
DNA Microarray-Based Identification of Serogroups and Virulence Gene Patterns of Escherichia coli Isolates Associated with Porcine Postweaning Diarrhea and Edema Disease
Appl. Environ. Microbiol. Han et al.
73: 4082
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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The O-antigen gene clusters of E. coli O141, O147, O149, O138, O139, O45, O157, and O8 (Fig. S1); agarose gel electrophoresis of multiplex PCR products from O-antigen-specific genes or virulence factor genes (Fig. S2); schematic diagram of the microarray, showing positions of immobilized probes spotted within a single array of the six-array glass slides (Fig. S3); hybridization patterns for representative clinical isolates (Fig. S4)
MS Word document, 4.4MB.
- Supplemental file 2
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Characteristics of the ORFs in the E. coli O141 (Table S1), O147 (Tables S2), and O149 (Table S3) O-antigen gene clusters; all strains used in this study (Table S4); detection of virulence genes by conventional PCR (Table S5); detection results for 17 porcine fecal samples (Table S6)
MS Word document, 462K.