
Transcription Analysis of Genes Encoding Homologues of Reductive Dehalogenases in "Dehalococcoides" sp. Strain CBDB1 by Using Terminal Restriction Fragment Length Polymorphism and Quantitative PCR
Appl. Environ. Microbiol. Wagner et al.
75: 1876
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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PCR primers used in this study (Table S1); T-RFLP profiles of cluster 2a_1 rdhA genes or transcripts obtained by amplification with the degenerate primer pair 2a_f/2a-1_r and digestion with AluI (Fig. S1); transcription profiles of the housekeeping genes tuf, rpoA, and rpoB and of the 16S rRNA gene during growth with 1,2,3,-TCB (Fig. S2); transcription analysis of five selected rdhA genes, cbrA, cbdbA1624, cbdbA1453, cbdbA1618, and cbdbA187, of strain CBDB1 (Fig. S3); transcription levels of 10 rdhA genes of Dehalococcoides sp. strain CBDB1 48 h after the addition of 1,2,3- or 1,2,4-TrCB (Fig. S4).
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