Evaluation of PCR Primer Selectivity and Phylogenetic Specificity by Using Amplification of 16S rRNA Genes from Betaproteobacterial Ammonia-Oxidizing Bacteria in Environmental Samples
Appl. Environ. Microbiol. Junier et al.
74: 5231
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
BLAST results and group designations of unique sequences in clone libraries from the metalimnetic layer of Lake Kinneret (Table S1), from the sediment-water interface of Lake Plußsee (Table S2), from the Baltic Sea at 20 m (Table S3), and from the Baltic Sea sediment-water interface (Table S4); AOB in published clone libraries of PCR products prepared with different primer combinations (Table S5); theoretical comparison of primer sequences with 16S rRNA gene sequences from cultured AOB (Table S6).
Zipped MS Word document, 30K.
