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Use of Transposon Promoter-Probe Vectors in the Metabolic Engineering of the Obligate Methanotroph Methylomonas sp. Strain 16a for Enhanced C40 Carotenoid Synthesis
Appl. Environ. Microbiol. Sharpe et al. 73: 1721

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Supplemental material (PCR primers used to determine transposon insertion sites, recipe for Methylomonas growth medium, construction of promoterless carotenoid transposon delivery vectors, examples of carotenoid gene expression in Methylomonas, methods for construction of carotenoid expression plasmids, and methods for construction of integration vector used in metabolic engineering of Methylomonas sp.)
    Word document, 1.7MB.



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