Application of Recognition of Individual Genes-Fluorescence In Situ Hybridization (RING-FISH) To Detect Nitrite Reductase Genes (nirK) of Denitrifiers in Pure Cultures and Environmental Samples
Appl. Environ. Microbiol. Pratscher et al.
75: 802
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Legends to Fig. S1 to S6.
MS Word document, 22K.
- Supplemental file 2
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Hybridization efficiency of homologous and heterologous nirK polynucleotide probes to Alcaligenes xylosoxidans cells and secondary structures of A. xylosoxidans probe (Fig. S1).
Zipped PPT file, 43K.
- Supplemental file 3
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Hybridization efficiency of homologous and heterologous nirK polynucleotide probes to Hyphomicrobium aestuarii cells and secondary structures of H. aestuarii probe (Fig. S2).
Zipped PPT file, 43K.
- Supplemental file 4
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Hybridization efficiency of homologous and heterologous nirK polynucleotide probes to Pseudomonas sp. strain G-179 cells and secondary structures of Pseudomonas sp. probe (Fig. S3).
Zipped PPT file, 44K.
- Supplemental file 5
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Hybridization efficiency of homologous and heterologous nirK polynucleotide probes to Sinorhizobium meliloti cells and secondary structures of S. meliloti probe (Fig. S4).
Zipped PPT file, 45K.
- Supplemental file 6
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RING-FISH with nirK-specific polynucleotide probe and nirK-negative strains (Fig. S5).
Zipped MS Word document, 2MB.
- Supplemental file 7
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Gene tree of nirK sequences from pure cultures and from sewage sludge (Fig. S6).
Zipped PDF file, 363K.
