Files in this Data Supplement:

• Supplemental file 1 - Schematic diagram of the wastewater treatment plant from which the samples in this study were taken (Fig. S1).
  Zipped PDF, 319K.
• Supplemental file 2 - Summary of the bioinformatics analyses used in the study (Fig. S2).
  Zipped PDF file, 173K.
• Supplemental file 3 - Distribution of sequence lengths of the 454-FLX pyrosequence reads from this study (Fig. S3).
  Zipped PDF, 168K.
• Supplemental file 4 - Large contigs produced by the 454 assembler (File S1).
  Zipped FNA file, 32K.
• Supplemental file 5 - Functional assignments for assembled contigs (File S2).
  Zipped TXT file, 7K.
• Supplemental file 6 - Putative 16S rRNA gene sequences within the sequences in our data set as identified by BLAST analysis (File S3).
  Zipped FNA file, 61K.
• Supplemental file 7 - RDP assignments of putative 16S rRNA genes (File S4).
  Zipped TXT file, 19K.
• Supplemental file 8 - The locations of 16S rRNA sequences of samples as determined by manual inspection of GenBank records (File S5).
  Zipped TXT file, 73K.
• Supplemental file 9 - The fraction of each assembled genome at NCBI covered by reads recruited from our sequences (File S6).
  Zipped TXT file, 80K.
• Supplemental file 10 - Figure and file legends.
  MS Word document, 25K.
• Supplemental file 11 - Supplemental bioinformatics methods.
  Zipped MS Word document, 74K.