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Appl Environ Microbiol. 1979 July; 38(1): 66-71

Continuous Cultivation for Apparent Optimization of Defined Media for Cellulomonas sp. and Bacillus cereus

R. J. Summers, D. P. Boudreaux and V. R. Srinivasan

Department of Microbiology, Louisiana State University, Baton Rouge, Louisiana 70803

ABSTRACT

Steady-state continuous culture was used to optimize lean chemically defined media for a Cellulomonas sp. and Bacillus cereus strain T. Both organisms were extremely sensitive to variations in trace-metal concentrations. However, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumentation. The optimized media supported critical dilution rates of 0.571 and 0.467 h^–1 for Cellulomonas and Bacillus, respectively. These values approximated maximum growth rate values observed in batch culture.

Present address: Molecular Biology Section, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20205.