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Applied and Environmental Microbiology, August 2005, p. 4286-4296, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4286-4296.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Elucidation of a Carotenoid Biosynthesis Gene Cluster Encoding a Novel Enzyme, 2,2'-ß-Hydroxylase, from Brevundimonas sp. Strain SD212 and Combinatorial Biosynthesis of New or Rare Xanthophylls

Yasuhiro Nishida,1 Kyoko Adachi,2 Hiroaki Kasai,2 Yoshikazu Shizuri,2 Kazutoshi Shindo,3 Akiyoshi Sawabe,1 Sadao Komemushi,1 Wataru Miki,4 and Norihiko Misawa2*

School of Agriculture, Kinki University, Nakamachi, Nara,1 Marine Biotechnology Institute, Heita, Kamaishi, Iwate,2 Japan Women's University, Mejirodai, Bunkyo-ku, Tokyo,3 Suntory Limited, Shimamoto-cho, Mishima-gun, Japan4

Received 19 December 2004/ Accepted 3 March 2005


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ABSTRACT
 
A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2'-ß-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2'-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation.


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INTRODUCTION
 
Carotenoid pigments, which are included in a majority of vegetables, a variety of fruits, and a certain part of edible fish and shellfish, have attracted strong attention due to their beneficial effects on human health, e.g., their very likely prevention of chronic diseases such as cancer, cardiovascular ailments, and age-related macular degeneration (7, 12, 27, 36). A number of studies on carotenoids in relation to their health benefits, ranging from basic studies using experimental animals to clinical and epidemiological studies, have revealed that each carotenoid has characteristic individuality. For example, recent epidemiological studies have shown that ß-cryptoxanthin (3-hydroxy-ß-carotene), among dietary carotenoids, is associated with a reduced risk of lung cancer (24, 56), whereas supplemental ß-carotene has been observed to have either no effect or even a harmful effect on lung cancer risk (2, 24). Epidemiological and clinical studies have also shown that only lycopene, among dietary carotenoids, was inversely associated with prostate cancer risk (16, 51). Supplemental lutein and zeaxanthin are thought to protect against the development or progression of age-related macular degeneration and other eye diseases from the results of many studies (28, 45). Astaxanthin has been shown to inhibit the oxidation of low-density lipoprotein (20).

Xanthophylls are modified carotenes with various oxygen-containing functional groups, which constitute a major part of structurally and functionally diverse carotenoid pigments. More than 700 carotenoids have now been isolated from natural sources (8), and evaluating the pharmaceutical potential of various carotenoids with different structures could be an exciting field for medical researchers. However, the carotenoid species studied for this purpose so far have been restricted to a small number, including ß-carotene, {alpha}-carotene, and lycopene (carotenes) and lutein, zeaxanthin, ß-cryptoxanthin, canthaxanthin, and astaxanthin (xanthophylls). With the exception of those carotenoids, which can be isolated from a species of higher plants or be chemically synthesized, it has been difficult to find natural sources for supplying sufficient amounts of carotenoids. Metabolic engineering (combinatorial biosynthesis), using a variety of carotenoid biosynthesis genes, should be one of the most powerful methods to generate plenty of structurally diverse carotenoids (xanthophylls).

Since the first finding in 1990 that the catalytic functions of carotenoid biosynthetic gene products were able to be assigned by complementation analysis using Escherichia coli (30), a considerable number of carotenogenic genes have been cloned and functionally assigned (5, 32, 39, 42). Twenty-five carotenogenic genes, whose gene products have different catalytic functions, have been identified so far as necessary to synthesize different carotenoids, including crtU for ß-carotene desaturase, crtZ for ß-carotene hydoroxylase (3,3'-ß-hydroxylase), crtW (bkt) for ß-carotene ketolase (4,4'-ß-ketolase; 4,4'-ß-oxygenase), crtO (from Synechocystis sp. strain PCC6803) for ß-carotene mono-ketolase, and crtX for zeaxanthin glucosyltransferase. Functional expression of such genes in heterologous hosts such as E. coli has enabled us to engineer new pathways for producing carotenoids that are novel or rare in nature (40, 42). Further acquisition of new carotenoid biosynthesis genes, in particular a gene encoding a ß-ionone ring (ß-ring)-modifying enzyme, should contribute to the biotechnological synthesis of a variety of novel or rare xanthophylls.

The Marine Biotechnology Institute has isolated a number of marine bacteria which produce astaxanthin and its relevant ketocarotenoids (53-55) (http://www.mbio.jp/mbic). We have taxonomically reevaluated these bacteria and found that they fall into three genera of {alpha}-Proteobacteria, Brevundimonas sp., Paracoccus sp., and Erythrobacter sp. Two genes encoding ß-ring-modifying oxygenase enzymes, crtZ and crtW, have been isolated from Paracoccus sp. strain N81106 (MBIC01143), which was formerly classified as Agrobacterium aurantiacum (21), and Paracoccus sp. strain PC1 (MBIC03024), formerly classified as Alacaligenes sp. strain PC-1 (29), and the catalytic functions of the gene products, CrtZ and CrtW, have been identified (13, 15, 29, 31). However, there has been no report on the cloning of a carotenoid oxygenase gene from Brevundimonas sp. or Erythrobacter sp., except for one patent describing the cloning of crtW from Brevundimonas aurantiaca (PCT WO/02/079395). Brevundimonas sp. strain SD212 (MBIC03018), Paracoccus sp. strain N81106 (MBIC01143), and Erythrobacter sp. strain PC6 (MBIC02351) are, respectively, able to produce (2R,3S,3'S)-2-hydroxyastaxanthin (55), astaxanthin ß-D-glucoside (53), and (2R,3S,2'R,3'R)-4-ketonostoxanthin 3'-sulfate (54) in addition to astaxanthin. Judging from their chemical structures, the two bacterial genera Brevundimonas and Erythrobacter may possess a novel oxygenase gene encoding 2,2'-ß-hydroxylase in addition to two known oxygenase genes, crtZ and crtW. We report here the cloning and functional identification of a carotenoid biosynthesis gene cluster including the 2,2'-ß-hydroxylase gene that is structurally and functionally novel. We further show that combinatorial biosynthesis with the new gene enables the synthesis of a novel or rare carotenoid(s) with pharmaceutical potential which would otherwise be very difficult to chemically synthesize.


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MATERIALS AND METHODS
 
Bacterial strains, plasmids, and growth conditions.
The bacterial strains and the majority of plasmids used in this study are listed in Table 1. Brevundimonas sp. strain SD212 (Marine Biotechnology Institute culture collection number MBIC03018) was grown on marine broth 2216 (Difco) with shaking at 25°C. The Escherichia coli strains used were grown on the Luria-Bertani (LB) medium (38) with shaking at 37°C or 30°C. The media were supplemented when required with the following antibiotics at the indicated concentrations: ampicillin, 100 to 150 µg ml–1; chloramphenicol, 20 to 30 µg ml–1. A 1 mM concentration of IPTG (isopropyl-ß-D-thiogalactopyranoside) was used for inducing the E. coli JM109 transformants as required. The E. coli XL1-Blue MR and DH5{alpha} transformants were grown overnight at 37°C for the preparation of cosmid and plasmid DNA, respectively. The E. coli JM109 transformants were grown for 2 days at 30°C for pigment analysis.


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  		TABLE 1. Bacterial strains and plasmids used in this study

Recombinant DNA techniques.
The restriction enzymes, alkaline phosphatase (from E. coli C75), and DNA ligation kit were purchased from New England BioLabs, Takara Biochemicals, and Toyobo, respectively. DNA manipulation was conducted by standard methods (38) or as instructed by the suppliers. Plasmid or cosmid DNA was prepared with the QIAEX II purification kit (QIAGEN) or the automatic DNA isolation system PI-200 (Kurabo). PCR assay was carried out with an automated thermal cycler (Techne) using LA Taq polymerase (Takara Biochemicals). Colony hybridization and Southern hybridization were effected with Hybond N+ membranes and the Alkphos direct labeling and detection system (Amersham Pharmacia). The 1.1-kb crtI fragment of pCRTI-SD212 was used as the probe DNA for these hybridization experiments.

Construction of plasmids for accumulation of canthaxanthin and astaxanthin in E. coli.
Plasmids for the biosynthesis of canthaxanthin, pAC-Cantha, and astaxanthin, pAC-Asta, were constructed as follows. The Paracoccus sp. strain N81106 crtW gene, which was modified according to the codon usage of the Candida utilis GAP gene to reduce the GC content of the original gene (65%) (33), was used as the template for PCR amplification with primers H1437 (5'-GTC CCG AGA AGG AGG CTA GAT ATG TCC GCT CAC GCT TTG C-3'; the AvaI site is underlined) and H1438 (5'-CGG CGG CCG CCC GGG ACT AAG CGG TGT CAC CCT TGG TTC T-3'; the NotI site is underlined). An amplified 0.76-kb fragment was digested with AvaI and NotI. The Pantoea ananatis crtE gene (GenBank accession number D90087) was amplified from plasmid pCAR16 (30) by PCR using primers H1431 (5'-ATG CGG CCG CTT ATA AGG ACA GCC CGA ATG-3'; the NotI site is underlined) and H1432 (5'-CAG TCG ACA TCC TTA ACT GA CGG CAG CGA G-3'; the SalI site is underlined). An amplified 1.1-kb fragment was digested with NotI and SalI. The above 0.76-kb-AvaI-crtW-NotI and 1.1-kb NotI-crtE-SalI fragments were ligated with the larger fragment carrying P. ananatis crtB, crtI, and crtY, which had been obtained by digestion with AvaI and SalI from plasmid pACCAR16{Delta}crtX, to construct plasmid pAC-Cantha. Furthermore, both the 0.76-kb and 1.1-kb fragments were ligated with the larger fragment carrying P. ananatis crtB, crtI, crtY, and crtZ, which had been obtained by digestion with AvaI and SalI from plasmid pACCAR25{Delta}crtX, to construct plasmid pAC-Asta.

Preparation of genomic DNA and construction of cosmid library.
Total DNA was extracted from Brevundimonas sp. strain SD212 as previously described (49), partially digested with Sau3AI, and ligated into the BamHI site of SuperCos 1 (Stratagene). The ligates were packaged into bacteriophage {lambda} with a Lambda Inn packaging extract (Wako). E. coli XL1-Blue MR was infected with the resulting phages.

PCR amplification of crtI.
Well-conserved domains in the N-terminal region (FDAGPTV) and C-terminal region (GAGIPG) in the crtI genes were chosen for designing degenerate primers crtI-f (5'-TTY GAY GCI GGI CCI ACI GT-3') and crtI-r (5'-CCI GGR TGI GTI CCI GCI CC-3'). PCR amplification was performed in a 25-µl reaction mixture containing 100 ng of strain SD212 genomic DNA, each deoxynucleoside triphosphate (200 µM), primers (2.5 µM each), MgCl2 (2.5 mM), 1.0 U of LA Taq polymerase, and the buffer supplied with the enzyme. PCR assay was conducted as follows: preincubation at 95°C for 5 min; a total of 35 cycles at 95°C for 1 min, 50°C for 1 min, and 72°C for 1.5 min; and a final elongation step of 5 min at 72°C. An amplified 1.1-kb fragment was ligated into a pGEM-T Easy vector to construct plasmid pCRTI-SD212.

Nucleotide sequencing and computer analysis.
The nucleotide sequences of the inserted fragments of plasmids pCRTI-SD212, p5Bre2-15, and the others derived from p5Bre2-15 were determined in both orientations by a DNA sequencing kit (Big dye terminator cycle sequencing ready reaction kit, version 2; Perkin-Elmer) and a model 3700 DNA sequencer (Perkin-Elmer) according to the manufacturer's instructions. Homologous protein sequences in the protein sequence database were retrieved using version 2.0 of the BLAST program (3) and aligned with Clustal W, version 1.8 (18) and GeneDoc (35) (http://www.psc.edu/biomed/genedoc).

Construction of plasmids for expression of the individual SD212 crt genes or ORFs.
Plasmids for the expression of the individual crt genes or open reading frames (ORFs) of Brevundimonas sp. strain SD212 were constructed using plasmid p5Bre2-15 as the template for PCR amplification. Primers were designed to have the EcoRI site at the 5' end and the BamHI or XbaI site at the 3' end. The amplified fragments were digested with EcoRI and BamHI or XbaI and ligated with the EcoRI- and BamHI- or XbaI-digested pUC18. The primers for the construction of plasmid pUCBre-O11 were BO11-f (5'-TACGAA TTC GAT GTT GAG GGA TCT GCT CAT CA-3'; the EcoRI site is underlined) and BO11-r (5'-TAGAGG ATC CTC ACC GAA GAG GCG CTG AG-3'; the BamHI site is underlined).

HPLC analysis of carotenoid pigments accumulated in E. coli.
The cultured cells of E. coli JM109 transformants, which were obtained from a 2-ml culture, were extracted with 200 µl of acetone. The supernatant of the acetone extract was analyzed by high-performance liquid chromatography (HPLC) (Alliance; Waters) with a photodiode array detector (PDA) (model 2996; Waters). This was performed using a TSK gel ODS-80Ts column (4.6-mm inner diameter by 150 mm; Tosoh Co.), and the carotenoids were eluted at a flow rate of 1.0 ml min–1 with solvent A (methanol-water, 95:5) for 5 min, then by a linear gradient from solvent A to solvent B (methanol-tetrahydrofuran, 7:3) for 5 min, and finally by isocratic elution with solvent B for 8 min. HPLC analysis to identify lycopene and ß-carotene used an octadecyl silica reverse-phase Nova-pak HR 6-µm C18 column (3.9 by 300 mm; Waters) developed at a flow rate of 1 ml/min with acetonitrile-methanol-2-propanol (45:3:2 [vol/vol/vol]).

Purification and spectroscopic analysis of the carotenoid pigments.
The cultured cells of E. coli JM109 transformants were extracted with acetone-methanol (1:1). The concentrated extract was applied to a silica gel 60 column and progressively eluted with mixtures of hexane-ethyl acetate (8:2, 7:3, 6:4, and 1:1). Pigments 1 (0.05 mg), 2 (0.1 mg), and 3 (0.1 mg) were obtained from a 2-liter culture of E. coli(pUCBre-O11, pAC-Asta). Pigments 4 (0.5 mg), 5 (0.6 mg), and 6 (0.8 mg) were obtained from a 10-liter culture of E. coli(pUCBre-O11, pACCAR25{Delta}crtX). Pigments 7 (0.2 mg), 8 (0.4 mg), and 9 (0.4 mg) were obtained from a 10-liter culture of E. coli (pUCBre-O11, pAC-Cantha). Each pigment fraction was analyzed by HPLC/PDA/atmospheric pressure chemical ionization mass spectrometry (APCI-MS), nuclear magnetic resonance spectrometry (NMR), and high-resolution fast atom bombardment mass spectrometry (HRFABMS).

HPLC/PDA/APCI-MS was carried out with an LCQ Advantage ion trap mass spectrometer (ThermoFinnigan, San Jose, CA) connected to a NanoSpace SI-2 HPLC/PDA instrument (Shiseido, Tokyo, Japan). The positive-ion APCI mass spectra were acquired by scanning from m/z 200 to 800. HPLC separation was conducted in a Develosil C30-UG-3 column (1.0-mm inner diameter by 150 mm; Nomura Chemical Co., Seto, Japan) with gradient elution by a binary system consisting of solvent A (methanol-water, 96:4) and solvent B (tert-butyl methyl ether) at a flow rate of 0.1 ml/min. Isocratic elution by solvent A was used for the initial 12 min, followed by linear gradient elution to 60% (vol/vol) of solvent B for 60 min and then by isocratic elution with 60% of solvent B for a further 10 min.

NMR spectra were obtained with a Unity INOVA 750 instrument (Varian, Palo Alto, CA), using CDCl3 as the solvent. HRFABMS data were measured by a JMS700 instrument (Jeol, Tokyo, Japan), using m-nitro benzyl alcohol as a matrix.

Authentic samples of carotenoids were purchased from Sigma or Funakoshi or purified from Brevundimonas sp. strain SD212 or E. coli transformants expressing other bacterial crt genes.

Spectral data for individual carotenoids.
For pigment 1, (2R)-hydroxyastaxanthin: visible (VIS) {lambda}max 474 nm; LC/APCI-MS m/z 613 [M+ + H]; 1H NMR (750 MHz) {delta} 1.21 (3H, s), 1.26 (3H, s), 1.29 (3H, s), 1.32 (3H, s), 1.81 (1H, t, J = 13.0 Hz), 1.95 (6H, s), 1.98 to 2.00 (12H, s), 2.16 (1H, dd, J = 13.0, 5.5 Hz), 3.52 (1H, dd, J = 11.5, 2.0 Hz), 4.18 (1H, dd, J = 11.5, 2.5 Hz), 4.32 (1H, ddd, J = 13.0, 5.5, 2.0 Hz), 6.2 to 6.7 (14H, m). For pigment 2, astaxanthin: VIS {lambda}max 473 nm; LC/APCI-MS m/z 597 [M+ + H]. For pigment 3, adonixanthin: VIS {lambda}max 461 nm; LC/APCI-MS m/z 583 [M+ + H]. For pigment 4, (2R, 2'R)-dihydroxyzeaxanthin (nostoxanthin): VIS {lambda}max 449, 475 nm; LC/APCI-MS m/z 601 [M+ + H], 583 [M+ + H – H2O], 565 [M+ + H – 2H2O] (see Table 4 for 1H NMR data). For pigment 5, (2R)-hydroxyzeaxanthin (caloxanthin): VIS {lambda}max 450, 476 nm; LC/APCI-MS m/z 585 [M+ + H], 567 [M+ + H – H2O] (see Table 4 for 1H NMR data). For pigment 6, zeaxanthin: VIS {lambda}max 450, 476 nm; LC/APCI-MS m/z 569 [M+ + H]. For pigment 7, (2R, 2'R)-dihydroxycanthaxanthin [(2R, 2'R)-2, 2'-dihydroxy-ß,ß-carotene-4,4'-dione]: VIS {lambda}max 472 nm; LC/APCI-MS m/z 597 [M+ + H], 579 [M+ + H – H2O], 561 [M+ + H – 2H2O]; HRFABMS m/z [M+] (see Table 4 for 1H NMR data). Calculated: C40H32O4, 596.3866. Found: 596.3863. For pigment 8, (2R)-hydroxycanthaxanthin [(2R)-2-hydroxy-ß,ß-carotene-4,4'-dione]: VIS {lambda}max 474 nm; LC/APCI-MS m/z 581 [M+ + H], 563 [M+ + H – H2O]; HRFABMS m/z [M+] (see Table 4 for 1H NMR data). Calculated: C40H32O3, 580.3916. Found: 580.3900. For pigment 9, canthaxanthin: VIS {lambda}max 475 nm; LC/APCI-MS m/z 565 [M+ + H].


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  		TABLE 4. 1H NMR data in CDCl3

Inhibitory activity against lipid peroxidation.
In vitro inhibitory activity of the carotenoids against lipid peroxidation was measured by the oxygen radical-generating reaction, which was initiated with sodium ascorbate in a rat brain homogenate, as described previously (48).

Nucleotide sequence accession number.
The nucleotide sequence of the 12.0-kb EcoRI region of p5Bre2-15 has been deposited in DDBJ/EMBL/GenBank under accession number AB181388.


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RESULTS
 
Isolation of a DNA fragment including crtI.
The crtI gene encoding phytoene desaturase has been isolated and characterized for a variety of organisms. A comparison of the deduced amino acid sequences of CrtI proteins from Paracoccus sp. strain N81106 (MBIC01143) (31), Paracoccus zeaxanthinifaciens ATCC 21588 (formerly classified as Flavobacterium sp. strain R1534) (6, 37), Pantoea agglomerans (formerly classified as Erwinia herbicola strain Eho10) (4), Bradyrhizobium sp. strain ORS278 (17), and Erythrobacter longus strain Och101 (25) has revealed well-conserved domains in the N-terminal region (FDAGPTV) and C-terminal region (GAGIPG). Genomic DNA was isolated from the cells of Brevundimonas sp. strain SD212 (MBIC03018), whose 16S rRNA gene and gyrB sequences are available in DDBJ/EMBL/GenBank under accession numbers AB016849 and AB014993, respectively. Based on the degenerate primers designed from the above-mentioned motifs, PCR amplification was done to isolate a 1.1-kb fragment from the SD212 genomic DNA and ligated into pGEM-T Easy to yield plasmid pCRTI-SD212 (Table 1). The nucleotide sequence of this 1.1-kb inserted fragment was similar to the known CrtI sequences at the amino acid level.

A cosmid library of the genomic DNA from Brevundimonas sp. strain SD212, which comprised 1,000 colonies, was constructed using cosmid vector SuperCos 1 (Table 1). Colony hybridization experiments on 500 of these colonies were conducted with the DNA probe of the 1.1-kb fragment from pCRTI-SD212. As a result, six colonies showing positive signals were obtained. Cosmid DNA was prepared from these colonies and subjected to Southern hybridization experiments after being digested with appropriate restriction enzymes; common fragments, i.e., 12-kb EcoRI, 9-kb BamHI, and 8.2 kb-EcoRI/BamHI, showed positive hybridization signals. These signals were also detected in the digested fragments that had been derived from the SD212 genomic DNA. One cosmid (named pCos5-2) was used for further experiments. A 12-kb EcoRI fragment was isolated from pCos5-2 and inserted into the EcoRI site of vector pBluescript II KS(–) to yield plasmid p5Bre2-15 (Table 1).

Structural analysis of the carotenoid biosynthesis gene cluster.
The nucleotide sequence of the 12-kb EcoRI fragment of plasmid p5Bre2-15 revealed this fragment, which was 11,991 bp in length and had a high GC content (69%), to contain 12 ORFs. Seven ORFs were similar to the known carotenogenic genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ, and the same designations were assigned (Fig. 1a and Table 2). The translated sequences of the other five ORFs showed no overall homology with any other proteins. The crtY, crtI, and crtB genes occurred in this order and were oriented in the same direction, and these characteristics have been observed in all of the cyclic (dicyclic) carotenoid biosynthesis gene clusters previously reported (Fig. 1b). The organization of the SD212 carotenoid biosynthesis gene cluster did, however, have several unique characteristics. (i) The sequence for type 2 isopentenyl diphosphate (IDP or IPP) isomerase (idi) (22) was present in this cluster. Interestingly, this idi sequence has only previously been found in the carotenoid biosynthesis gene cluster from P. agglomerans (E. herbicola Eho10), the gene of which exists between the crtE and crtX genes (19), and had remained unidentified for a decade. (ii) The crtW and crtZ genes, which mediate the oxygenation reaction from ß-carotene to astaxanthin and exist adjacently in the Paracoccus strains, were present at a distance from each other in Brevundimonas sp. strain SD212. (iii) Five transcription units are likely to be present in this gene cluster for the expression of all seven crt genes and five ORFs. (iv) The individual SD212 carotenogenic gene products showed low or medium levels of amino acid homology to the corresponding proteins derived from the other genera of bacteria reported (31 to 72%) (Fig. 1b). (v) No substantial difference has been observed in the degree of amino acid homology with the Brevundimonas Crt proteins between those derived from the Pantoea species of {gamma}-Proteobacteria and the other bacterial species of {alpha}-Proteobacteria (Fig. 1b).



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  		FIG. 1. Organization of the carotenoid biosynthesis gene clusters isolated from Brevundimonas sp. strain SD212 and from other cyclic carotenoid-generating bacteria. (a) Organization of the Brevundimonas sp. strain SD212 carotenoid biosynthesis gene cluster composed of an 11,991-bp EcoRI fragment. Numbers represent nucleotide positions numbered from the 5' end of the EcoRI site. The numbers over and under the gene map, respectively, represent the positions of the start and termination of individual genes or ORFs. ORF11 was identified as a 2,2'-ß-hydroxylase gene in this study and is designated crtG. (b) Comparison of the organization of the cyclic carotenoid gene clusters of Paracoccus sp. strain N81106 (GenBank accession number D58420) (31), Paracoccus zeaxanthinifaciens (U62808) (37), Xanthobacter autotrophicus Py2 (AF408848) (23), Bradyrhizobium sp. strain ORS278 (AF218415) (17), Pantoea ananatis (formerly classified as Erwinia uredovora 20D3, D90087) (30), and Pantoea agglomerans (M87280) (19). Arrows represent the orientations of genes. The values shown below the genes indicate the percentages of amino acid identity compared with Brevundimonas sp. strain SD212, which were calculated by using Clustal W (18) and GeneDoc (35). The zeaxanthin-hyperproducing bacterium that was formerly classified as Flavobacterium sp. strain R1534 has recently been reported to be the mutant of P. zeaxanthinifaciens ATCC 21588, as taxonomically reevaluated by Berry et al. (6). Note that all of the other bacteria, except for the genus Pantoea of the {gamma}-Proteobacteria, belong to the {alpha}-Proteobacteria.


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  		TABLE 2. Gene products of individual ORFs in the 12-kb fragment of Brevundimonas sp. strain SD212 and homology with other proteins

Functional analysis of individual carotenoid biosynthesis genes.
Functional analysis of many carotenoid biosynthesis genes has successfully been carried out in E. coli. However, both E. coli transformants carrying the entire crt gene cluster of Brevundimonas sp. strain SD212 (plasmid p5Bre2-15) and carrying plasmid pACCRT-EB for phytoene production in addition to p5Bre2-15 did not produce any pigmented carotenoids. Moreover, p5Bre2-15 was not able to complement E. coli carrying pACCAR25{Delta}crtE, pACCAR25{Delta}crtB, pACCRT-EIB, pACCAR16{Delta}crtX, or pACCAR25{Delta}crtX to produce the additional carotenoid pigments, suggesting that these genes are not easily expressed in E. coli. It is possible that translational signals such as the ribosomal binding sites that are included in the gene cluster are not functional in E. coli.

Thus, to analyze the functions of the individual crt genes or ORFs of SD212, we devised a plasmid construct for their expression in E. coli which would be able to synthesize each gene product fused to a lead sequence of ß-galactosidase (LacZ) under transcription from the lac promoter by vector pUC18. The lead sequence in these plasmids, with the seven amino acid residues Met-Thr-Met-Ile-Thr-Asn-Ser derived from LacZ, was designed to be added to the Met of the original start of the individual genes. These hybrid genes are expected to be efficiently translated using the original ribosomal binding site and start codon of the lacZ gene. These plasmids were pUCBre-E, pUCBre-B, pUCBre-I, pUCBre-Y, pUCBre-Z, and pUCBre-W for the expression of the individual SD212 crt genes (Table 3) and pUCBre-O1, pUCBre-O6, pUCBre-O7, pUC-Bre-O11, and pUCBre-O12 for the respective expression of the unknown SD212 ORFs, ORF1, ORF6, ORF7, ORF11, and ORF12. These plasmids (Apr) were introduced into E. coli transformants, accumulating the desired carotenoid precursors due to the presence of the pACYC184-derived plasmids with Cmr that carried the various combinations of bacterial crt genes for complementation studies (Table 3). The identification of pigments produced in the resulting E. coli transformants with Cmr and Apr exhibited the individual crt genes of strain SD212 coding for proteins with the predicted catalytic functions (Table 3).


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  		TABLE 3. Analysis of carotenoids accumulated in E. coli transformants carrying various combinations of crt genes from Brevundimonas sp. strain SD212 and other bacterial strains

Since Brevundimonas sp. strain SD212 produced the ketocarotenoids with the 2 (2')-hydroxy group, it was possible to include a novel oxygenase gene encoding 2,2'-ß-hydroxylase among ORF1, ORF6, ORF7, ORF11, and ORF12. Therefore, the plasmids for expressing these ORFs, i.e., pUCBre-O1, pUCBre-O6, pUCBre-O7, pUCBre-O11, and pUCBre-O12, were introduced into E. coli, accumulating astaxanthin and the intermediates such as adonixanthin due to the presence of plasmid pAC-Asta, with these accumulated pigments analyzed by HPLC. It was found that only E. coli carrying pUCBre-O11 and pAC-Asta could produce an additional polar carotenoid besides the carotenoids accumulated in a control E. coli strain carrying only one plasmid, pAC-Asta. The HPLC profile is shown in Fig. 2a. Major carotenoid pigments 1, 2, and 3 in the extract from the cells were purified, and various instrumental analyses were conducted to determine the structures. Pigments 2 and 3, which also appeared in the control strain, were confirmed to be astaxanthin and adonixanthin by their LC/APCI-MS and UV-VIS spectral data. Pigment 1 was identified as 2-hydroxyastaxanthin by its LC/APCI-MS, UV-VIS, and 1H NMR spectral data (55). The stereostructure of the 2-hydroxy group was assigned to be R according to the circular dichroism spectral data of the carotenoids identified in strain SD212 (55). It is therefore clear that ORF11 coded for the novel carotenoid biosynthesis enzyme, carotenoid 2,2'-ß-hydroxylase (ß-ring C2-hydroxylase). This gene was designated crtG. crtG nomenclature was used only once by Schnurr et al. in 1991 (44), instead of the crtX gene coding for zeaxanthin glucosyltransferase (30), and has never been used since then, since crtX has been in common use.



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  		FIG. 2. Carotenoid products from E. coli carrying pUCBre-O11 and pAC-Asta (a), pUCBre-O11 and pACCAR25{Delta}crtX (b), and pUCBre-O11 and pAC-Cantha (c). HPLC/PDA was performed using a TSK gel ODS-80Ts column, as described in Materials and Methods. Detection was carried out at 470 nm. The numbers of carotenoids correspond to those discussed in "Spectral data for individual carotenoids" in Materials and Methods.

The carotenoid pigments that Brevundimonas sp. strain SD212 produced had already been identified (55), and their structures are shown in Fig. 3. Since we seem to have identified the functions of all of the SD212 carotenoid biosynthesis gene products, the carotenoid biosynthetic pathway was proposed at the gene level as shown in Fig. 3. Except for (2R)-2-hydroxyastaxanthin, we could not detect the ketocarotenoids with a 2 (2')-hydroxyl group in the extract from E. coli cells expressing crtG as well as the genes required for astaxanthin synthesis. However, it should be feasible to detect these ketocarotenoids by enhancing the expression level of the carotenoid biosynthesis-related genes in a heterologous host such as E. coli, as discussed in Discussion.



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  		FIG. 3. Chemical structures of the carotenoid pigments produced by Brevundimonas sp. strain SD212 (55) and proposed functions of the carotenoid biosynthesis gene products. The functions of CrtE, CrtB, CrtI, and CrtY are shown in Fig. 4. FPP, farnesyl diphosphate (pyrophosphate).

Combinatorial biosynthesis of new and rare carotenoids using the new 2,2'-ß-hydroxylase gene.
The acquisition of a new class of carotenoid biosynthesis gene should make it possible to achieve combinatorial biosynthesis of various new or rare carotenoids by using the gene. Thus, plasmid pUCBre-O11 was introduced into E. coli carrying pACCAR25{Delta}crtX that accumulates zeaxanthin, and the yellow cells of the resulting E. coli transformant were subjected to pigment analysis. The HPLC profile is shown in Fig. 2b. Pigments 4, 5, and 6 produced by this E. coli transformant (pUCBre-O11 and pACCAR25{Delta}crtX) were predicted to be carotenoids involving the 3-hydroxy-ß-ring as the end groups corresponding to those of zeaxanthin. Pigment 6 was found to be zeaxanthin from its LC/APCI-MS analysis and by comparison with an authentic sample. Pigment 4, which displayed m/z 601 [M+ + H], 583 [M+ + H – H2O], and 565 [M+ + H – 2H2O], and pigment 5, which displayed m/z 585 [M+ + H] and 567 [M+ + H – H2O] by LC/APCI-MS analysis, were identical to 2, 2'-dihydroxyzeaxanthin and 2'-hydroxyzeaxanthin, respectively. Their 1H NMR data are shown in Table 4. The coupling constant between H-2 (H-2') and H-3 (H-3') of 10 Hz predicted that the H-2 (H-2') proton and the 2 (2')-hydroxyl group would have axial and equatorial confirmations, respectively. Since zeaxanthin synthesized with an E. coli transformant carrying the crtE, crtB, crtI, crtY, and crtZ genes of P. ananatis (Erwinia uredovora 20D3) has shown the 3R,3'R configuration (15), the stereostructure of C-2,2' in pigment 4 was determined to be R. Pigment 5 was found to have the same 2,3-dihydroxy-ß ring as that of pigment 4 and the same 3-hydroxy-ß-ring as that of zeaxanthin from its 1H NMR data (Table 4). These results enabled pigments 4 and 5 to be identified as (2R, 2'R)-dihydroxyzeaxanthin (nostoxanthin) and (2R)-hydroxyzeaxanthin (caloxanthin), respectively, which are rarely found in nature. The biosynthetic pathway to caloxanthin and nostoxanthin by the recombinant E. coli is shown in Fig. 4.



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  		FIG. 4. Combinatorial biosynthesis of novel and rare carotenoids by using E. coli carrying plasmids pUCBre-O11 and pACCAR25{Delta}crtX or pAC-Cantha.

Furthermore, plasmid pUCBre-O11 was introduced into E. coli carrying pAC-Cantha, which accumulates canthaxanthin, and the orange cells of the resultant E. coli transformant were subjected to pigment analysis. The HPLC profile is shown in Fig. 2c. Pigments 7, 8, and 9 produced by this E. coli transformant (pUCBre-O11 and pAC-Cantha) were carotenoids showing a 1H NMR signal of 4-keto-ß type. Pigment 9 was found to be canthaxanthin from its LC/APCI-MS analysis and by comparison with an authentic sample. Pigment 7 was predicted to have a symmetrical structure from its 1H NMR data (Table 4), and its COSY, HSQC, and HMBC spectral data showed that pigment 7 had hydroxyl groups at the 2 and 2' positions. The correlation of H-16 (16') and H-17 (17') with C-2 (2') of ca. 75 ppm supported the 2,2'-dihydroxyl groups. Taking the results of HRFABMS and LC/APCI-MS into consideration, pigment 7 was identified as the novel carotenoid 2,2'-dihydroxycanthaxanthin (2, 2'-dihydroxy-ß,ß-carotene-4,4'-dione). Since the respective coupling constants between H-2 and H-3ax (H-2' and H-3ax) and H-2 and H-3eq (H-2' and H-3eq) were 9 Hz and 5 Hz, the H-2 (2') proton and the 2 (2')-hydroxyl group had axial and equatorial conformations, respectively.

Pigment 8 was found to have the 2-hydroxy-4-keto-ß-ring like pigment 7 and the 4-keto-ß-ring like canthaxanthin from its 1H NMR data (Table 4). Thus, pigment 8 was identified as 2-hydroxycanthaxanthin [2-hydroxy-ß,ß-carotene-4,4'-dione]from its HRFABMS, LC/APCI-MS, and 1H NMR data. This pigment has only previously been isolated from a marine animal (Crustacea) (11). The R chirality at C-2 of pigment 8 was confirmed by the coincidence with the spectral data of (2R)-2-hydroxycanthaxanthin described in this paper. Therefore, the stereostructure of C-2, 2' in novel pigment 7 was determined to be of R configuration. The biosynthetic pathway to (2R)-2-hydroxy-ß,ß-carotene-4,4'-dione and (2R, 2'R)-2,2'-dihydroxy-ß,ß-carotene-4,4'-dione by the recombinant E. coli is shown in Fig. 4.

When the crtG gene (plasmid pUCBre-O11) was introduced into ß-carotene-synthesizing E. coli carrying plasmid pACCAR16{Delta}crtX, the resulting E. coli transformant was not able to produce detectable 2-hydroxy carotenoids. Therefore, CrtG is likely to require an oxygenated ß-end group, i.e., a 3-hydroxy- or 4-keto-ß-end group as a substrate for the 2-hydroxylation reaction.

Antioxidative activity of new and rare ketocarotenoids.
We examined the in vitro inhibitory effects of several carotenoids, including the new xanthophyll, 2,2'-dihydroxycanthaxanthin, toward the lipid peroxidation induced by free radicals in a rat brain homogenate, as shown in Table 5. It was consequently suggested that the 2-hydroxy and 2,2'-dihydroxy products had much stronger antioxidative activity than the precursor canthaxanthin.


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  		TABLE 5. Inhibitory effects of several carotenoids on lipid peroxidation in a rat brain homogenate


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DISCUSSION
 
The present study has enabled us to elucidate for the first time the structures and functions of a gene cluster encoding carotenoid biosynthetic enzymes from the genus Brevundimonas belonging to {alpha}-Proteobacteria. This cluster contained the seven known genes crtW, crtY, crtI, crtB, crtE, idi, and crtZ in addition to a new gene (designated crtG), which was found to encode a novel enzyme, carotenoid 2,2'-ß-hydroxylase. This enzyme, CrtG, composed of 257 amino acid residues showed no overall homology with any other proteins. However, this enzyme surprisingly exhibited intriguingly partial homology with the middle region of animal sterol-C5-desaturase ({Delta}7-sterol 5-desaturase), which catalyzes the oxidation reaction from lathosterol to 7-dehydrocholesterol in the pathway for cholesterol biosynthesis (Fig. 5). The degree of identity of CrtG (amino acids 51 to 233) with the middle region of sterol-C5-desaturases was 28%. It is difficult to speculate on the function of the homologous sequences between the two enzymes, but they may be involved in their binding to the isoprenoid substrates and/or in the common oxidation reactions.



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  		FIG. 5. Alignment of the deduced amino acid sequences of CrtG from Brevundimonas sp. strain SD212 and sterol-C5-desaturases (C5D) derived from human (Homo sapiens) (GenBank accession no. AB016247), mouse (Mus musculus) (GenBank accession no. BC024132), and rat (Rattus norvegicus) (GenBank accession no. AB052846) origins. Asterisks and dots indicate amino acid residues identical and similar, respectively, for all of the aligned sequences. Identical or similar residues among all aligned sequences are shown with a black background. A gray background indicates partially aligned sequences. Arrows indicate the homologous region (the amino acids shown for CrtG are 51 to 233).

This study has also demonstrated that we can produce a variety of structurally novel or rare carotenoids with a 2-hydroxy group and 2,2'-dihydroxy groups by using crtG in various combinations with other known crt genes. This successful combinatorial biosynthesis of such xanthophylls with a new functional group(s), which are very difficult to synthesize chemically, seems to make it feasible to evaluate their pharmaceutical potential, as we have here suggested their potential as superior antioxidants. It should be feasible to enhance the productivity of these carotenoids by using E. coli that has an improved isoprenoid central pathway due to the introduction of such key biosynthesis genes as DXP (1-deoxy-D-xylose 5-phosphate) synthase and IDP (IPP) isomerase genes (1, 10, 26, 52) or by using other efficient heterologous hosts such as the yeast Candida utilis (47) and higher plants (14, 46). Directed evolution, such as DNA shuffling (43, 50), may also be applicable to enhancing or changing the substrate affinity or preference of the CrtG enzyme for the 2 (2')-hydroxylation reaction; this technique may make it possible to produce a CrtG enzyme which can catalyze the efficient conversion from ß-carotene to 2,2'-dihydroxy-ß-carotene by way of 2-hydroxy-ß-carotene.


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ACKNOWLEDGMENTS
 
We thank Akihiro Hara, Satoru Matsuda, Yukie Inomata, Michiru Suzuki (Marine Biotechnology Institute), and Atsuko Suzuki (Japan Women's University) for technical contributions.

This work was supported by the Biotechnology and Medical Technology Development Department of the New Energy and Industrial Technology Development Organization (NEDO).


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FOOTNOTES
 
* Corresponding author. Mailing address: Marine Biotechnology Institute, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan. Phone: 81 193 26 6581. Fax: 81 193 26 6584. E-mail: norihiko.misawa{at}mbio.jp. Back


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Applied and Environmental Microbiology, August 2005, p. 4286-4296, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4286-4296.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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