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Applied and Environmental Microbiology, June 2007, p. 3575-3580, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00011-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of SafC, a Catechol 4-O-Methyltransferase Involved in Saframycin Biosynthesis

James T. Nelson,^1, Jaeheon Lee,^2, James W. Sims,¹ and Eric W. Schmidt^1,2*

Department of Medicinal Chemistry,¹ Department of Biology, University of Utah, Salt Lake City, Utah 84112²

Received 3 January 2007/ Accepted 10 April 2007

	ABSTRACT

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Members of the saframycin/safracin/ecteinascidin family of peptide natural products are potent antitumor agents currently under clinical development. Saframycin MX1, from Myxococcus xanthus, is synthesized by a nonribosomal peptide synthetase, SafAB, and an O-methyltransferase, SafC, although other proteins are likely involved in the pathway. SafC was overexpressed in Escherichia coli, purified to homogeneity, and assayed for its ability to methylate a variety of substrates. SafC was able to catalyze the O-methylation of catechol derivatives but not phenols. Among the substrates tested, the best substrate for SafC was L-dihydroxyphenylalanine (L-dopa), which was methylated specifically in the 4'-O position (k_cat/K_m = 5.5 x 10³ M^–1 s^–1). SafC displayed less activity on other catechol derivatives, including catechol, dopamine, and caffeic acid. The more labile L-5'-methyldopa was an extremely poor substrate for SafC (k_cat/K_m = 2.8 x 10^–5 M^–1 s^–1). L-Dopa thioester derivatives were also much less reactive than L-dopa. These results indicate that SafC-catalyzed 4'-O-methylation of L-dopa occurs prior to 5'-C-methylation, suggesting that 4'-O-methylation is likely the first committed step in the biosynthesis of saframycin MX1. SafC has biotechnological potential as a methyltransferase with unique regioselectivity.

	INTRODUCTION

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Isoquinoline-containing natural products are widely used in biology and medicine because of their potent activities in a large number of biological assays (4, 7, 13, 21). Saframycins represent a class of isoquinoline alkaloids exhibiting antibiotic and antitumor activities (1). Since the original report of these molecules, a large number of analogs have been isolated, including saframycins and safracins from bacteria, renieramycins from sponges (phylum Porifera), and ecteinascidins from ascidians (phylum Chordata) (16, 19, 20, 29). The compounds interact with DNA, forming covalent bonds that are thought to be important in their cytotoxicity (37). Other mechanisms of action, either dependent upon or independent of DNA binding, have been reported (11). Interest in these molecules has been fueled by the advancement of ecteinascidin-743 to phase II/III clinical trials (12). Robust synthetic and semisynthetic approaches have been described to obtain ecteinascidin-743, which otherwise must be harvested by aquaculture or from wild stocks of the ascidian (9, 10). In order to improve the ability to synthesize analogs, a genetic approach has also been taken to modify the safracin biosynthetic pathway (33). Additional understanding of saframycin biosynthetic machinery and mechanisms would greatly aid in developing mutational approaches for the production of novel isoquinolines.

A number of precursor feeding studies with the saframycin family of metabolites have shown that the core structure is composed of amino acids (Fig. 1) (2). For example, safracins are composed of Ala, Gly, and two Tyr subunits, which are further elaborated by oxidative steps and by methylation with S-adenosylmethionine (33). Other biosynthetic steps, such as the folding of the tetrapeptide precursor into the final compound, remain obscure. Alternative possible routes to heterocycle formation include either Pictet-Spengler (P-S) or Bischler-Napieralski condensation (14).

View larger version (7K): [in this window] [in a new window]   FIG. 1. Proposed biosynthetic route to saframycin MX1. (i) SAM methylation. (ii) NRPS-catalyzed formation of a tetrapeptide intermediate. This intermediate is folded by a Pictet-Spengler or Bischler-Napieralski condensation into a saframycin precursor. Further methylation and oxidation lead to the formation of saframycin MX1. This study defines intermediate 4'-O-methyl-L-dopa as the first committed intermediate; previously, the timing of C- versus O-methylation was unclear.

SafC is homologous to a variety of O-methyltransferases, particularly those involved in the methylation of catechols. Catechol O-methyltransferases (COMTs) are widely important in biology, agriculture, and medicine. For example, they are involved in the degradation of dopamine and estrogen as well as in the synthesis of caffeic acid and important plant natural products (5, 6, 15, 38). The characterized COMTs are primarily 3'-O-methyltransferases, while some produce a mixture of both 3'- and 4'-O-methylated products (24). Such 4'-O-methylation seems to play a role in the metabolism of anthocyanins and catechins (18, 23). There are two examples of methyltransferases selective for the para-hydroxyl group in benzylisoquinoline alkaloids in plants, although this methylation takes place after the Pictet-Spengler folding step (25, 39). Because L-dihydroxyphenylalanine (L-dopa) is O-methylated at the 4' position in saframycins, SafC was potentially a new catalyst that could find use in the study of catechol metabolism and natural product biosynthesis. As part of our biochemical analysis of the saf pathway, we report the kinetic characterization of the SafC O-methyltransferase.

	MATERIALS AND METHODS

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General.
Chemicals were purchased from Sigma-Aldrich, except for derivatives of L-dopa, which were synthesized as described below or in a previous work (32). For all catechol derivatives with the exception of thioesters, authentic unmethylated, 3-O-methylated, and 4-O-methylated standards were available and used for regioselectivity studies. S-[methyl-³H]Adenosyl-L-methionine was purchased from PerkinElmer. High-pressure liquid chromatography (HPLC) was performed using a Hitachi L6200 intelligent pump and L3000 photo diode array detector with either a 100-Å Microsorb MV C₁₈ column (Varian) or a Phenomenex Gemini 5-µm C₁₈ column (250 by 4.6 mm). Thin-layer chromatography (TLC) analysis was performed using aluminum-backed silica plates with 13:5:2 butanol-water-acetic acid as the mobile phase and ninhydrin-based detection. M. xanthus Mx x48 was provided by H. Reichenbach.

N-(tert-Butyloxycarbonyl)-3,4-dihydroxy-L-phenyalanine thiophenolate.
L-Dopa (300 mg, 1.5 mmol) was added to aqueous NaOH (1 M, 1.5 ml) in 1,4-dioxane (3 ml). The resulting solution was stirred for 10 min at 4°C, and then di-tert-butyldicarbonate (330 mg, 1.5 mmol) was added. The reaction was stirred overnight at 25°C. A rotary evaporator was used to remove dioxane, and the remaining solution was acidified with 1 ml of 1 N HCl and extracted with ethyl acetate. The crude product was dried under Ar and then dissolved in N,N-dimethylformamide (200 µl). A solution of (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (210 mg, 0.40 mmol) and benzenethiol (50 µl, 0.41 mmol) in N,N-dimethylformamide (200 µl) was then added, and the resulting mixture was stirred for 1 h at 25°C. K₂CO₃ (27.6 mg, 0.20 mmol) was added, and the reaction was stirred for 2 h at 25°C. Phosphate buffer (1 ml, 10 mM, pH 6) was added, and the mixture was extracted with ethyl acetate. Following column chromatography (silica; 30 to 50% ethyl acetate in hexane gradient), Boc-L-dopa thiophenolate (35.0 mg, 90 µmol, 6% yield over two steps) was obtained. ¹H nuclear magnetic resonance (NMR), (CDCl₃, 400 MHz) 7.3 to 7.4 (5H, m), 6.78 (1H, d, J = 8 Hz), 6.67 (1H, d, J = 2 Hz), 6.57 (1H, dd, J = 2, 8 Hz), 5.12 Hz (1H, d, J = 8.8 Hz), 4.66 (1H, m), 3.00 (2H, m), 1.41 (9H, s); ¹³C NMR (CDCl₃, 125 MHz) 200.1, 155.5, 144.1, 143.2, 134.6, 129.5, 129.2, 127.7, 127.0, 121.6, 116.2, 115.4, 81.0, 60.6, 37.8, 28.3; high-resolution fast atom bombardment mass spectrum, m/z = 390.1372 [M+H]⁺, C₂₀H₂₄NO₅S requires m/z = 390.1375 ( = –0.3 ppm).

Synthesis of L-dopa-CoA.
Boc-L-dopa-thiophenolate (4.8 mg, 0.01 mmol) was suspended in 1:1 dichloromethane-trifluoroacetic acid (0.2 ml) and stirred for 30 min at ambient temperature. Following the removal of solvent under a stream of Ar, the crude product was dissolved in a solution of coenzyme A (CoA) sodium salt (8.2 mg, 0.01 mmol) in aqueous phosphate buffer (200 µl, 10 mM, pH 8). The resulting mixture was stirred for 2.5 h at 25°C and then injected onto a Phenomenex C₁₈ analytical HPLC column with a 1-ml/min flow rate. The mobile phase consisted of 10 mM aqueous ammonium acetate and acetonitrile. The major compound at 280 nm was collected, lyophilized to yield L-dopa-CoA as an acetate salt (3.9 mg, 0.004 mmol, 35%), and confirmed by ¹H NMR and mass spectrometry analyses: ¹H NMR, (CD₃OD, 400 MHz) 8.56 (1H, s), 8.18 (1H, s), 6.75 (1H, s), 6.67 (1H, d, J = 8.4 Hz), 6.53 (1H, d, J = 7 Hz), 6.11 (1H, d, J = 6.4 Hz), 4.79 (1H, m), 4.47 (1H, br), 4.27 (2H, br), 4.18 (1H, m), 4.08 (1H, s), 3.99 (1H, dd, J = 5.2, 8.4 Hz), 3.60 (1H, m), 3.54 (1H, m), 3.39 (1H, s), 3.25 (1H, m), 3.14 (1H, m), 2.97 (3H, m), 2.41 (2H, m), 1.91 (3H, s), 1.28 (1H, t, J = 7.2 Hz), 1.17 (1H, t, J = 7.2 Hz), 1.06 (3H, s), 0.84 (3H, s); matrix-assisted laser desorption ionization-time of flight mass spectrum, m/z = 947.36 [M+H]⁺, C₃₀H₄₆N₈O₁₉P₃S requires m/z = 947.17.

Bacterial strains, plasmids, and culture conditions.
Escherichia coli strain DH5 was used for routine subcloning and was transformed as described by Sambrook and Russell (31). For protein expression, E. coli BL21(DE3) competent cells were used with standard procedures (Novagen). To construct the vector harboring the SafC gene, PCR was performed with Pfu DNA polymerase (Stratagene), using primers SafCF (5'-GGAATTCCATATGATCCACCACGTCGAATTGACACAG-3', NdeI site underlined) and SafCR (5'-GGGAAGCTTGCGCTTGCGAGCGAGGGT-3', HindIII site underlined). Amplification was achieved with 30 cycles of denaturation at 94°C for 30 s, annealing at 53°C for 30 s, and extension at 72°C for 5 min. The 663-bp PCR product was recovered by 1% agarose gel electrophoresis. After digestion with NdeI and HindIII, the fragment was inserted into pET20b to generate pSafC with a C-terminal hexahistidine tag.

Protein expression and purification.
E. coli BL21(DE3) harboring pSafC was grown at 37°C in a shake flask in LB medium in the presence of 50 µg/ml of ampicillin. Overnight cultures were diluted to 1:1,000 in fresh medium and grown to an optical density at 600 nm of 0.6 until the addition of isopropyl-ß-D-thiogalactopyranoside (IPTG) at a final concentration of 1 mM. Induction was continued for 2 h, and the cells were harvested.

The cell pellet was resuspended in 10 ml lysis buffer (50 mM Tris-HCl, 500 mM NaCl, and 10 mM imidazole, pH 8.0). Cells were disrupted by sonication, and the lysate was clarified by centrifugation (45 min at 14,400 x g). The protein was purified on Ni-nitrilotriacetic acid resin (QIAGEN). SafC was eluted with a linear gradient of imidazole from 20 to 250 mM and then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fractions containing pure protein were dialyzed against buffer (50 mM Tris-HCl, 0.2 mM MgCl₂, 2 mM dithiothreitol [DTT], and 10% glycerol, pH 7.5). After overnight dialysis, the samples were quantified by the Bradford protein dye assay, aliquoted, and stored frozen at –80°C until use.

Large-scale SafC reaction monitored by ¹H NMR.
L-Dopa-CoA was used in a large-scale reaction with SafC in D₂O in an NMR tube. SafC (7.5 µg) was added to 10 mM (pH 8.0) phosphate buffer (450 µl) with S-adenosylmethionine (SAM; 1 mg) and L-dopa-CoA (1 mg). The reaction was monitored by ¹H NMR (D₂O, 400 MHz).

HPLC characterization of SafC regioselectivity.
SafC (1.6 µM) and either L-dopa (500 µM) or L-dopa-CoA (200 µM) were added to HEPES (10 mM, pH 7.2), MgCl₂ (200 µM), DTT (2 mM), and SAM (200 µM) in 50-µl reaction volumes. Enzyme reaction mixtures were incubated overnight at 37°C. Reaction quenching and thioester hydrolysis were accomplished by adding 5.5 µl of 1 M Na₂HPO₄ and boiling the reaction mix for 15 min. The reaction mixtures were then frozen at –80°C until analyzed by HPLC. Appropriate standards and the products of SafC-L-dopa-CoA and SafC-L-dopa reactions were resolved using a 100-Å Microsorb MV C₁₈ column (Varian) in an aqueous solvent consisting of 80.3 ml of methanol, 75 ml of 1 M NaH₂PO₄, 22.5 ml of 1 M EDTA, and 695.3 ml of H₂O. Additional L-dopa reactions and standards were resolved using a Phenomenex Gemini 5-µm C₁₈ column (250 by 4.6 mm) in an aqueous buffer: 960 ml of 40 mM MgCl₂, pH 3.0, and 40 ml of acetonitrile.

L-Tyr reactivity was assessed in overnight reactions as with L-dopa, except that reactions were not quenched prior to HPLC analysis. Similar reactions of SafC with dopamine and caffeic acid were also performed, and the products were resolved and analyzed by HPLC: caffeic acid, isocratic in 125 ml of acetonitrile and 375 ml of 10.42 mM MgCl₂, pH 3.0; dopamine, gradient run in acetonitrile and 10.42 mM MgCl₂, pH 3.0.

SafC kinetics.
4'-O-Methyl-L-dopa, 5'-methyl-L-dopa, and 4'-O-methyl-5'-methyl-L-dopa were previously synthesized (32). SafC (0.78 µM) and various concentrations of substrate were incubated with HEPES (10 mM, pH 7.2), MgCl₂ (100 µM), DTT (2 mM), and SAM (400 µM) at 37°C. The optimum buffer composition and pH were determined by various parameters to achieve maximum reaction rates with L-dopa. Optimum SAM concentrations were determined by varying the amount of SAM under conditions of saturating L-dopa (507 µM) to measure an approximate V_max and K_m. Since the V_max was achieved at a SAM concentration of 300 µM, a saturating amount of SAM was used in all reactions. Following incubation, reaction vials were frozen in liquid nitrogen. Reaction products were resolved using a Phenomenex Gemini 5-µm C₁₈ column (250 by 4.6 mm). D-6000 HPLC Manager software (Hitachi) was used for the integration of product peaks, and KaleidaGraph 4.0 (Synergy Software) was used to graph and fit data by using the Michaelis-Menten approximation.

Triplicate L-dopa reactions were run for 20 min at concentrations of 25 µM, 50 µM, 75 µM, 190 µM, 300 µM, 370 µM, and 480 µM. HPLC product resolution of L-dopa reactions was accomplished in a pH 3 aqueous solvent (960 ml of 40 mM MgCl₂, pH 3.0, and 40 ml of acetonitrile). Reactions with catechol were run similarly; however, triplicate reactions were each run for a period of 50 min at substrate concentrations of 36 µM, 270 µM, 900 µM, 2,200 µM, and 3,100 µM. Product resolution of catechol reactions was performed in an aqueous buffer consisting of 125 ml of acetonitrile and 375 ml of 40 mM MgCl₂ at pH 3.0.

Approximate kinetics for SafC reacting with 5'-methyl-L-dopa were obtained using single, 60-min reactions at substrate concentrations of 1 mM, 2 mM, 5 mM, 10 mM, 18 mM, and 24 mM. The HPLC solvent for resolving these later reaction products was 187 ml of 40 mM MgCl₂ at pH 3 combined with 17 ml of acetonitrile. Standard curves for reactions with all three substrates were produced using 4'-O-methyl-L-dopa, 4'-O-methyl-5'-methyl-L-dopa, and guaiacol.

For caffeic acid and dopamine, similar reactions were run under various substrate concentrations of 25 µM, 50 µM, 75 µM, 190 µM, 300 µM, 370 µM, 480 µM, and 800 µM. Reactions were analyzed by HPLC in 125 ml of acetonitrile and 375 ml of 10.42 mM MgCl₂, pH 3.0. The dopamine reactions were similarly analyzed but with an acetonitrile gradient.

L-Dopa-CoA reactivity.
Kinetic runs were performed using L-dopa-CoA, following the method used for L-dopa, except that reactions were quenched by boiling in various bases prior to HPLC analysis. Additionally, a series of runs was performed in which L-dopa-CoA hydrolysis to L-dopa and CoA was measured using Ellman's reagent at 25°C. A 1-ml solution containing 100 µM MgCl₂, 10 mM HEPES, pH 7.2, 30 µl of ethanol, and 2 mM Ellman's reagent was used to zero a UV spectrophotometer. To this solution was added 10 µl of dopa-CoA (1 mg ml^–1). Absorbance at 412 nm was monitored every 1 min for 30 min and then after 60 min and 120 min. A standard curve was prepared using the same buffer containing free CoA.

L-Dopa-CoA kinetics.
SafC was incubated in 100 µM MgCl₂, 2 mM DTT, 400 µM SAM, 10 mM HEPES, pH 7.2, 8 fmol (0.5 µCi) [³H]SAM, and different concentrations of either L-dopa or L-dopa-CoA in a total volume of 50 µl at 37°C for 20 min. After incubation, the entire reaction volume was spotted onto a C₈ RP-8 F_254S TLC plate (Merck). The TLC plates were developed in a solvent of n-butanol-water-acetic acid (13:5:2). Following drying, the plate was sprayed with En3Hance (PerkinElmer) and exposed to X-Omat AR film (Kodak) for 17 h. Radiolabeled spots were scraped and quantified using a liquid scintillation counter (Packard).

	RESULTS

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Sequence analysis.
BLASTP searches (http://www.ncbi.nlm.nih.gov) revealed that SafC is closely related to COMTs (58% identity at the amino acid level) and to several methyltransferases from natural product biosynthetic pathways (55% identity at the amino acid level). Using a neighbor-joining tree algorithm available in the ClustalX 1.81 software package (National Center for Biotechnology Information), a phylogenetic tree was generated for SafC and relatives. SafC does not branch with either the COMTs or the natural product genes but instead appears to occupy a distinct branch within this group of proteins.

Related COMTs have been kinetically characterized and shown to specifically 3'-O-methylate caffeoyl-CoA derivatives (35, 36). Hoffmann et al. developed a threading model for the Nicotiana COMT protein, and specific residues were proposed to be responsible for catechol versus CoA binding (17). Those authors then tested their predictions by systematically mutating residues and feeding truncated caffeoyl-CoA analogs to mutant proteins. Importantly, this COMT could not accept unmodified caffeic acid as a substrate. Taken together, the data indicated that specific, predictable residues may be responsible for binding of the CoA motif.

In contrast to the COMTs, much less is known about the related natural product O-methyltransferases found in bacteria. The closest bacterial relatives seem to be involved in the methylation of hydroxymalonyl-acyl carrier protein or hydroxymalonyl-CoA. Indeed, the residues identified by Hoffmann et al. (17) as being important for CoA binding appear to be universal within this group of enzymes, and thus, we predicted that SafC should methylate L-dopa-thioester derivatives specifically. It was predicted that L-dopa-CoA or L-dopa-peptidyl carrier protein (PCP) derivates should be specifically methylated. Since NRPS knockouts in M. xanthus were reported to methylate a substrate (27), the CoA derivative or a PCP thioester from an unclustered part of the pathway seemed likely, since thiolation by SafAB was no longer a possibility.

Synthesis of thioesters.
To mimic the putative CoA- or PCP-bound L-dopa, L-dopa-CoA thioester was synthesized. A variety of methods were attempted to directly thioesterify L-dopa, but they were unsuccessful. The method of Vitali et al. (34) was adapted for synthesis via a thiophenolate intermediate, leading to L-dopa-CoA via a transthiolation reaction. This product could be characterized with proton NMR and mass spectrometry, but its relative instability was problematic for additional characterization and long-term storage. Fresh L-dopa-CoA was therefore prepared immediately prior to assays. The N-acetylcysteamine (SNAC) thioester of L-dopa was prepared in a similar manner (data not shown).

Expression of SafC.
SafC with a C-terminal His₆ tag was readily expressed to high levels in E. coli. The protein was also readily visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after transformation into Streptomyces lividans TK24 by using the pEM4-XH vector. Ni-nitrilotriacetic acid-purified SafC could be stored for several months at –80°C without a noticeable loss in activity. Stored aliquots of SafC were used in a series of experiments to determine substrate selectivity.

Regioselectivity of SafC.
L-Dopa-CoA was used in a large-scale reaction with SafC and SAM, and the reaction was monitored by ¹H NMR. Peak evolution in the aromatic region was observed that corresponded to the production of 3-(3-hydroxy-4-methoxyphenyl)-L-alanine: 6.83 (1H, d, J = 8.4 Hz, C6-H) and 6.64 (1H, s, C2-H) (32). Peaks at 6.63 (1H, d, J = 8.4 Hz, C5-H) overlapped with a decreasing peak from the L-dopa-CoA substrate. Shift assignments were based upon comparison with authentic standards of L-dopa derivatives prepared during previous work by our lab (32), and thus, we could readily distinguish between methylation at oxygen and carbon, as well as regioselectivity, by this method. Further analysis described below revealed that SafC was likely acting directly on the hydrolyzed product, L-dopa, rather than on L-dopa-CoA itself.

HPLC methods were used to assess the products of SafC with either L-dopa or L-dopa-CoA. 4'-O-methylated dihydroxyphenylalanine products were observed from both substrates. No 3'-O-methyl or 5'-methyl products were observed from SafC acting on either substrate. In subsequent kinetics experiments with 5'-methyl-L-dopa, only 4'-O-methyltransferase activity was observed. However, when similar methods were used to test L-tyrosine as a substrate for SafC, no 4'-O-methyltransferase activity was detected.

SafC kinetics and substrate selectivity.
L-Dopa, L-dopa-CoA, catechol, and 5'-methyl-L-dopa were used to assess substrate selectivity (Table 1 and Fig. 2). To our surprise, L-dopa itself was the best substrate, with a k_cat/K_m of 5.5 x 10³ M^–1 s^–1. Catechol incubated with SafC exhibited a k_cat/K_m of 72 M^–1 s^–1.

View larger version (11K): [in this window] [in a new window]   FIG. 2. Substrate selectivity of SafC. (A) L-Dopa is a better substrate than methyldopa, and only catechols are substrates. (B) Catechol, dopamine, and caffeic acid are also substrates, although caffeic acid lacks the strict regioselectivity of the other substrates. The associated plain text numbers indicate kinetic constants (kcat/Km [M–1 s–1]) or relative percent activities in comparison to that of L-dopa (see the text). Me, methyl.

Authentic, enantiopure 5'-methyl-L-dopa was previously synthesized in our lab (32). Although this compound was much more light and base sensitive than L-dopa, it was sufficiently stable for approximate kinetic analysis. The C-methylated dopa derivative was a very poor substrate in comparison to L-dopa, with a k_cat/K_m approximately 2 x 10⁸-fold lower than that for L-dopa. This decrease was due mainly to a greatly lowered k_cat in comparison to that for the native substrate, although K_m was also slightly increased. It is likely that steric interactions with the active site decrease the reaction rate, since the electronics of C-methyl-dopa still greatly favor methylation. The 4'-O-methyl product was the only regioisomer detected in the reaction.

Caffeic acid and dopamine were also substrates for SafC, but full kinetic data were not obtained (Fig. 2). Initial kinetic runs indicated that the reaction of dopamine and caffeic acid with SafC is non-Michaelis-Menten, possibly due to substrate inhibition. However, under optimal conditions, both substrates exhibited approximately 10% of the reaction rate of L-dopa at identical concentrations. For example, at 190 µM, dopamine was converted to 4'-O-methyldopamine at a velocity of 30 nM s^–1 under the same conditions as those used for L-dopa. At 370 µM, caffeic acid was methylated at 40 nM s^–1. For dopamine, only the 4'-O-methylated product was observed, but for caffeic acid, both the 4'- and 3'-O-methyl derivatives were produced at a 3:1 ratio, respectively.

	DISCUSSION

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Although sequence analysis indicates homology between SafC and the group of CoA/pantetheine-dependent methyltransferases, thiolation is not required, and L-dopa itself is the best substrate. The enzyme accepts a variety of catechol substrates, but simple phenol (L-Tyr) is not a substrate. SafC exhibits a regioselectivity different from that of most known catechol methyltransferases, since L-dopa is methylated exclusively at the 4' oxygen. Steric interaction with the catechol moiety appears to be the most important factor for substrate selectivity. The replacement of a single proton with methyl at the 5' carbon of the catechol greatly decreases the turnover number.

Because SafC operates most efficiently on L-dopa, at least in vitro, it is likely that free L-dopa is the natural in vivo substrate of the enzyme. L-Dopa-CoA is probably not a substrate and is at best an extremely poor substrate, and SNAC derivatives were inactive under these conditions. The removal of portions of the alaninyl side chain, as in catechol, caffeic acid, and dopamine, resulted in a loss of 90% of activity, substantiating L-dopa as the enzyme substrate. Further evidence for the intermediacy of L-dopa comes from the knockout experiments of Pospiech et al., who definitively showed that SafC does not act on an intermediate that is covalently bound to the SafAB NRPS (27). Because SafC reacts with L-dopa and not with simple phenols or 5'-methyldopa and because SafC is required for saframycin MX1 production, SafC 4'-O-methylation of L-dopa is likely the first committed step in the biosynthesis of saframycin MX1.

Velasco et al. reported the gene cluster for the production of safracins in Pseudomonas fluorescens (33). Three NRPS genes (sacABC) are homologous in sequence and domain order with safAB. However, there are seven other clustered sequences (sacDEFGHIJ) involved in precursor synthesis and tetrapeptide modification; in contrast, only one modifying gene (safC) is known to be involved in saframycin MX1 production. The two methyltransferases in the sac cluster are not homologous to SafC, and knockout experiments strongly suggest that they do not methylate L-dopa. Instead, they putatively C- and O-methylate free tyrosine, which is later oxidized to the dopa derivative. These differences indicate that the biosyntheses of safracins and saframycins do not follow identical routes. The related metabolite, naphthyridinomycin, contains a similar Tyr-derived unit. Feeding studies demonstrated that either 3'-methyltyrosine or 5'-methyldopa could be incorporated into naphthyridinomycin, indicating that multiple routes to bacterial isoquinoline alkaloids are possible (26).

The ability of SafC to 4'-O-methylate a variety of catechols has potential biotechnological applicability not just in the modification of the saframycin pathway but also in other areas of medicinal and biological research. In particular, dopa and dopamine methylations control the levels of these signaling molecules and are important in neurochemistry (3, 6). The ability of SafC to regioselectively methylate dopamine in a relatively efficient manner indicates that SafC may be a general catalyst of biotechnological importance. 4'-O-methylated dopa and dopamine have been proposed to be important toxins with possible involvement in neurological conditions such as Parkinson's disease (8, 22, 30). The availability of a recombinant, catechol 4'-O-methyltransferase could allow hypotheses about the importance of these molecules to be readily explored.

	ACKNOWLEDGMENTS

 
This work was supported by UU Startup funds to E.W.S. and an NIH Training Grant in Biological Chemistry awarded to J.T.N.

We are grateful to H. Reichenbach for the gift of M. xanthus.

	FOOTNOTES

 
* Corresponding author. Mailing address: Department of Medicinal Chemistry, University of Utah, 30 South 2000 East, Room 201, Salt Lake City, UT 84112. Phone: (801) 585-5234. Fax: (801) 585-9119. E-mail: ews1{at}utah.edu

Published ahead of print on 20 April 2007.

J.T.N. and J.L. contributed equally to this work.

	REFERENCES

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