Files in this Data Supplement:

• Supplemental file 1 - Primers used in this study (Table S1); plasmids used in this study (Fig. S1); a Hill function accurately fits relationship between corrected absorbance and CFU/ml in LBM medium (Fig. S2); periodic dilution results for network architecture c, pluxRp-101 and pPSSUB-102 (Fig. S3); periodic dilution results for network architecture b, pluxG-102C and pPSSUB-102 (Fig. S4); individual periodic dilution experiments for testing of architecture b (pluxG-102C and pPSSUB-102) for bistability (Fig. S5); cell viability for periodic dilution experiments as determined by serial dilution and plating (Fig. S6); derivation of the quorum-sensing mathematical model (section S1); kinetic mechanism used to model behaviors of network architecture b (Table S2); parameters used for simulation of quorum-sensing models (Table S3); effect of positive feedback on the p(luxR) promoter (section S2); kinetic mechanisms used to model behaviors of the three network architectures (Table S4); effect of increasing the amplication factor ;alpha_{p(luxR),/sub>} for the p(luxR) promoter for network architectures a and b (Fig. S7); effect of prolonged quorum-sensing induction on architecture c (section S3); steady states of pluxRp-103E and pPSSUB-102 as a function of 3OC6HSL induction (Fig. S8); periodic dilution results for pluxRp-103E and pPSSUB-102 as a function of 3OC6HSL induction (Fig. S9).
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