Use of a Recombinant Fluorescent Substrate with Cleavage Sites for All Botulinum Neurotoxins in High-Throughput Screening of Natural Product Extracts for Inhibitors of Serotypes A, B, and E
Appl. Environ. Microbiol. Hines et al.
74: 653
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Description of the cloning, expression, and purification of the recombinant fluorescent substrate for the protease activities of all seven botulinum neurotoxin serotypes, with sources of plasmids, bacterial strains, and purification media noted, and description of the method employed to purify the expressed protein product; sequences of primers (Table S1); SDS-PAGE of crude extract, a fraction from an intermediate purification step, and the final purified product (Fig. S1).
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