Files in this Data Supplement:

• Supplemental file 1 - Detailed construction of the A. baylyi BD413-based marker-reporter rescue system; marker rescue transformation frequencies of A. baylyi BD413(rbcL-ΔPaadA::gfp) in vitro; transformation frequencies with bacterial DNA or cell lysates (Table S1); transformation frequencies with plasmid pCLT and different sources of transplastomic and wild-type donor DNA (Table S2); epifluorescence and confocal laser scanning microscopy methodologies for visualization and quantification of in vitro transformation of A. baylyi BD413(rbcL-ΔPaadA::gfp) with cell lysates of E. coli (pCLT); preparation of an artificial system simulating natural leaf decaying; transformation frequencies on simulated tobacco-decaying tissues (Table S3).
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