Files in this Data Supplement:

• Supplemental file 1 - Additional materials and methods: screening for dinoflagellate actin(s) by RT-PCR, DNA cloning and sequencing, detection of actin and tubulin expression in P. piscicida dinospores and cysts by Western blotting, and visualization of actin and tubulin in P. piscicida intact dinospores; results for partial actin sequencing of P. piscicida, Rhodomonas sp., and other dinoflagellate cDNAs; alignment of Alveolata actin genes for designing degenerate primers (Fig. S1); design of specific PCR primers for P. piscicida and Rhodomonas sp. (CCMP768) actins (Fig. S2); demonstration of specific amplification of P. piscicida (left) and Rhodomonas (right) actins and chloroplastic GAPDHs (Fig. S3).
  Zipped PDF document, 659K.
• Supplemental file 2 - Detection of actin and tubulin of P. piscicida (Fig. S4).
  Zipped PDF document, 266K.
• Supplemental file 3 - Differentiation of Pfiesteria dinospores by lectin staining (Fig. S5).
  Zipped PDF document, 5.1MB.