Ser67Asp and His68Asp Substitutions in Candida parapsilosis Carbonyl Reductase Alter the Coenzyme Specificity and Enantioselectivity of Ketone Reduction
Appl. Environ. Microbiol. Zhang et al.
75: 2176
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Designations, positions, and sequences of oligonucleotides used for mutagenesis reactions (Table S1).
Zipped MS Word document, 8K.
- Supplemental file 2
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SDS-PAGE of cell-free extracts of transformants (Fig. S1).
Zipped TIF file, 2MB.
- Supplemental file 3
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Analytical ultracentrifugation analysis of SCR and S67D/H68D variants (Fig. S2).
Zipped TIF file, 189K.
- Supplemental file 4
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Elution profiles of SCR (Fig. S3A) and S67D/H68D (Fig. S3B) variants in the presence or absence of 0.5 mM NADPH on a Superdex (10/300 GL) column.
Zipped TIF file, 49K.
