Metagenome-Derived Clones Encoding Two Novel Lactonase Family Proteins Involved in Biofilm Inhibition in Pseudomonas aeruginosa
Appl. Environ. Microbiol. Schipper et al.
75: 224
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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HPLC-MS-DAD analyses of partially hydrolyzed synthetic 3-oxo-C8-HSL and after complete chemical hydrolysis of synthetic 3-oxo-C8-HSL (Fig. S1); HPLC-tandem mass spectrometry profile of the uncleaved substrate 3-oxo-C8-HSL and the N-(3-oxooctanoyl)-L-homoserine with the ring-opened lactone, using recombinant and purified BpiB04 (Fig. S2); swarming complementation of bpi-PAO1 clones with 3-oxo-C12-HSL after 2 days of incubation at 37°C (Fig. S3).
Zipped MS PPT file, 923K.
