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Applied and Environmental Microbiology, April 2008, p. 2454-2460, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.01573-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Responses of Bacillus subtilis to Hypotonic Challenges: Physiological Contributions of Mechanosensitive Channels to Cellular Survival{triangledown}

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, and Erhard Bremer*

Department of Biology, Philipps University Marburg, 35032 Marburg, Federal Republic of Germany

Received 11 July 2007/ Accepted 20 February 2008


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ABSTRACT
 
Mechanosensitive channels are thought to function as safety valves for the release of cytoplasmic solutes from cells that have to manage a rapid transition from high- to low-osmolarity environments. Subsequent to an osmotic down-shock of cells grown at high osmolarity, Bacillus subtilis rapidly releases the previously accumulated compatible solute glycine betaine in accordance with the degree of the osmotic downshift. Database searches suggest that B. subtilis possesses one copy of a gene for a mechanosensitive channel of large conductance (mscL) and three copies of genes encoding proteins that putatively form mechanosensitive channels of small conductance (yhdY, yfkC, and ykuT). Detailed mutational analysis of all potential channel-forming genes revealed that a quadruple mutant (mscL yhdY yfkC ykuT) has no growth disadvantage in high-osmolarity media in comparison to the wild type. Osmotic down-shock experiments demonstrated that the MscL channel is the principal solute release system of B. subtilis, and strains with a gene disruption in mscL exhibited a severe survival defect upon an osmotic down-shock. We also detected a minor contribution of the SigB-controlled putative MscS-type channel-forming protein YkuT to cellular survival in an mscL mutant. Taken together, our data revealed that mechanosensitive channels of both the MscL and MscS types play pivotal roles in managing the transition of B. subtilis from hyper- to hypo-osmotic environments.


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INTRODUCTION
 
The gram-positive soil bacterium Bacillus subtilis has to frequently manage the transition from high- to low-osmolarity surroundings due to drying and flooding of the upper layers of the soil (7, 35). To fend off the negative effects of high osmolarity on cellular water content and cell physiology, B. subtilis accumulates compatible solutes through either synthesis or uptake from the environment (7, 20). Compatible solutes are highly soluble organic osmolytes (e.g., proline and glycine betaine) that can be amassed by the cell to exceedingly high levels without interfering with cell physiology (12). In this way, the water content of the cell is balanced with the prevalent osmolarity of the environment and cell turgor is stabilized. Consequently, cell growth can occur under osmotically unfavorable conditions.

The massive accumulation of compatible solutes permits cell survival under hypertonic circumstances. However, the very same compounds become a threat to the integrity of the cell when B. subtilis is suddenly exposed to low-osmolarity surroundings via rainfall or washout into fresh water sources (8, 35). The compatible solutes accumulated by the cell increase the osmotic potential of the cytoplasm and thereby instigate an osmotically controlled water influx (36). This water influx drives up turgor to nonphysiological high values and in extreme cases can lead to cell lysis (4, 6). Various researchers have observed that a number of cytoplasmic solutes, including proline, glycine betaine, potassium, glutamate, trehalose, and ATP, are rapidly released from osmotically downshifted cells (3, 9, 29, 30). It is generally thought that mechanosensitive channels located in the cytoplasmic membrane are responsible for the rapid release of the various compounds. Mechanosensitive channels thus appear to act as safety valves against a strong buildup of turgor, and their gating activities prevent cell lysis upon a severe osmotic down-shock (4, 5, 22, 25, 27).

The best-characterized bacterial mechanosensitive channels are the channels of large conductance (MscL) and small conductance (MscS): for both of these, crystal structures have been obtained (2, 10). In response to changes in the lipid bilayer tension, the MscL and MscS channel proteins gate and they open transiently to form large aqueous pores, through which both solutes and solvents can pass rapidly. Consequently, the concentration of water-attracting solutes inside the cells is lowered, turgor is reduced, and cell lysis is prevented (5, 22, 25). The structural genes for the MscL and MscS channel-forming proteins have been initially identified in Escherichia coli (21, 31), and homologues of these genes have been found in a variety of microbial species (27). Disruption of either the E. coli mscL or the mscS gene displays no obvious phenotype, but in an elegant study, Levina et al. (21) demonstrated that an mscL mscS double mutant cannot effectively manage the transition from hyperosmotic to hypo-osmotic conditions and most of the cells lyse.

Mechanosensitive channel activity in B. subtilis was originally discovered by Zoratti et al. (37) and Szabo et al. (32), using patch clamp experiments with giant protoplasts. Evidence for the presence of ion channels in the B. subtilis plasma membrane was also obtained by Alcayaga et al. (1) in electrophysiological studies using fusions of plasma membrane vesicles with planar lipid bilayers. The channels detected in these studies showed a wide range of conductances and kinetic behaviors, suggesting that more than one channel-forming protein operates in B. subtilis. The activity of the stretch-activated channels of B. subtilis could be inhibited by gadolinium ions (3), an effective blocker of stretch-activated channels in eucaryotes. The B. subtilis genome contains one copy of the mscL gene. Heterologous expression of mscL in E. coli and analysis of the recombinant strain by patch clamp studies revealed that the B. subtilis mscL gene encodes a functional mechanosensitive channel (24). Recent data by Wahome and Setlow (34) strongly suggest an important role for the MscL channel in the management of the transitions of log-phase cells of B. subtilis from high- to low-osmolarity surroundings. Database searches conducted by Pivetti et al. (27) revealed the existence of three genes (yfkC, ydhY, and ykuT) in the B. subtilis genome whose deduced protein products display homology to the E. coli MscS protein (21). No functional analysis of the yfkC, ydhY, and ykuT genes in the B. subtilis osmoadaptation response has been reported.

To study the adaptation reaction of B. subtilis in response to a rapid transition from hyperosmotic to hypo-osmotic circumstances, we constructed a comprehensive set of single and multiple gene disruption mutations in the mscL gene and the three genes (yfkC, ydhY, and ykuT) encoding the putative mechanosensitive channels of small conductance (MscS type) and analyzed their properties.


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MATERIALS AND METHODS
 
Media, growth conditions, and chemicals.
The various B. subtilis strains were grown in Spizizen's minimal medium (SMM), with 0.5% (wt/vol) glucose as the carbon source. The medium was supplemented with L-tryptophan (20 mg liter–1), L-phenylalanine (18 mg liter–1), and a solution of trace elements (16). Cells were routinely grown aerobically at 37°C in 100-ml Erlenmeyer flasks with a culture volume of 20 ml in a shaking water bath set at 230 rpm. The growth of the bacterial cultures was monitored spectrophotometrically at a wavelength of 578 nm (OD578). The salinity of bacterial cultures was raised by adding appropriate volumes of NaCl from a 5 M stock solution. The osmolarities of growth media were determined with a vapor pressure osmometer (model 5500; Wescor). Antibiotics for B. subtilis strains carrying antibiotic resistance cassettes integrated into the genome were used at the following concentrations: chloramphenicol, 5 µg ml–1; spectinomycin, 100 µg ml–1; tetracycline, 15 µg ml–1; and erythromycin, 0.4 µg ml–1.

[1-14C]glycine betaine (2.035 GBq mmol–1; 3.7 MBq ml–1) was purchased from American Radiolabeled Chemicals Inc. (St. Louis, MO). Glycine betaine was obtained from Sigma (Deisenhofen, Germany).

Survival assays of B. subtilis mutant strains exposed to osmotic downshifts.
The cells were cultured in SMM with 0.8 M NaCl until they reached mid-exponential growth phase (OD578 = 0.6 to 0.8). To determine the number of viable cells, samples were withdrawn from the cultures and appropriate dilutions were plated onto SMM plates containing 0.8 M NaCl to avoid an osmotic down-shock of the cells. After incubation for 48 h at 37°C, colonies were counted and this total cell number was set at 100% of preshocked cells for each individual experiment. To perform a hypo-osmotic shock experiment, 1 ml of the cell culture (grown in SMM with 0.8 M NaCl) was withdrawn and the cells were subjected to an osmotic down-shock by diluting the culture into 19 ml of prewarmed (37°C) SMM containing various concentrations of NaCl. These cells were then incubated with shaking at 37°C for 30 min to allow adjustment to the new osmotic conditions. Subsequently, 100-µl aliquots were withdrawn from the cultures, serial dilutions were prepared in isotonic growth medium, and the cells were plated onto SMM plates containing appropriate amounts of NaCl to avoid a further osmotic downshift. The plates were incubated for 2 days at 37°C before the colonies were counted.

Bacterial strains, plasmids, and construction of B. subtilis mutants.
The B. subtilis strains used in this study are all derivatives of the wild-type strain JH642 (trpC2 pheA1) (BGSC 1A96), a kind gift from J. Hoch (La Jolla, CA). Chromosomal mutations in the mscL (ywpC), ydhY, yfkC, and ykuT genes were constructed by marker replacement via homologous recombination with plasmid-encoded gene disruption mutations carrying antibiotic resistance markers (11). For the construction of these mutants, plasmids pSM33 (mscL::spc), pSM41 [{Delta}(yfkC::tet)], pSM44 [{Delta}(ydhY::ery)], and pSM47 [{Delta}(ykuT::cat)] (see Fig. 2) were linearized by cutting with a restriction enzyme, the B. subtilis wild-type strain JH642 was then transformed with linear plasmid DNA, and the desired B. subtilis mutants were selected by plating the transformation mixture onto LB plates containing the appropriate antibiotic. This yielded the following mutant strains: SMB53 (mscL::spc), SMB58 [{Delta}(ydhY::ery)], SMB62 [{Delta}(yfkC::tet)], and SMB63 [{Delta}(ykuT::cat)]. The correct physical structures of the chromosomal gene disruptions in these strains were verified by Southern hybridization using probes derived from plasmids pSM31 (mscL+), pSM38 (yfkC+), pSM39 (yhdY+), and pSM40 (ykuT+). These plasmids were constructed as follows. In each case, the target gene or gene fragments were amplified by PCR using appropriately chosen primers (data not shown). Plasmid pSM31 is a derivative from pBSK– (Stratagene, Heidelberg, Germany) carrying the 'ywpB-mscL-'ywpD region; plasmid pSM38 is a derivative of pBSK– carrying the 'yfkB-yfkC-'yfkD region; plasmid pSM39 is a derivative of pPD100 (13, 15) carrying the 'yhdX-yhdY-yhdZ-'yheN region, and plasmid pSM40 is a derivative of pBSK– carrying the ykuU'-ykuT-'ykuS region. Into these plasmids, antibiotic resistance cassettes were inserted as summarized in Fig. 2. Cassettes conferring resistance to spectinomycin (spc), tetracycline (tet), erythromycin (ery), and chloramphenicol (cat) were cut by restriction digests out of plasmids pDG1726 (15), pDG1515 (15), pBKB80 (B. Kempf and E. Bremer, unpublished data), and pJMB1 (M. Jebbar and E. Bremer, unpublished data), respectively.


Figure 2
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  		FIG. 2. Genetic organization of the B. subtilis mscL, ykuT, yhdY, and yfkC genes. The genetic organization of the mscL, ykuT, yhdY, and yfkC loci on the B. subtilis chromosome are shown. The names of the plasmids used for chromosomal marker replacement are given. The positions of the restriction sites relevant for the construction of gene disruptions are indicated. The antibiotic resistance cassettes used to disrupt the mscL, ykuT, yhdY, and yfkC genes are given; the sizes of the antibiotic resistant cassettes are not drawn to scale.

For Southern hybridization, a nonradioactive digoxigenin DNA labeling and detection kit (Roche, Mannheim, Germany) was used for the labeling of restriction fragments with digoxigenin-dUTP. Chromosomal DNA was isolated from the various B. subtilis mutant strains and transformed into appropriate chosen recipient B. subtilis strains to construct combinations of gene disruption mutations. The collection of strains constructed in this way is listed in Table 1. To construct a mutant that was simultaneously defective in mscL and sigB, we isolated chromosomal DNA from the B. subtilis mutant BLOB22 (sigB{Delta}2::cat) (33) and transformed strain SMB53 (mscL::spc). Chloramphenicol-resistant colonies were selected, and one of these colonies was designated strain THB7 (mscL::spc sigB{Delta}2::cat).


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  		TABLE 1. Strains with mutations in putative channel-forming genes

Release of radiolabeled glycine betaine from osmotically down-shocked cells.
The various strains were cultured at 37°C in a shaking water bath in 100-ml flasks containing 40 ml SMM supplemented with 0.8 M NaCl to raise the osmolarity until the cells reached mid-exponential growth phase (OD578 = 0.6 to 0.8). Glycine betaine (1 mM) and 1 µM [14C]glycine betaine were then added to the culture (corresponding to a specific activity of 40.7 kBq per culture), and the cells were further grown for 120 min to allow glycine betaine uptake. During this period, glycine betaine uptake activity reached saturation (Fig. 1A). Subsequently, 4-ml aliquots of the cells were distributed to several Falcon tubes, the OD578 was determined, and the cells were then exposed to osmotic downshifts of various degrees by diluting the culture with SMM containing various amounts of NaCl. To monitor uptake and expulsion of [14C]glycine betaine by the B. subtilis cells, 300-µl aliquots were withdrawn from the cultures at time intervals and filtered through Millipore membranes (0.45 µm). The cells retained by the filters were washed twice with 3 ml of an isotonic SMM medium, and the radioactivity remaining in the cells was determined by scintillation counting.


Figure 1
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  		FIG. 1. Release of glycine betaine from osmotically down-shocked cells. The B. subtilis wild-type strain JH642 was grown in SMM with 0.8 M NaCl in the presence of 1 mM glycine betaine and 1 µM [14C]glycine betaine until the uptake of glycine betaine reached a steady state. Cells were then subjected to osmotic downshifts of various degrees by dilution into SMM containing the appropriate amounts of NaCl. (A) Representative time course of the release and reaccumulation of glycine betaine in cells subjected to an osmotic down-shock equivalent to the withdrawal of 0.38 M NaCl. The arrow marks the time point of the down-shock. (B) To visualize the time course of the release of glycine betaine from the cells immediately after the osmotic downshift, the measured data points shown in panel A were replotted on a larger time window. (C) The release of glycine betaine is dependent on the degree of the down-shock. The amount of glycine betaine released by the cells immediately after the osmotic downshift was calculated in relation to the maximal glycine betaine concentration accumulated before the osmotic down-shock that was set as 100%. The data given in panel C are the means of three independent experiments.


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RESULTS AND DISCUSSION
 
Release of glycine betaine upon a hypo-osmotic shock.
B. subtilis cells growing under hyperosmotic conditions can accumulate large amounts of the compatible solute glycine betaine from environmental sources via the osmoregulated OpuA, OpuC, and OpuD transport systems (17-19). This leads to intracellular glycine betaine pools that are linearly correlated with the external osmolarity and reach values of 1.2 M to 1.3 M in cells cultivated in SMM with 1 M NaCl (S. Moses, E. P. Bakker, and E. Bremer, unpublished results).

We were interested in the release of glycine betaine from cells grown at high osmolarity that subsequently experienced an osmotic down-shock. To monitor expulsion of glycine betaine, we grew the B. subtilis wild-type strain JH642 to mid-exponential growth phase (OD578 = 0.6 to 0.8) in a minimal medium (SMM) whose osmolarity had been increased with 0.8 M NaCl (final osmolarity, 1,900 mosmol/kg of water) to trigger uptake of glycine betaine from the medium. A mixture of radiolabeled glycine betaine (1 µM) and nonlabeled glycine betaine (1 mM) was added to the cells, and the culture was further grown for 120 min until glycine betaine uptake activity reached saturation (Fig. 1A). We then subjected these actively growing cells to an osmotic down-shock equivalent to the withdrawal of 0.38 M NaCl from the culture. The amount of radiolabeled glycine betaine remaining in the cells was measured after the osmotic down-shock over a 20-min time period by scintillation counting. Immediately after the osmotic down-shock, the cells ejected about 70% of the initially accumulated radiolabeled glycine betaine (Fig. 1A and B). However, they quickly reaccumulated part of the released glycine betaine until a new steady-state level was reached (Fig. 1A and B).

We then performed a series of experiments of this type where the culture was suddenly diluted into SMM supplemented with different concentrations of NaCl (0 to 0.8 M NaCl), thereby generating osmotic down-shocks of different degrees. The release of radiolabeled glycine betaine from the cell immediately after the osmotic down-shock was plotted versus the degree of the osmotic downshift (Fig. 1C). With osmotic decreases equivalent to the withdrawal of 0.285 M NaCl, there was only a minor overall release of glycine betaine from the cells, indicating that B. subtilis cells can withstand moderate osmotic downshifts without losing much of the previously accumulated compatible solutes. A further increase in the strength of the osmotic down-shock triggered a substantial efflux of glycine betaine. The amount of glycine betaine released from the cells was correlated with the severity of the osmotic down-shock (Fig. 1C), thereby allowing the cell a graded response to osmotic changes in its environment. Almost the entire amount of preaccumulated glycine betaine was released from the cells when the osmotic downshift was equivalent to the withdrawal of at least 0.57 M NaCl (Fig. 1C). Release of glycine betaine from the osmotically downshifted B. subtilis cells was not caused by cell lysis, since the wild-type strain JH642 can sustain an osmotic down-shock equivalent to the withdrawal of 0.76 M NaCl with only marginal loss of cell viability (see below and Fig. 3). It is thus apparent from the experiments whose results are documented in Fig. 1 that osmoregulating B. subtilis cells sensitively readjust their intracellular compatible solute pool in tune with decreases in medium osmolarity.


Figure 3
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  		FIG. 3. Survival of mscL, yfkC, yhdY, and ykuT B. subtilis mutant strains following an osmotic downshift. The cells were pregrown in SMM with 0.8 M NaCl until they reached an OD578 of 0.6 to 0.8. The cultures were then subjected to an osmotic down-shock by a 20-fold dilution in SMM without salt (resulting in an osmotic down-shock equivalent to the withdrawal of 0.76 M NaCl). Cellular survival of the osmotic down-shock is expressed in percentages relative to the viable cell number before the osmotic down-shock. The data given are the means of three independent experiments.

Construction of mutations in genes for putative mechanosensitive channel-forming proteins.
B. subtilis contains one MscL-type mechanosensitive channel (24), which is encoded by the mscL (ywpC) gene located at position 319.7° on the genetic map of B. subtilis. Database searches and phylogenetic analysis performed by Pivetti et al. (27) suggest the existence of three homologues of the E. coli MscS (YggB) channel protein in B. subtilis. These are the yhdY gene at position 88.7°, the yfkC gene at position 74.1°, and the ykuT gene at position 127.4° on the B. subtilis chromosome. The B. subtilis YkuT (267 amino acids) and YfkC (280 amino acids) proteins are of sizes similar to that of the E. coli MscS (YggB) protein (286 amino acids) and exhibit moderate protein sequence identities of 22% and 14%, respectively, to MscS. The YkuT and YfkC proteins have predicted topologies similar to that known for the MscS protein (2, 23), with three membrane-embedded protein segments. The YkuT and YfkC proteins exhibit 28% protein sequence identity to each other, and major regions of sequence identity occur in the predicted membrane-spanning segments. A third MscS-related protein, YhdY, has been noted in B. subtilis by Pivetti et al. (27). However, YhdY (370 amino acids) is clearly different from YkuT and YfkC since YhdY contains five predicted membrane-spanning sequences and a very long carboxy-terminal tail (191 amino acids), which is predicted to face the outside of the cytoplasmic membrane. Its overall sequence identities to YkuT (13%) and YfkC (14%) from B. subtilis and MscS (6%) from E. coli are modest.

To study the physiological functions of the of B. subtilis mscL (ywpC), ykuT, yfkC, and yhdY genes, we constructed an isogenic set of mutants derived from the wild-type strain JH642 that carried different antibiotic resistance markers in each of these genes (Fig. 2). The antibiotic resistance markers used for these strain constructions were chosen in such a way that the various mutations in the putative channel-forming genes could be readily combined by genetic transformation of the wild-type B. subtilis strain with chromosomal DNA to give appropriate double-, triple-, and quadruple-mutant strains (Table 1).

To test whether mutations in the putative channel-forming genes mscL, ykuT, yfkC, and yhdY influence cell growth at high osmolarity, we used the quadruple-mutant strain SMB80 (Table 1) and compared its growth properties to those of the wild-type strain JH642 in a minimal medium with 1.2 M NaCl. The mutant strain SMB80 exhibited the same growth characteristics as the parent strain JH642 (data not shown), demonstrating that the channel protein MscL and the putative MscS-type channel proteins YkuT, YfkC, and YhdY are dispensable for growth of B. subtilis in high-osmolarity environments. This quadruple-mutant strain (SMB80) and the single-mutant strains also did not exhibit any growth phenotype when they were cultured in SMM minimal medium without added salt (data not shown).

Physiological responses of mechanosensitive channel mutant strains to severe osmotic down-shocks.
We first tested the survival of the B. subtilis mscL (ywpC), yhdY, yfkC, and ykuT single-mutant strains upon a severe osmotic downshift. For these experiments, the cells were grown to mid-exponential phase (OD578 = 0.6 to 0.8) in SMM with 0.8 M NaCl and were suddenly diluted to low-osmolarity conditions (SMM with 0.04 M NaCl). This drastic drop in osmolarity did not impair the colony-forming ability of strain JH642 (Fig. 3), indicating that a B. subtilis wild-type strain can sustain severe osmotic downshifts with only a marginal effect on cell viability. This also has been independently observed by Wahome and Setlow (34). The single-mutant strains SMB58 (yhdY), SMB62 (yfkC), and SMB63 (ykuT) exhibited the same survival capacity as the wild-type strain JH642 upon a drastic osmotic downshift. In contrast, approximately 70% of the cells of the mscL (ywpC) mutant SMB53 were killed by such a down-shock (Fig. 3), demonstrating that the integrity of the MscL channel-forming protein is critical for the effective management of a hypo-osmotic shock by the B. subtilis cells. This situation is similar to that found by Folgering et al. (14) in Lactococcus lactis, where also an MscL-type channel functions as the main solute release system upon an osmotic downshift. This differs from the situation observed in E. coli where an mscL mutant by itself has no obvious phenotype (21).

We also constructed an yhdY yfkC double mutant (strain SMB67) and an yhdY yfkC ykuT triple mutant (SMB76) and assessed their survival upon a severe osmotic downshift. Neither the double mutant nor the triple mutant was affected by the osmotic down-shock (Fig. 3). Hence, B. subtilis can effectively manage a rapid transition from high to low osmolarity as long as the MscL channel is intact.

We then individually combined the mscL (ywpC) mutation with single mutations in ydhY, yfkC, and ykuT and tested the sensitivity of these double-mutant strains to an osmotic downshift. The double-mutant strains SMB64 (yhdY mscL) and SMB65 (yfkC mscL) exhibited the same phenotype as the mscL (ywpC) single-mutant strain SMB53 (Fig. 3). However, when the ykuT mutation was combined with the mscL mutation (strain SMB66), there were essentially no surviving cells subsequent to an osmotic down-shock. Hence, while the ykuT mutation by itself (strain SMB63) has no significant negative effect on cell survival (Fig. 3), its combination with an mscL mutation enhanced the osmosensitivity of mscL mutants subsequent to a hypo-osmotic shock.

mscL mutants are sensitive to osmotic stress rather than salt stress.
To test if the killing of strains carrying mscL mutations by osmotic downshifts was specific to cultures grown in the presence of the ionic osmolyte NaCl or represented a true osmotic effect, we grew the various mutant strains in SMM in the presence of 0.9 M sucrose and then subjected these cultures to osmotic downshifts. The same phenotype as that in the above-reported experiments with NaCl-grown cultures was observed, indicating that the sensitivity of mscL mutants to an osmotic down-shock is related to osmotic and not ionic strength.

Killing of the mscL ykuT double mutant SMB66 in response to graded hypo-osmotic challenges.
The above-presented data on the release of glycine betaine from osmotically down-shocked cells suggest that the mechanosensitive channels from B. subtilis open when the cultures are subjected to an osmotic down-shock equivalent to the withdrawal of >0.285 M NaCl (Fig. 1C). To further corroborate this finding, we carried out a series of osmotic downshift experiments where we graded the degree of osmotic down-shocks in small increments. For these experiments, we focused on the mscL ykuT double-mutant strain SMB66 since it exhibited the strongest phenotype of all strains that we tested in response to osmotic downshifts (Fig. 4). SMB66 tolerated osmotic downshifts equivalent to the withdrawal of 0.333 M NaCl without significant loss of viability. However, when the osmotic down-shock exceeded this value, the survival of SMB66 decreased in parallel with increases in the magnitude of the osmotic downshift (Fig. 4). We therefore conclude from these data that B. subtilis can readily withstand an osmotic down-shock equivalent to the withdrawal of 0.333 M NaCl in the absence of the MscL and YkuT proteins, but larger drops in the osmolarity are detrimental to cell viability.


Figure 4
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  		FIG. 4. Survival of the mscL ykuT double-mutant strain SMB66 in response to hypo-osmotic down-shocks of different degrees. A culture of strain SMB66 (mscL ykuT) was pregrown in SMM with 0.8 M NaCl until the culture had reached an OD578 of 0.6 to 0.8. Osmotic down-shocks of various degrees were then performed by diluting 1 ml of culture in 19-ml volumes of SMM media containing various concentrations of NaCl, resulting in osmotic down-shocks equivalent to withdrawals of between 0 and 760 mM. Cellular survival of strain SMB66 subsequent to the osmotic down-shock is expressed in percentages relative to the viable cell number before the osmotic down-shock. The data given are the means of three independent experiments.

Influence of the alternative sigma factor SigB on cell viability after a hypo-osmotic shock.
Among the three MscS-type putative channel-forming proteins (YkuT, YfkC, and YhdY) of B. subtilis (27), the YkuT protein attracted our special interest because its structural gene has been shown to be under the control of the alternative sigma factor SigB (26), which regulates the general stress regulon of B. subtilis (28). To analyze the influence of a sigB mutation on cell survival subsequent to an osmotic down-shock, we monitored the cell survival of the sigB mutant BLOB22 (sigB{Delta}2::cat). In comparison to that of its isogenic wild-type parent strain JH642, survival of the sigB mutant is not impaired by a sudden osmotic downshift equivalent to the withdrawal of 0.760 M NaCl (Fig. 5). Since the transcription of the ykuT gene is under the control of SigB (26), we introduced the sigB mutation present in BLOB22 (sigB{Delta}2::cat) into the mscL mutant strain SMB53, yielding strain THB7. We compared the survival of THB7 (mscL sigB) with that of SMB66 (mscL ykuT) and found that both strains were highly sensitive to an osmotic downshift (Fig. 5). Hence, the combination of an ykuT mutation or a sigB mutation with an mscL mutation causes hypersensitivity to an osmotic down-shock. These observations indicate that the sensitivity of a sigB mscL double mutant (THB7) is caused by blocking the transcription of the SigB-controlled ykuT gene.


Figure 5
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  		FIG. 5. Survival of B. subtilis wild-type (WT) and ykuT and sigB mutant cells challenged by an osmotic downshift. The cells were pregrown in SMM with 0.8 M NaCl until they reached an OD578 of 0.6 to 0.8. The cultures were then subjected to an osmotic down-shock by a 20-fold dilution in SMM without salt (resulting in an osmotic down-shock equivalent to the withdrawal of 0.76 M NaCl). Cellular survival of the osmotic downshifted strains is expressed in percentages relative to the viable cell number before the osmotic down-shock. The data given are the means of three independent experiments.

Conclusions.
Since their discovery in bacteria, it has been proposed that mechanosensitive channels function as safety valves to prevent cell lysis when a bacterium is rapidly shifted from high-osmolarity to low-osmolarity environments (4, 6). The data presented in this study on the physiological response of B. subtilis toward osmotic downshifts support this notion. We demonstrate in this contribution that the MscL channel functions as the main mechanosensitive solute release system of B. subtilis. The SigB-controlled MscS-type channel formed by YkuT makes a modest contribution to solute release in B. subtilis, but its combination with an mscL mutation enhances the sensitivity of B. subtilis to a drop in the external osmolarity. The channel formed by YkuT might make important contributions to solute release from the cells under special growth circumstances because its structural gene is controlled by the central regulator (SigB) of the general stress regulon of B. subtilis that responds to various environmental cues. The sensitivity of mscL and mscL ykuT mutants to osmotic downshifts unambiguously shows that mechanosensitive channels play a pivotal role in the management of B. subtilis cells in the transition from a hypo- to a hypertonic environment.


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ACKNOWLEDGMENTS
 
We thank V. Koogle for her help in editing the manuscript. We greatly appreciate the expert technical assistance of Jutta Gade, Alexandra Oulds, and Jochen Sohn throughout this study.

The Deutsche Forschungsgemeinschaft (through SFB-395), the Graduiertenkolleg Proteinfunktion auf atomarer Ebene, and the Fonds der Chemischen Industrie provided greatly appreciated financial support for this study. C.B. gratefully acknowledges the receipt of a Ph.D. fellowship from the Région de Bretagne of Rennes (France).


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FOOTNOTES
 
* Corresponding author. Mailing address: Philipps University Marburg, Department of Biology, Laboratory for Microbiology, Karl-von-Frisch-Str., D-35032 Marburg, Federal Republic of Germany. Phone: (49)-6421-2821529. Fax: (49)-6421-2828979. E-mail: bremer{at}staff.uni-marburg.de Back

{triangledown} Published ahead of print on 29 February 2008. Back


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REFERENCES
 
    1
  1. Alcayaga, C., R. Venegas, A. Carrasco, and D. Wolff. 1992. Ion channels from the Bacillus subtilis plasma membrane incorporated into planar lipid bilayers. FEBS Lett. 311:246-250.[CrossRef][Medline]
    2. 2
  2. Bass, R. B., P. Strop, M. Barclay, and D. C. Rees. 2002. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298:1582-1587.[Abstract/Free Full Text]
    3. 3
  3. Berrier, C., A. Coulombe, I. Szabo, M. Zoratti, and A. Ghazi. 1992. Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria. Eur. J. Biochem. 206:559-565.[Medline]
    4. 4
  4. Blount, P., and P. C. Moe. 1999. Bacterial mechanosensitive channels: integrating physiology, structure and function. Trends Microbiol. 7:420-424.[CrossRef][Medline]
    5. 5
  5. Booth, I. R., M. D. Edwards, S. Black, U. Schumann, and S. Miller. 2007. Mechanosensitive channels in bacteria: signs of closure? Nat. Rev. Microbiol. 5:431-440.[CrossRef][Medline]
    6. 6
  6. Booth, I. R., and P. Louis. 1999. Managing hypoosmotic stress: aquaporins and mechanosensitive channels in Escherichia coli. Curr. Opin. Microbiol. 2:166-169.[CrossRef][Medline]
    7. 7
  7. Bremer, E. 2002. Adaptation to changing osmolarity, p. 385-391. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and its closest relatives. ASM Press, Washington, DC.
    8. 8
  8. Bremer, E., and R. Krämer. 2000. Coping with osmotic challenges: osmoregulation through accumulation and release of compatible solutes in bacteria, p. 79-97. In G. Storz and R. Hengge-Aronis (ed.), Bacterial stress responses. ASM Press, Washington, DC.
    9. 9
  9. Britten, R. J., and F. T. McClure. 1962. The amino acid pool in Escherichia coli. Bacteriol. Rev. 26:292-335.[Free Full Text]
    10. 10
  10. Chang, G., R. H. Spencer, A. T. Lee, M. T. Barclay, and D. C. Rees. 1998. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282:2220-2226.[Abstract/Free Full Text]
    11. 11
  11. Cutting, S. M., and P. B. Vander Horn. 1990. Genetic analysis, p. 27-74. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, Inc., Chichester, United Kingdom.
    12. 12
  12. da Costa, M. S., H. Santos, and E. A. Galinski. 1998. An overview of the role and diversity of compatible solutes in Bacteria and Archaea. Adv. Biochem. Eng. Biotechnol. 61:117-153.[Medline]
    13. 13
  13. Dersch, P., H. Fsihi, and E. Bremer. 1994. Low-copy-number T7 vectors for selective gene expression and efficient protein overproduction in Escherichia coli. FEMS Microbiol. Lett. 123:19-26.[CrossRef][Medline]
    14. 14
  14. Folgering, J. H., P. C. Moe, G. K. Schuurman-Wolters, P. Blount, and B. Poolman. 2005. Lactococcus lactis uses MscL as its principal mechanosensitive channel. J. Biol. Chem. 280:8784-8792.[Abstract/Free Full Text]
    15. 15
  15. Guérout-Fleury, A.-M., N. Frandsen, and P. Stragier. 1996. Plasmids for ectopic integration in Bacillus subtilis. Gene 180:57-61.[CrossRef][Medline]
    16. 16
  16. Harwood, C. R., and A. R. Archibald. 1990. Growth, maintenance and general techniques, p. 1-26. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, Inc., Chichester, United Kingdom.
    17. 17
  17. Kappes, R. M., B. Kempf, and E. Bremer. 1996. Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD. J. Bacteriol. 178:5071-5079.[Abstract/Free Full Text]
    18. 18
  18. Kappes, R. M., B. Kempf, S. Kneip, J. Boch, J. Gade, J. Meier-Wagner, and E. Bremer. 1999. Two evolutionarily closely related ABC-transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis. Mol. Microbiol. 32:203-216.[CrossRef][Medline]
    19. 19
  19. Kempf, B., and E. Bremer. 1995. OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. J. Biol. Chem. 270:16701-16713.[Abstract/Free Full Text]
    20. 20
  20. Kempf, B., and E. Bremer. 1998. Uptake and synthesis of compatible solutes as microbial stress responses to high osmolality environments. Arch. Microbiol. 170:319-330.[CrossRef][Medline]
    21. 21
  21. Levina, N., S. Totemeyer, N. R. Stokes, P. Louis, M. A. Jones, and I. R. Booth. 1999. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 18:1730-1737.[CrossRef][Medline]
    22. 22
  22. Martinac, B. 2001. Mechanosensitive channels in prokaryotes. Cell. Physiol. Biochem. 11:61-76.[CrossRef][Medline]
    23. 23
  23. Miller, S., M. D. Edwards, C. Ozdemir, and I. R. Booth. 2003. The closed structure of the MscS mechanosensitive channel. Cross-linking of single cysteine mutants. J. Biol. Chem. 278:32246-32250.[Abstract/Free Full Text]
    24. 24
  24. Moe, P. C., P. Blount, and C. Kung. 1998. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol. Microbiol. 28:583-592.[CrossRef][Medline]
    25. 25
  25. Perozo, E. 2006. Gating prokaryotic mechanosensitive channels. Nat. Rev. Mol. Cell Biol. 7:109-119.[CrossRef][Medline]
    26. 26
  26. Petersohn, A., M. Brigulla, S. Haas, J. D. Hoheisel, U. Volker, and M. Hecker. 2001. Global analysis of the general stress response of Bacillus subtilis. J. Bacteriol. 183:5617-5631.[Abstract/Free Full Text]
    27. 27
  27. Pivetti, C. D., M. R. Yen, S. Miller, W. Busch, Y. H. Tseng, I. R. Booth, and M. H. Saier, Jr. 2003. Two families of mechanosensitive channel proteins. Microbiol. Mol. Biol. Rev. 67:66-85.[Abstract/Free Full Text]
    28. 28
  28. Price, C. W. 2000. Protective function and regulation of the general stress response in Bacillus subtilis and related gram-positive bacteria, p. 179-197. In G. Storz and R. Hengge-Aronis (ed.), Bacterial stress responses. ASM Press, Washington, DC.
    29. 29
  29. Ruffert, S., C. Lambert, H. Peter, V. F. Wendisch, and R. Krämer. 1997. Efflux of compatible solutes in Corynebacterium glutamicum mediated by osmoregulated channel activity. Eur. J. Biochem. 247:572-580.[Medline]
    30. 30
  30. Schleyer, M., R. Schmid, and E. P. Bakker. 1993. Transient, specific and extremely rapid release of osmolytes from growing cells of Escherichia coli K-12 exposed to hypoosmotic shock. Arch. Microbiol. 160:424-431.[CrossRef][Medline]
    31. 31
  31. Sukharev, S. I., P. Blount, B. Martinac, F. R. Blattner, and C. Kung. 1994. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature 368:265-268.[CrossRef][Medline]
    32. 32
  32. Szabo, I., V. Petronilli, and M. Zoratti. 1992. A patch-clamp study of Bacillus subtilis. Biochim. Biophys. Acta 1112:29-38.[Medline]
    33. 33
  33. von Blohn, C., B. Kempf, R. M. Kappes, and E. Bremer. 1997. Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B. Mol. Microbiol. 25:175-187.[CrossRef][Medline]
    34. 34
  34. Wahome, P. G., and P. Setlow. 2006. The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis. Arch. Microbiol. 186:377-383.[CrossRef][Medline]
    35. 35
  35. Wipat, A., and C. Harwood. 1999. The Bacillus subtilis genome sequence: the molecular blueprint of a soil bacterium. FEMS Microbiol. Ecol. 28:1-9.[CrossRef]
    36. 36
  36. Wood, J. M. 1999. Osmosensing by bacteria: signals and membrane-based sensors. Microbiol. Mol. Biol. Rev. 63:230-262.[Abstract/Free Full Text]
    37. 37
  37. Zoratti, M., V. Petronilli, and I. Szabo. 1990. Stretch-activated composite ion channels in Bacillus subtilis. Biochem. Biophys. Res. Commun. 168:443-450.[CrossRef][Medline]

Applied and Environmental Microbiology, April 2008, p. 2454-2460, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.01573-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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