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Appl Environ Microbiol. 1983 April; 45(4): 1389-1393
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Sporulation of Clostridium acetobutylicum P262 in a Defined Medium

Susan Long, David T. Jones and David R. Woods

Department of Microbiology, University of Cape Town, Rondebosch, 7700, South Africa

ABSTRACT

A defined minimal sporulation medium for Clostridium acetobutylicum P262, which produces high levels of solvents, is described. The overall sporulation sequence was similar to that of other endospore-forming bacteria. However, we observed a presporulation stage, during which swollen phase-bright cells which contained large amounts of granulose formed. During sporulation, the initiation of spore coat formation occurred before the onset of cortex formation. Other Clostridium strains tested showed marked variations in ability to grow and sporulate in various minimal media.

Appl Environ Microbiol. 1983 April; 45(4): 1389-1393
Copyright © 1983, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tracy, B. P., Gaida, S. M., Papoutsakis, E. T. (2008). Development and Application of Flow-Cytometric Techniques for Analyzing and Sorting Endospore-Forming Clostridia. Appl. Environ. Microbiol. 74: 7497-7506 [Abstract] [Full Text]  
  • Alsaker, K. V., Papoutsakis, E. T. (2005). Transcriptional Program of Early Sporulation and Stationary-Phase Events in Clostridium acetobutylicum. J. Bacteriol. 187: 7103-7118 [Abstract] [Full Text]  


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