Files in this Data Supplement:

• Supplemental file 1 - Plasmids used this study (Table S1); positions within G2-EPSPS that tolerate five amino acid insertions and secondary structure prediction results (Table S2a); sites within G2-EPSPS that do not tolerate five amino acid insertions and secondary structure prediction results (Table S2b); intein-mediated protein complementary of G2-EPSPS protein in E. coli ER2799 (Fig. S1); construction of plasmid pMEPSc296Ic (Fig. S2a); construction of plasmid pMEPSN295IN (Fig. S2b); growth of E. coli ER2799 and its transformants containing different plasmids in M9 solid minimal media supplemented with 50 mM glyphosate and 0.3 mM IPTG (Fig. S3).
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