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Expression of Nitrite and Nitric Oxide Reductases in Free-Living and Plant-Associated Agrobacterium tumefaciens C58 Cells
Appl. Environ. Microbiol. Baek and Shapleigh 71: 4427

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Alignment of primary sequences of copper-containing nitrite reductases from various denitrifiers (Supplemental Figure 1); alignment of primary sequences of two subunits of nitric oxide reductases from various denitrifiers (Supplemental Figure 2); micrographs of Arabidopsis root hairs prior to and at 10 h after exposure to A. tumefaciens, demonstrating that seedlings contained attached, nonfluorescent bacteria (Supplemental Figure 3); microscopic examination of a root hair from a seedling after incubation with two strains of A. tumefaciens to demonstrate that both wild and Nir-deficient strains bound to root seedlings (Supplemental Figure 4); micrographs of Arabidopsis root tips incubated with two strains of A. tumefaciens to demonstrate that addition of nitrate enhanced expression of GFP in wild-type bacteria associated with roots but not in Nir-deficient strains (Supplemental Figure 5).
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