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Appl Environ Microbiol. 1973 September; 26(3): 309-313
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Staphylococcal Enterotoxins A and B: Solid-Phase Radioimmunoassay in Food

Food and Drug Administration, Division of Microbiology, Cincinnati, Ohio 45226

ABSTRACT

An immunoassay employing ¹²⁵I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per g of food; nonspecific inhibitions were 15% or less.