
A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16
Appl. Environ. Microbiol. Tang et al.
74: 1567
Supplemental material
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Determination of degradation of HSP by resting cells of transformant GTPF at 37°C in aqueous phase by HPLC analysis (Fig. S1A) and HPLC spectrum of HSP and DHP during the degradation reaction by transformant GTPF (Fig. S1B); determination of degradation of HSP by crude cell extracts of transformant pET27b-hsp at 37°C in aqueous phase by HPLC analysis (Fig. S2); HPLC spectrum of degradation of HSP by crude cell extracts of transformant pET-27b(+) (A) and transformant pET27b-hsp (B) at 37°C in aqueous phase (Fig. S3).
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