Files in this Data Supplement:

• Supplemental file 1 - Activity and dehydration levels of nisin ring A (Table S1) and ring B (Table S2) mutants; monoisotopic masses of the truncated nisin ring B PH mutant, which lacks two thioether rings and contains a single disulfide bridge (Table S3); characterization of truncated nisin ring A and B mutants by mass spectrometry (Table S4); characterization of truncated nisin mutants by postsource decay (Table S5).
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