
Resolving Genetic Functions within Microbial Populations: In Situ Analyses Using rRNA and mRNA Stable Isotope Probing Coupled with Single-Cell Raman-Fluorescence In Situ Hybridization
Appl. Environ. Microbiol. Huang et al.
75: 234
Supplemental material
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Supplemental methods; DNA/RNA extraction from groundwater (Fig. S1); effect of naphthalene concentrations on groundwater microbial communities (Fig. S2); microbial community profile relating to degradation species (Fig. S3); DGGE profile of microbial communities present in fractions 6 from equilibrium density gradients at 0 to 72 h after the 13C-naphthalene pulse (Fig. S4); 16S rRNA-based sequence diversity of naphthalene degraders isolated from the soil and groundwater of the contaminated site and phylogenetic analysis of NDO gene sequences recovered from groundwater communities relating to the Acidovorax sp. and three Pseudomonas isolates (Fig. S5); shifting of some bands of Raman spectra of individual bacteria as they integrate 13C-naphthalene into the cell (Fig. S6).
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