Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces virginiae IBL-14
Appl. Environ. Microbiol. Wang et al.
75: 4202
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Identification of P450s by degenerate primers (Fig. S1), map of PKC1139 (Fig. S2), inactivation of fcpC (Fig. S3), the 13C-NMR (Fig. S4), 1H-NMR (Fig. S5), HMQC (Fig. S6), and NOESY (Fig. S7) spectra of isonuatigenone, SDS-PAGE analysis of FcpC (Fig. S8), clone of fcpD (Fig. S9), clone of ferredoxin reductase from S. coelicolor A3(2) (Fig. S10), and CO-reduced difference spectrum (Fig. S11).
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