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Appl Environ Microbiol. 1974 February; 27(2): 368-374
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Slide Agglutination Method for the Serological Identification of Neisseria gonorrhoeae with Anti-Gonococcal Antibodies Adsorbed to Protein A-Containing Staphylococci

Dan Danielsson and Göran Kronvall

Department of Clinical Bacteriology, Central County Hospital, Örebro, Sweden
Department of Medical Microbiology, University of Lund, Lund, Sweden

ABSTRACT

A rapid slide agglutination test has been developed for the identification of Neisseria gonorrhoeae that are primarily detected as oxidase-positive colonies in gonococcal cultures. The technique is based on the specific nonimmune reactivity between the Fc portion of immunoglobulin (Ig)G and staphylococcal protein A. IgG molecules adsorbed to stabilized staphylococci will thereby become oriented with their antigen-reactive sites that are directed outwards. Protein A-containing staphylococci with unabsorbed anti-gonococcal antibodies gave positive co-agglutination reactions with gonococci but also with meningococci, some Moraxella, Haemophilus, and Pseudomonas strains. These crossreactions were eliminated by absorption of the anti-gonococcal antiserum with meningococcal and Moraxella organisms prior to the coating of reagent staphylococci. In the routine culture diagnosis of N. gonorrhoeae the use of specific gonococcal reagent staphylococci gave concordant results with fermentation procedures and immunofluorescent techniques.

Appl Environ Microbiol. 1974 February; 27(2): 368-374
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.