Files in this Data Supplement:

• Supplemental file 1 - ¹H and ¹³C NMR data for asperthecin (Table S1); list of primers used in this study (Table S2); list of genes near the PKS of the asperthecin synthase gene cluster (Table S3); UV-visible absorbance, mass spectrometry positive mode, and mass spectrometry negative mode characterization for austinol, dehydroaustinol, sterigmatocystin, and asperthecin (Fig. S1); HMBC correlations of asperthecin (Fig. S2); HPLC analysis of deletions of genes surrounding the asperthecin PKS (Fig. S3); ¹H NMR spectrum (Fig. S4) and ¹³C NMR spectrum (Fig. S5) of asperthecin; comparison of secondary metabolite profiles of TN02A7, the nkuAΔ strain used as a parental strain for these studies, and R153, an nkuA⁺ control strain (Fig. S6).
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