Files in this Data Supplement:

• Supplemental file 1 - Amino acid sequence comparison of PlyB and PlyBeta (Fig. S1); Coomassie-stained SDS-PAGE gel of metagenomic aerolysin, secreted by the host E. coli and purified by ammonium sulfate precipitation and anion-exchange chromatography (Fig. S2); sequence-based analysis of metagenomic DNA extracted from the gut of a single Eisenia hortensis worm, indicating a predominantly bacterial origin (Table S1).
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