3 h). On the other hand, S. paucimobilis UT26DB, whose linB gene had been disrupted by insertion of the kanamycin cassette that originated from pUC4K (22), did not show the activity. These results clearly indicate that LinB has ß-HCH degradation activity.
LinB is a haloalkane dehalogenase of the /ß-hydrolase family of enzymes with relatively broad substrate specificity (5, 13). It has been the subject of crystallographic (17, 21), kinetic (19), mutagenesis (2), and computational (18) studies. In the present study, we evaluated the ß-HCH transformation by the purified His-tagged LinB protein (14). The reaction was initiated by the addition of enzyme (20 mg/liter, final concentration) to the reaction mixture containing 2 µM ß-HCH in 50 mM phosphate buffer (pH 7.5) at 37°C and was stopped by the addition of H2SO4 after 1 to 100 min. The hexane-extracted samples were analyzed on a GC equipped with a ZB-5 capillary column (30 m by 0.25 mm by 0.25 µm; Phenomenex) and an electron capture detector. The temperature program was isothermal at 60°C for 1 min, followed by an increase to 280°C at 20°C/min, and the carrier gas (He) flow rate was 1.8 ml/min. With the addition of LinB, ß-HCH was decreased with a simultaneous increase in a metabolite (Fig. 1A), tentatively designated M1. The Michaelis-Menten and product inhibition equations were fitted by numerical integration to a progress curve obtained from the kinetic experiment by using MicroMath Scientist (MicroMath Research). The best fit was obtained using the product inhibition equation (Fig. 1B). This suggests that LinB can bind the product, which competes with the substrate for an enzyme active site. The unique values of kcat and Km could not be calculated from the data because of the limited solubility of ß-HCH in water. Since the ß-HCH concentration was lower than Km, the substrate hydrolysis followed a first-order process. A first-order equation was fitted to the kinetic data (R2 = 0.997) by using Origin 6.1 (OriginLab Corp.). The first-order rate constant for ß-HCH decay, 0.041 ± 0.001 min–1, and the kcat/Km ratio, 0.001 s–1 · µM–1, have been estimated. The kcat/Km ratio for ß-HCH conversion by LinB is 2 orders of magnitude lower than that of the reaction of LinB with the best substrate 1-chlorohexane (kcat/Km = 0.16 s–1 · µM–1).
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In UT26, PCHL was not degraded even after its incubation for up to 2 days, indicating that the metabolism of ß-HCH is restricted to the formation of this metabolite. PCHL has lower hydrophobicity and lower chemical stability than ß-HCH, and the bacteria that degrade and utilize it may exist in the polluted environment, allowing the complete degradation of ß-HCH by a combination of biological pathways.
In summary, it was concluded that, in the bacterial strain S. paucimobilis UT26, haloalkane dehalogenase LinB converts ß-HCH to PCHL. This finding is extremely important, as it provides the first information regarding an enzyme to be used for metabolism of ß-HCH, which is one of the most recalcitrant environmental pollutants. LinB is possibly also involved in the degradation of ß-HCH in other reported bacteria (6, 20). Further experiments are necessary, however, to confirm the role of linB in S. paucimobilis strain B90, which seems to have stronger ß-HCH degradation activity than UT26 (6).
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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REFERENCES |
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-hexachlorocyclohexane (-BHC) by -BHC-assimilating Pseudomonas paucimobilis. Agric. Biol. Chem. 53:2015-2017.
-hexachlorocyclohexane. J. Bacteriol. 173:6811-6819.-hexachlorocyclohexane in Sphingomonas paucimobilis. J. Bacteriol. 181:6712-6719.-HCH degradation by Pseudomonas paucimobilis UT26. Chemosphere 26:1187-1201.[CrossRef]
-hexachlorocyclohexane (-HCH) in Pseudomonas paucimobilis. J. Bacteriol. 175:6403-6410.-hexachlorocyclohexane (-HCH) dehydrochlorinase (LinA) from Pseudomonas paucimobilis. Biosci. Biotechnol. Biochem. 57:1582-1583.
-hexachlorocyclohexane in Pseudomonas paucimobilis. J. Bacteriol. 176:3117-3125.-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. Appl. Environ. Microbiol. 63:3707-3710.[Abstract]
-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. Protein Expr. Purif. 17:299-304.[CrossRef][Medline]
-HCH degradation in Sphingomonas paucimobilis UT26, are localized in periplasmic space without molecular processing. J. Bacteriol. 181:5409-5413.-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. J. Ind. Microbiol. Biotechnol. 23:380-390.[CrossRef][Medline]
-hexachlorocyclohexane dehydrochlorinase LinA. J. Biol. Chem. 276:7734-7740.
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