Simultaneous Analysis of Bacterioferritin Gene Expression and Intracellular Iron Status in Pseudomonas putida KT2440 by Using a Rapid Dual Luciferase Reporter Assay
Appl. Environ. Microbiol. Chen et al.
75: 866
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
PCR primers used in this study (Table S1).
MS Word document, 57K.
- Supplemental file 2
-
Bacterial strains and plasmids used in this study (Table S2).
MS Word document, 67K.
- Supplemental file 3
-
Construction of fepA-fes-fluc reporter vector (Fig. S1).
Zipped MS PPT file, 24K.
- Supplemental file 4
-
Construction of Renilla luciferase reporter vectors for tagging of Bfr-encoding genes (Fig. S2).
Zipped MS PPT file, 22K.
- Supplemental file 5
-
Structure of constructs in P. putida KT2440 (Fig. S3).
Zipped MS PPT file, 42K.
- Supplemental file 6
-
Sensitivity and linearity of the dual-luciferase activity in P. putida KT2440 (Fig. S4).
Zipped MS PPT file, 39K.
- Supplemental file 7
-
Stability for firefly and Renilla luciferase activities in vitro (Fig. S5).
Zipped MS PPT file, 20K.
