Design and Application of Highly Responsive Fluorescence Resonance Energy Transfer Biosensors for Detection of Sugar in Living Saccharomyces cerevisiae Cells
Appl. Environ. Microbiol. Ha et al.
73: 7408
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Additional methods: construction of FRET-based maltose and sucrose sensors, saturation mutagenesis of L1-linker moiety and binding site, expression and purification of sensor proteins, and molecular simulation of the composite linker moiety.
Zipped PDF document, 156K.
- Supplemental file 2
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Oligonucleotide sequences used for cloning and mutagenesis (Table S1); schematic drawing of maltose sensors and reassembly for novel specificity (Fig. S1); comparison of substrate specificities (Fig. S2); gene construction strategies (Fig. S3).
Zipped PDF document, 163K.
