Files in this Data Supplement:

• Supplemental file 1 - Comparison of all JCVI CDSs with 641 CDSs for hypothetical proteins occurring in D. vulgaris only (Fig. S1).
  Zipped TIF file, 97K.
• Supplemental file 2 - Hybridization of D. vulgaris Hildenborough microarray slide with labeled genomic DNA (Fig. S2).
  Zipped TIF file, 4.3MB.
• Supplemental file 9 - Survey of highly expressed genes of D. vulgaris as well as the consensus codon usage table derived from these genes (Table S1).
  Zipped MS Word document, 11K.
• Supplemental file 3 - Sequences of 70-mer probes designed for 2,824 CDSs of D. vulgaris Hildenborough (Table S2).
  Zipped MS Excel file, 180K.
• Supplemental file 4 - D. vulgaris Hildenborough ORFs without strong homologies to known genes (Table S3).
  Zipped MS Excel file, 46K.
• Supplemental file 5 - Complete microarray data set showing log2 of expression ratios for CP vs 50% hydrogen, CP vs 5% hydrogen, and 50% hydrogen vs lactate (Table S4).
  Zipped MS Excel file, 242K.
• Supplemental file 6 - Fraction of biofilm RNA vs total RNA extracted from bioreactors (Table S5).
  Zipped RTF file, 9K.
• Supplemental file 7 - Overlap and relative positions of SAMF vs VIMSS probes (Table S6).
  Zipped MS Excel file, 59K.
• Supplemental file 8 - Comparison of gene expression changes in D. vulgaris biofilms generated by Zhang et al. (41) and in the current study.
  Zipped MS Excel file, 11K.
• Supplemental file 10 - Legends to Fig. S1 and S2 and Tables S1 to S7.
  Zipped MS Word document, 4K.