Files in this Data Supplement:

• Supplemental file 1 - Effect of long stretches on relative signal intensity for 50mer or 70mer oligonucleotide probes (Fig. S1).
  Zipped TIF document, 2.5MB.
• Supplemental file 2 - 50mer and 70mer perfect match (PM) and mismatch (MM) oligonucleotide probe sequences as well as complementary targets for four 70mer PM probes (Table S1); comparison of theoretical probe design criteria and experimental hybridization behavior (Table S2); summary of parameters (probe-target identity, stretch, MMPD, and binding free energy) and their relationships with signal intensity and SNR for some probes (Table S3); and summary of parameter data for all PM and MM probes (Table S4)..
  MS Word document, 878K.