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Telomere Organization in the Ligninolytic Basidiomycete Pleurotus ostreatus
Appl. Environ. Microbiol. Pérez et al. 75: 1427

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Schematic representation of telomeric SSP-PCR (Fig. S1).
    Zipped MS Word document, 23K.
  • Supplemental file 2 - Estimation of the telomere length in P. ostreatus (Fig. S2).
    MS Word document, 22MB.
  • Supplemental file 3 - Flow chart of the experimental approach used to isolate telomeric and subtelomeric sequences using the SSP-PCR technique (Fig. S3).
    Zipped MS Word document,11K.
  • Supplemental file 4 - Flow chart of the bioinformatic approach used to identify telomeric and subtelomeric sequences in the 4X assembly of the P. ostreatus genome (Fig. S4).
    Zipped MS Word document, 10K.
  • Supplemental file 5 - Amplification products obtained by using PCR primers specific for clon_17 (Fig. S5).
    Zipped MS Word document, 1.2MB.
  • Supplemental file 6 - RFLP segregation pattern of the subtelomeric sequence present in clone_22 (Fig. S6).
    Zipped MS Word document, 7MB.
  • Supplemental file 7 - RFLP segregation analysis of the amplified fragment of clone_17 (Fig. S7).
    Zipped MS Word document, 3MB.
  • Supplemental file 8 - Multiple alignments (Clustal X) of the core domains of selected helicases belonging to the RecQ family (Fig. S8).
    Zipped MS Word document, 8K.
  • Supplemental file 9 - Conserved domain architecture of the het gene (Fig. S10).
    Zipped MS Word document, 22K.
  • Supplemental file 10 - PCR primers used to amplify telomere-adjacent sequences of different clones (Table S1).
    Zipped MS Word document, 7K.
  • Supplemental file 11 - TRFs assigned and not incorporated to linkage groups of P. ostreatus (Table S2).
    Zipped MS Word document, 10K.
  • Supplemental file 12 - Gene prediction in telomere-positive phages (Table S3).
    Zipped MS Word document, 7K.




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