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Appl Environ Microbiol. 1979 October; 38(4): 758-760

Calculation of Km and Vmax from Substrate Concentration Versus Time Plot

G. H. M. Counotte and R. A. Prins

1 Laboratory of Animal Nutrition, State University of Utrecht, Yalelaan 1, Utrecht, The Netherlands

ABSTRACT

A simple method for the calculation of kinetic parameters (Km, Vmax) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.

Appl Environ Microbiol. 1979 October; 38(4): 758-760






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