Files in this Data Supplement:

• Supplemental file 1 - Fig. S1 (Impact of library amplification on relative population within that library), S2 (Impact of increasing amounts of butanol on growth and metabolite accumulation), S3 (Progression of enrichment for genes most enriched as of the final transfer), and S4 (Comparison of batch growth characteristics of C. acetobutylicum pCAC1869 and C. acetobutylicum pIMP1) and Tables S1 (Identity, length, and chromosomal location of sequenced inserts enriched from a single batch challenge), S2 (Key metabolite measurements of serial, stationary-phase transfers of challenge cultures into media containing increasing butanol levels), and S3 (Relative ranks and absolute intensities of the top 70 genes as of the final transfer)
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