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Appl Environ Microbiol. 1974 August; 28(2): 298-302
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Rapid Diagnosis of Infection by Gas-Liquid Chromatography: Analysis of Sugars in Normal and Infected Cerebrospinal Fluid

Stanley Amundson, Abraham I. Braude and Charles E. Davis

Department of Medicine and Pathology, University of California, San Diego, California, 92103

ABSTRACT

A highly reproducible procedure was developed for gas-liquid chromatographic analysis of trimethylsilyl derivatives of normal human cerebrospinal fluid. Fourteen normal human cerebrospinal fluid samples tested with this procedure contained {alpha}- and ß-glucose as well as isomers of two unidentified sugars. Chromatographic changes in three cases of meningeal inflammation (two cryptococcosis and one thalamic astrocytoma) were limited to decreased concentrations of all sugars. In one case of early meningitis, the concentrations of the unknown sugars decreased before glucose. Now that a reproducible chromatogram of the trimethylsilyl derivatives of normal human cerebrospinal fluid has been established, more samples of abnormal cerebrospinal fluid should be prepared by these methods and examined by gas-liquid chromatography. It may be possible to identify unique products of infectious agents which will permit rapid diagnosis of central nervous system infection.

Appl Environ Microbiol. 1974 August; 28(2): 298-302
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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