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Appl Environ Microbiol. 1974 February; 27(2): 425-426
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Heat Injury and Recovery of Vegetative Cells of Clostridium botulinum Type E

M. D. Pierson, S. L. Payne and G. L. Ades

1 Department of Food Science and Technology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

ABSTRACT

Vegetative cells of Clostridium botulinum type E (Tenno) were heated at 40.5 C in a prereduced peptone-yeast extract broth (PY). During the heating period, cell numbers remained essentially constant for 3 h as indicated by roll tube counts in PY agar (PYA); however, injury and recovery from injury were observed when the cells were enumerated using PYA containing either 0.06 or 0.07% bile salts.

Appl Environ Microbiol. 1974 February; 27(2): 425-426
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.