Files in this Data Supplement:

• Supplemental file 1 - Description of computational analysis of the ECOM3 phenotypic behavior conducted using an iAF1260 genome-scale metabolic model; sequences of primers used for gene knockout generation and conformation; computationally predicted fluxes for lactate production and growth rate of ECOM3 phenotype as a result of the perturbation screen; growth rate response to the amount of supplementation during the adaptive evolution experiment; heterogeneity of the three evolved populations based on the final lactic acid concentration measured after 8 h of high-throughput fermentation.
  PDF file, 142K.