High Rate of N2 Fixation by East Siberian Cryophilic Soil Bacteria as Determined by Measuring Acetylene Reduction in Nitrogen-Poor Medium Solidified with Gellan Gum
Appl. Environ. Microbiol. Hara et al.
75: 2811
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Identified soil bacteria obtained as culturable on MWA’ containing 0.25% D-glucose and 0.25 DL-malic acid (Table S1); use of gellan gum as a matrix to increase acetylene reduction of microfloral communities in Shizunai farmland Andisol (Table S2); appropriate temperature for N2 fixation by cryophilic bacterial microbiota (Fig. S1); phylogenetic alignment for 16S rRNA gene sequences of soil bacteria from East Siberian forest bed soil (Fig. S2); validation of correlations between acetylene reduction and bacterial cell growth (Fig. S3).
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