Files in this Data Supplement:

• Supplemental file 1 - Description of bacteria, primer and probe design, nucleic acid preparation, quantitative real-time PCR, preparation of standard curves, PCR tests, soil experiments, and data analysis.
  PDF file, 88K.
• Supplemental file 2 - Sequence alignment of the three known classes of tfdA genes represented by sequences with GenBank accession no. M16730, U25717, and AF377325 for classes I to III, respectively, and oligonucleotide probes used in the TaqMan qPCR assay (Fig. S1).
  PDF file, 69K.
• Supplemental file 3 - Standard curves for quantification of 10-fold dilution series of the three classes of tfdA genes by using the three different qPCR approaches (Fig. S2).
  PDF file, 58K.
• Supplemental file 4 - Melting curves of the amplification products obtained from the three classes of tfdA genes by using the two approaches with SYBR green as dye (Fig. S3).
  PDF file, 56K.
• Supplemental file 5 - Selected properties of soils (Table S1).
  PDF file, 65K.
• Supplemental file 6 - qPCR standard curve parameters (Table S2).
  PDF file, 9K.
• Supplemental file 7 - Quantities of tfdA genes measured in the 13 soils after mineralization of different PA, using the TaqMan probe assay (Table S3).
  PDF file, 25K.