Supplemental material

Files in this Data Supplement:

• Supplemental file 1 - Additional methods: construction of FRET-based maltose and sucrose sensors, saturation mutagenesis of L₁-linker moiety and binding site, expression and purification of sensor proteins, and molecular simulation of the composite linker moiety.
  Zipped PDF document, 156K.
• Supplemental file 2 - Oligonucleotide sequences used for cloning and mutagenesis (Table S1); schematic drawing of maltose sensors and reassembly for novel specificity (Fig. S1); comparison of substrate specificities (Fig. S2); gene construction strategies (Fig. S3).
  Zipped PDF document, 163K.