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Appl Environ Microbiol. 1967 July; 15(4): 839-843
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Preparation of ¹⁴C-labeled Tuberculin Purified Protein Derivative

Connaught Medical Research Laboratories, University of Toronto, Toronto, Ontario, Canada

ABSTRACT

¹⁴C-labeled tuberculin purified protein derivative (¹⁴C-tuberculin PPD) has been prepared from culture filtrates of Mycobacterium tuberculosis var. hominis grown in a culture medium containing uniformly labeled ¹⁴C-amino acids. With a mixture of ¹⁴C-amino acids (an acid hydrolysate of ¹⁴C-Chlorella protein) in the medium, the recoveries of ¹⁴C in the final product were higher than with ¹⁴C-labeled-L-glutamic acid. ¹⁴C-tuberculin PPD was separated into tuberculoprotein and nucleic acid by paper electrophoresis. The specific radioactivity of tuberculoprotein was substantially greater than that of the nucleic acid. ¹⁴C-tuberculin PPD is advocated as a means to measure the adsorption of tuberculin to glass or other surfaces. It could also prove useful as a means to study the structure and mode of action of tuberculin.

¹ Present address: Microbiology Division, Food and Drug Directorate, Department of National Health and Welfare, Ottawa, Canada.