
Telomere Organization in the Ligninolytic Basidiomycete Pleurotus ostreatus
Appl. Environ. Microbiol. Pérez et al.
75: 1427
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
Schematic representation of telomeric SSP-PCR (Fig. S1).
Zipped MS Word document, 23K.
- Supplemental file 2
-
Estimation of the telomere length in P. ostreatus (Fig. S2).
MS Word document, 22MB.
- Supplemental file 3
-
Flow chart of the experimental approach used to isolate telomeric and subtelomeric sequences using the SSP-PCR technique (Fig. S3).
Zipped MS Word document,11K.
- Supplemental file 4
-
Flow chart of the bioinformatic approach used to identify telomeric and subtelomeric sequences in the 4X assembly of the P. ostreatus genome (Fig. S4).
Zipped MS Word document, 10K.
- Supplemental file 5
-
Amplification products obtained by using PCR primers specific for clon_17 (Fig. S5).
Zipped MS Word document, 1.2MB.
- Supplemental file 6
-
RFLP segregation pattern of the subtelomeric sequence present in clone_22 (Fig. S6).
Zipped MS Word document, 7MB.
- Supplemental file 7
-
RFLP segregation analysis of the amplified fragment of clone_17 (Fig. S7).
Zipped MS Word document, 3MB.
- Supplemental file 8
-
Multiple alignments (Clustal X) of the core domains of selected helicases belonging to the RecQ family (Fig. S8).
Zipped MS Word document, 8K.
- Supplemental file 9
-
Conserved domain architecture of the het gene (Fig. S10).
Zipped MS Word document, 22K.
- Supplemental file 10
-
PCR primers used to amplify telomere-adjacent sequences of different clones (Table S1).
Zipped MS Word document, 7K.
- Supplemental file 11
-
TRFs assigned and not incorporated to linkage groups of P. ostreatus (Table S2).
Zipped MS Word document, 10K.
- Supplemental file 12
-
Gene prediction in telomere-positive phages (Table S3).
Zipped MS Word document, 7K.